Science topic
Biotechnology - Science topic
Biotechnology
Questions related to Biotechnology
Dear colleagues,
On behalf of the organizing committee I with great pleasure inviting you to join our virtual event:
On the 2nd and the 3rd of May this year we are organizing the 3rd CZU hybrid seminar "Biotechnology in small ruminant reproduction: an international experience". With this, we are going to cover aspects of small ruminants' artificial insemination, ram and buck sperm cryobiology, ram and buck sperm liquid storage, analysis of sperm quality by modern analytical methods, sheep and goat breed conservation, and some others.
The aim of the seminar, firstly, is to give the younger generation of scientists and students an overview of the modern methods currently used in top-level laboratories around the world, and secondly to build even tight bonds between several laboratories working in a similar field.
To join the seminar, you do not need a registration (no registration fee applied). The MS Teams will be used as a platform for the online seminar. Please, find the link and the scientific program together with some important info on this webpage: https://katedry.czu.cz/en/ksz/animal-reproduction-sperm-cryopreservation-and-analysis-2024
Thank you very much in advance! We are waiting for your attendance!
Greetings from Prague, Czechia!
Sincerely,
Filipp Georgijevič Savvulidi
Martin Ptáček
Seminar Organizing Committee
(in case of any questions on the seminar, please, do not hesitate to contact us by [email protected])
i need any information or stories about Lawsuits basis of biotechnology in turkey
How are these exploited in biotechnological applications?
I have review article ready for submission titled as "A Comprehensive Review on Crop Modification Techniques using Traditional and Modern Biotechnological Approaches". i want some collaboration to submit it in well reputed journal. email :[email protected]
Deep Biotech is considered a part of deep tech because it involves complex scientific research, innovation, and advanced engineering, often leading to breakthroughs that can revolutionize multiple industries and address some of society's most pressing challenges.
source: google: connection between biotechnology and deep tech
The next big surge of innovation powered by emerging technologies and the approach of deep tech entrepreneurs. Its economic, business, and social impact will be felt everywhere because deep tech ventures aim to solve many of our most complex problems.
The great wave encompasses artificial intelligence (AI), synthetic biology, nanotechnologies, and quantum computing, among other advanced technologies. But even more significant are the convergences of technologies and of approaches that will accelerate and redefine innovation for decades to come.
As technological advances move from the lab to the marketplace, and as companies form to pursue commercial applications, we see a number of similarities in how and why they are being developed—and a powerful ecosystem is taking shape to drive their development. We witnessed the power of that ecosystem in the year just ended, as Moderna and the team of BioNTech and Pfizer separately took two COVID-19 vaccines from genomic sequence to market in less than a year. Although these companies did remarkable work at unheard-of speed, they benefited from the work of many others, including governments, academia, venture capital, and big business. All of these are critical players in the coming wave.
Would you contribute to a new journal through papers, editorial board, and reviewing? What is your opinion?
Fungal Biotech https://whatsapp.com/channel/0029VaJT1WA90x34GtHny92D
Welcome to "Fungal Biotechnology" on WhatsApp! 🍄🔬 Explore the fascinating world of fungi and their applications in biotechnology. 🌱💡 Stay updated on cutting-edge research, breakthroughs, and discussions in the field. Let's dive into the mycelial network of knowledge together! 🌐 #FungalBiotech #ScienceChat
Genome editing (also called gene editing) is a group of technologies that give scientists the ability to change an organism's DNA. These technologies allow genetic material to be added, removed, or altered at particular locations in the genome. Several approaches to genome editing have been developed. A recent one is known as CRISPR-Cas9, which is short for clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9. The CRISPR-Cas9 system has generated a lot of excitement in the scientific community because it is faster, cheaper, more accurate, and more efficient than other existing genome editing methods.
Hi everyone.
As crude as this may sound, I really need a job. Its been far too long I've been trying various portals and methods including LinkedIn, Jobstreet, indeed, recruiters, conference networking etc etc etc. I have even been trying for postgrads for a year now, however I have lost interest as earning is my primary concern.
I have a GPA of 3.77 (first class honors), IELTS Band 8, have diverse skillsets, and can adapt to any assigned tasks and new environments.
I'm looking for anything related to healthcare biotechnology research, lab assistance or even scientific communication and exhibitions.
I am open to opportunities in Singapore as I am planning to visit by mid Jan 2024. Other locations of interest include the UK, Australia, New Zealand and Europe.
I would greatly appreciate any opportunities that members of our fellow science community may know.
Please refer to the attached CV for what I have accomplished thus far. Please feel free to contact me at [email protected] for further discussion as i am not too active on Researchgate.
In advance, I'd like to thank you for your willingness and assistance on the matter.
Regards,
Zahraa
Years back, I have taken notes from that book for my ug assignment, but I missed to note down the author’s name.
Now, I need the book for further reference. I searched a lot but i could not find it.
I could not get the book without knowing the author’s name.
BOOK DETAILS
TITLE: TEXTBOOK OF MICROBIOLOGY
CHAPTER 29: TOOLS OF FERMENTATION TECHNOLOGY ON PAGE 1021
If anyone knows the author details and the edition of the book, please share it.
Thank you in advance.
while studying about gene editing techniques, I came to know about the term targeted mutagenesis.
it was explained that after cutting the DNA at specific site using engineered nuclease, if homologous DNA not provided it leads to NHEJ which in turn leads to random mutation at the cut site. this was called as targeted mutagenesis.
my doubt is whether targeted mutagenesis and site specific mutagenesis are same are different.
please quote some references
I am planning to expand my business and hope to learn some industry information from you in your area. Or share some resources and explore potential cooperation opportunities.
#Medicine #Biotechnology #Doctor#Research
Dear friends, I'm looking for colleagues who want to write a book on current topics in biotechnology. I'm attaching the possible abstract of the book, in case any of you want to participate.
1. Proposed title of the book:
Research and drug development in human diseases- Recent advances in biotechnology
2. A one-page abstract outlining its key features and information about the intended audience:
The research in human diseases is a very active field in health sciences. The development of new drugs with improvements in efficacy as well as the reduction in side effects is desirable in drug development. In this regard, the research about of new bioactive compounds, which can be further modified to improve its medical performance is It is a topic of current research.
The fundamental aspect of the research work about new drugs includes the isolation and identification of organic molecules in different sources. This assignment can be drastically improved by the use of modern biotechnology techniques as the omic sciences, for example.
Also, the research in the application of the isolated compounds can be from different sources as natural, synthesis and in silico among others sources. Therefore, there are several biological models to study the new drugs. For example, the mourin model is useful to study the tissue and physiological functions. Others biological models as the chicken egg model is related to the antibody and vaccine research, as well as the fish model which is useful to study cell morphology. Although, the biotechnology is used for the development of new drugs recently.
For this manner, In the case of vector-transmitted diseases, the identification of the vector via biotechnology is crucial to the development of new drugs. Also, the climatic change facilitates the emergence of new diseases for example the chikungunya and Leishmaniasis diseases, inclusive in countries non-tropical.
In order to complete these preliminary studies of new drug development, further computer auxiliary models performed by pharmacokinetic studies contributes to our understanding the principal pathways of new drugs in the human system with biotechnology development in the implementation.
This book represents a small but representative effort to go a step ahead in this battle between disease and targets, with the use of biotechnology in the development and implementation of the new drugs. Additionally, this book is intended to give a varied context, which could be useful for health scientists, as well as science students interested to increase its knowledge in frontier research regarded with drug development, particularly in the biotechnology field.
3. Tentative table of contents.
In construction...
If you are interested in collaborating project on Biotechnology, Bionanotechnology, Microbiology, Plant Tissue Culture etc., Please let us know.
Email: [email protected]
I recently conducted a liquid chromatography-mass spectrometry (LC-MS) analysis of my protein sample, which resulted in the identification of over 300 proteins. I need assistance in identifying any novel proteins within this dataset. Can someone guide me through the necessary steps and offer insights on how to interpret the results?
The most common MSCs are: bone marrow MSCs, adipose MSCs, placental MSCs, amniotic membrane MSCs, umbilical cord MSCs, and so on. Clinical studies have shown that these stem cells have therapeutic potential and promote the regeneration and repair of aging tissues.
Any papers on this would be appreciated.
Graduation projects
animal biotechnology
plant biotechnology
microbial biotechnology
bioinformatics
What is the negative control, the positive control and the reagent control in the agarose gel electrophoresis that is prepared for the identification of PCR products? Could a ladder of DNA be a positive control? Could the components of the PCR kit (buffer+ taq polymerase+ dNTPs+ MgCl2) be the reagent control?
The question is very specific so let me give the context. I prepared magnesium ferrite nanoparticles doped on carbon and conducted delignification tests with them (using coconut coir fibre as biomass and hydrogen peroxide of 6%w/v). There was one control (without nanoparticles and only peroxide) and three flasks with nanoparticles and peroxide. To assess the delignification, i checked the total phenol content after 24hrs of incubation of the flasks on a shaker using FC reagent colorimetric method. I observed that the absorbance of the control was higher than the flasks with the nanoparticles. What could be the reason behind this? How do I find out why the delignification was less?
Especially one that involves a biotechnological route
What are the career option available for Fisheries Bio Technology Professionals in abroad and and in Inda?
Dear All,
Biotechnologists from The area of Biotechnology.
Currently I am serving as HoD in Department of Biotechnology, IMSEC, New Delhi NCR.
Is there any person who can help me for two or three month research exposer to any of the labs in any campuses in the USA?
Please respond. That will be a great help.
Thankyou.
Dr Siddharth Vats
Associate Professor (Biotechnology)
HoD Department of Biotechnology IMSEC, New Delhi NCR.
email id: [email protected]
I am a MS thesis student in Plant Breeding and Biotechnology Labratory, University of Dhaka.
How the Fisheries Bio Technology helps in the production of Shrimp Culture In India?
Biodesalination is a concept that combines bioremediation and desalination processes to address water scarcity issues by removing salts and other contaminants from brackish or seawater. Traditional desalination methods, such as reverse osmosis, are energy-intensive and can have environmental impacts. Biodesalination aims to harness biological processes to make the desalination process more sustainable, eco-friendly, and cost-effective.
In biodesalination, biological organisms, such as certain types of bacteria, algae, or plants, are used to reduce the salinity of water. These organisms can either absorb salts directly from the water or facilitate the precipitation of salts, making it easier to separate them from the water. By leveraging the natural abilities of these organisms, biodesalination has the potential to reduce the energy requirements of desalination processes and minimize the environmental impact associated with conventional methods.
Research in the field of biodesalination is ongoing, focusing on identifying suitable biological agents, optimizing the conditions for their growth and salt removal abilities, and developing practical applications for large-scale desalination plants. The integration of biotechnology and desalination techniques holds promise for providing a sustainable solution to the global water scarcity challenge.
Please visit https://www.iipseries.org for more details regarding this book. Submit the chapter using the link of our book series: https://www.iipseries.org/fullviewdetails.php?id=532&title=iipv3ebs17g77-futuristic-trends-in-biotechnology. Make sure to enter my Editor reference id (IIPER1679934610) while submitting the chapter.
Dear Colleagues,
Here's an opportunity to contribute chapters for the forthcoming book that I'm editing, entitled “Futuristic Trends in Biotechnology” to be published by IIP International Publishers, USA and India. I would like to take this opportunity to cordially invite you / your team to submit your unpublished, original work that aligns with the title of the book for consideration for publication.
We already received some good chapter proposals in diverse areas such as Bioremediation, Biofuel, Clinical Engineering, Fermentation Technology, and Metagenomics and are looking for more. The last date for Full chapter submission is 28th August 2023.
Please encourage your colleagues also to contribute. I am hopeful that contributions from your professional circles on this topic would make excellent additions to this publication. Please also feel free to let me know if you'd like to help as a reviewer or if you have any other ideas for collaboration, now or in the future.
Please visit https://www.iipseries.org for more details regarding this book and to submit your work. If you have any questions or concerns, please do not hesitate to contact me. Thank you very much for your consideration of this invitation, and I hope to read your chapter proposal soon.
With every best wish,
Dr. Ali Asger Bhojiya
Dear All,
Ph.D. full-time position in Bangalore with fellowship:
Eligibility: M.Sc. Chemistry/Biochemistry/Biotechnology/Microbiology/Bioinformatics with first class of 60%.
GATE or UGC-NET or UGC-CSIR or SLET or JRF should be qualified.
RS 25,000 per month for full three years will be given.
For further details, contact me on: +919182864256. Call or what's app me for further details.
Is there any manual way to isolate recombinant fosmid DNA from E.coli cells in the absence of isolation Kit?
If natural selection has an impact on modern man even with medecine or biotechnology...
I Want to know biotechnological as well as biochemical approaches for these studies.
We are focusing on Biotechnology, Food Technology, and Molecular Biology students.
How many grams of K2Cr2O7 to dissolve it in 1 liter Distilled water to obtain 50 ppm of Chromium? to become aqueous solution, Is there a specific equation to apply? Thanks
Ali
I saw these on a same step of conversion of Malonyl CoA to Malonyl-ACP. Could anyone clarify this? I add that reference link below.
KEGG PATHWAY: Fatty acid biosynthesis - Chlorella variabilis (genome.jp)
Hello to all my fellow Biologists.
I have resorted to posting this question as my last desperate attempt to find a place for myself in the world of biotechnology.
I am a first class student with relatively good grades (GPA 3.77/4.00) in BSc. of Biotechnology (Hons), excellent extra-curriculars and competitions under my belt, 6 months of work as a Field/Research Assistant and 4 years of previous work experience in events management. I have experience in the microbiology, molecular biology, antivirals, nutraceuticals and cell culture disciplines. I have also taken a great liking to scientific communication and create visual content to make biology simpler for the Layman, both bother and in my own time.
Despite all this, I haven't had a single postgraduate application succeed for the last 2 years. And though I do understand there is high competition for available spots, I also wonder what I may be lacking despite some telling me I have an "impressive CV" and can do a direct PhD.
It is unfortunate however that my family is not doing financially well, therefore I can only afford opportunities with a scholarship or that are work/salary-based. Perhaps this narrows available opportunities but regardless, studentship scholarships have very evidently not opted for me, simply because "there were too many applicants this time around". Perhaps lacking funds is not enough of a criteria? (Hint of sarcasm).
Additionally, I was born and raised in the UAE (I do not get citizenship), therefore I am also looking for a potential country to eventually settle down in while doing the work I love.
I would greatly appreciate if anyone would know of opportunities I may be able to apply for like fully funded PhDs, or skilled/summer programs and workshops/internships, or even Research or Lab assistant positions you or someone you know may be looking for, because unfortunately, I'm 2 rejections away from being completely out of options.
I would greatly appreciate any input you may have or can share with me! I have also added my CV for your reference.
I don't want my impression of the field I love to be tainted with nothing but rejections, and to settle for a job outside our field simply because I had no other choice.
I look forward to hearing from you all.
Sincerely,
Zahraa Ozeer
Hello Dear professors,
I hope you are doing well. In fact, I need your help in getting a post-doctoral position in Food science and Biotechnology or any related branch of science.
I have a list of SNPs for a rice gene. And I have two questions related to this:
1. I want to make a figure showing the respective position of the haplotype in the genomic region (UTR, exon, intron). How can I do it?
2. If not manually, how can I replace the original sequence nucleotides with the haplotype's SNPs?
Hi, I did 16s rRNA sequencing and my isolate showed b.breve strain 3018. After doing the nacl resistance test it turned out that my strain is resistant at concentrations of 1.5%, 2.5%, up to 3.5%. My friend and I have bifidobacterium breve isolate with different strains but our nacl resistance ability is different, my friend's isolate is not resistant to nacl. my question is there a difference in the gene/genome or what? If yes, what is the name of the gene/genome? because I find it difficult to find specific literacy mentioning the genes/genomes that play a role in nacl resistance
note: I did a nacl test for probiotic characterization
Thank You
How can plant breeding and biotechnology be used to develop crop varieties that are resistant to pests and diseases, drought-tolerant, and have higher yields?
- possible answers should be in line with biotechnology in microbial degradation of contaminants
How does the use of genetic engineering and biotechnology affect crop production and food security?
I am trying to isolate a nuclear protein NF-kB from raw cells in order to do a western blot.
I have searched some protocol for nuclear isolation and finally came up with one. But in that protocol, apart from adding 420mM Nacl for NE buffer , they have added an extra 400mM using 5M NaCl directly onto nuclear pellet and again 1 pellet volume of NE buffer. Can anyone tell why extra NaCl should be added, and if we add double the volume of NE buffer, won't the protein get diluted? I'm attaching that protocol along with this.
I repeatedly get no colonies using the GeneMorph II random mutagenesis kit.The initial error prone PCR yields a substantial amount of target DAN, however, after the EZclone step, I get no colonies. Does anyone know what could be going wrong?
Alan
Hello dear biologists and biotechnologists,
you should understand my question and my thinking.
What do you think of the excessive involvement of other disciplines (especially mathematics) in publications in the field of biology, is this not a danger for our dear discipline: biology and biotechnology? . How could we explain that there are in certain cases, potentially, more publications in fields of biology, made by mathematicians than by biologists? Do mathematicians no longer manage to publish in mathematical fields that they turn to biology? I'm afraid that before long, real publications by biologists will be very rare. Save our discipline against opportunism.
I have few sets of questions related to CHO cells (CHO-S cell line) :-
1) Can somebody tell me what effect physical factors like light (other than UV or radiation) have on CHO cell growth, viability, morphology, and quality profile?
2) Can anybody with a microbiology or algal background provide a set of light intensity or LUX ranges for observing any difference in the profile of CHO cells? Though this physical agent appears to be ineffective, as light has no influence on the growth profile of the CHO cells but for an observation and to monitor the growth profile I wanted to setup this experiment.
3)Can someone provide some strategies for installing a tube lamp or a light source inside a CO2 incubator such that the shaking flask with CHO cells is constantly exposed to light and the effect can be easily monitored?
4) Is there any research paper or review paper on effect of light on cells specifically CHO cells, or any other cells which shows a remarkable impact on the overall profile?
if gene expression is low in specific diseases based on previous research,
But when I measured the protein expressed by the same gene in blood of same disease by ELISA I found high serum level?
what is the explanation for this difference between gene expression and its protein concentration in blood?
Dear colleagues,
I am pleased to inform you that our previous project on biotechnology and drug development is progressing well, and I wanted to thank you all for your contributions to its success. Your expertise and insights have been invaluable, and I am grateful for your ongoing support.
With that in mind, I am now excited to announce that I am planning another project on a similar topic, and I would like to invite you to participate once again. This project will focus on the latest developments in biotechnology and their applications in drug discovery and development.
I believe that our collective expertise and diverse perspectives will be critical to the success of this project, and I am looking forward to working with you all again.
I am excited to announce that I am planning to edit a new article collection about natural products and biotechnology. The goal of this collection is to bring together cutting-edge research on the use of natural products in biotechnology, including applications in drug discovery, agriculture, and environmental science.
I am currently seeking to form an editor team and I would like to invite you to participate. The team will consist of 3-5 co-editors, and we aim to have a geographically diverse range of institutions.
To be considered for this role, all Topic Editors must have a PhD and at least 3 years of post-doctoral experience, as well as some relevant editorial experience. The majority of the team must also be at Associate Professor level or above.
In addition, I am writing to let you know that we are actively seeking authors for this collection, and we would be honored to consider your work for inclusion.
Please note that there is a publication charge associated with this collection, as our journal is an open access publication. However, we also understand that funding can be a major barrier for many researchers, which is why we offer a fee waiver program. This program allows authors to apply for a discount of up to 100% on the publication fee, based on their individual financial circumstances.
We believe that every researcher should have the opportunity to share their work with the scientific community, regardless of their financial situation. Our fee waiver program is just one of the ways that we are working to make this a reality.
If you are interested in submitting a manuscript to this collection, please don't hesitate to reach out to me for more information. We would be delighted to consider your work for publication.
Best regards,
Dr. Israel Valencia Quiroz
Laboratorio de Fitoquímica, UBIPRO,
FES Iztacala, UNAM.
We know that putting repetitive sequences in a single plasmid may initiate recombination in those plasmids. If recombination happens, the sequences in between those repetitive sequences may get excised out.
Can this be prevented by having the repetitive sequences in two separate plasmids (with two different backbones)? Would recombination still occur in between the separate plasmid systems?
P.s. I am working with a bacteria which is almost genotypically identical to its wild type variant. It is also not a recA- strain.
I need to observe the fecal egg reduction in rats that I'm studying. I need a protocol how to do it without a McMaster slide.
Dear colleagues,
I am writing to inquire if anyone knows how and where to find some unused antibody that was discontinued by Santa Cruz Biotechnology (KCNQ1 Antibody (C-20): sc-10646). Our lab would like to use it to complete some previous results that we obtained with that particular antibody. SCBT has an alternative antibody as a replacement, but we would prefer to use the old one if possible.
If anyone has any remaining stock of this antibody, or if you know of a source where it may still be available, please let me know. Your assistance in this matter would be greatly appreciated.
I am carrying out polymorphism studies using ssr markers and running them on 6% polyacrylamide gel using 1x tbe buffer. But I'm facing smearing type of bands and multiple bands in certain samples can anyone kindly answer why this problem is encountering
I'm looking for a bacterial promoter/transcription factor which can sense Acetyl-CoA.
Thank you in advance.
We have a Flask that contains broth, and we want to inoculate it with Bacteria inoculum, Can we simply take a touch by the loop or by micropipette?
How helpful is biotechnology for the beneficiation and purification of iron ores? Especially concerning the main impurity like Phosphorus.
is there a specific ratio to follow during the addition?
Hi, I am looking for a company operating in the field of artificial meat. By operating, I mean production or advanced stage of pre-production.
I am looking for because we have some good solutions to offer to this kind of company.
Thank you.
One of my known claims to have been granted a German Patent in 23 days. Is it really possible to obtain a German Patent in such short interval of time?
How would you consider both of them before proceeding with site-directed mutagenesis to screen for improved mutants?
If someone is granted with utility model can he/she claim it to be granted a Patent?
Is it legally acceptable?
Can anyone tell me the procedure to apply for German Patent?
How long does it take grant the patent?
Hi,
I would be grateful if anyone could help me to know about conferences on biomedical engineering (Biotechnology, Bioinformatics, medical physics ... )
Thank you,
Hi I'm looking for co-authors to write a chapter of a book. In particular, the topic is “3D printing techniques and use in the Biotechnology laboratory“ maximum 20 pages.
I deal with direct communication with the publisher and with the revision of the chapter.
The deadline is for 22th November and the total cost is 350 GBP.
My direct contact is [email protected]
I am writing a review article in biotechnology and as you know graphic images are so important in these papers. I would appreciate it if you suggest the best options. Thank you
I'm passionate in oncology research. I did bachelor in Zoology and now pursuing MPhil Molecular Biology and Biotechnology and working on cancer genetics. I am greatly interested to do PhD in cancer research from a world renowned institute but I think with this profile I would get a position in top ranked institute for PhD. Should I go for another master from a renowned foreign institute with major in oncology?
Thanks
The purpose of using those images for the advertisement of streams e.g Biotechnology, Microbiology, Nanotechnology, Ecology
3 dpf larvae of zebrafish will be used to evaluate the body burden concentration of a compound at different period of time.
Recently I have been working on a research project, and the project comprises formulating an ointment, but I am clueless about how to develop one, it would be a very noble help if someone could guide me through with that.
hello
Please introduce me the companies that provide biotechnology services such as designing different types of primers, NGS, RNASeq, etc.
Sometimes all of scientific articles are not available in google search even in another search engine. But when I intended to do a new work, so how can I know that this is first time. Even though, it may be that the work has been done before but it did not appear in my search engine. So, what could basis that we can say this is first time work or research.
Dear Researcher,
i am looking for Molecular Biotech lab whole equipments and consumables and chemicals list specially with its brand and catalog Number. Can anyone provide me. Suppose if anyone has RKVY project equipments list details also no issues
I am doing a literature survey on some disease related polymorphisms, +1730 G/A and +1082 G/A are tow single nucleotide polymorphisms (SNPs) in estrogen receptor 2 gene. How can I get their rs (reference SNP)? there are no rs mentioned in the published papers.
I couldn't see much paper where plant breeders use biochemical such as proline content Malondialdehyde (MDA) and dyes such as NBT or DAB( for ROS detection) for screening stress-tolerant accession on a large scale (100-200 which I suppose is possible to do). Are not these methods better than phenotyping grain yield, biomass, plant height, NDVI, LAI, etc?
Can anyone suggest a method to quantify proteins in melanin containing samples?
*keeping in mind that lab work isn't possible
Hi,
I wonder if anyone knows how to use sample release reagent from Sansure Biotech ?
Long story short, I need to degrade 30ug of RNA and i need to do it at 4C. I want to use only as much RNAse A as necessary.
So if performing the reaction at 4C, how long of incubation time and how much RNAse A would i need to degrade 30ug of RNA?
For example, would 1ug of RNAse A(20ug/ml conc.) for 15min at 4C be enough?
Hi!
Dear colleagues and respected seniors, I am searching for a biochemical reaction to identify the km and vmax values for the said enzyme, which converts alpha carotene into Lutein, but still I am not able to identify, so if anyone could help to find it, give me a reference article/ paper please, I will be very thankful to al of you.
Thanks and best regards
Mr. Rafi Ullah Khan
PhD candidate (Biotechnology)
Hello...
Two months ago, I bought pyrocystis fusiformis culture for my research. Unfortunately, no bioluminescence was observed, and the culture seemed to be deficient from the start.
Now I'm trying to buy a Pyrocystis fusiformis culture again and found a lab called PyroFarms, but before I order, I wanted to check if anyone had any past experience with them. or alternatively, if you could name any other trustworthy sources where I could buy a P. fusiformis culture, I would be grateful.
I am a student of biotechnology and an independent SARS CoV-2 researcher from India. For finding the academia research status and analysis of the integration of innovation with research, I have created a set of Multiple choice type questions about your experience as a researcher. The google form requires nothing but your honesty and openness for research. Feel free to ask questions and DM. The questions will assist in gauging the level of innovation and writing in academia.
If possible, please do forward this little form to your fellow researchers and other amazing scientists. I would be highly grateful.
Thank you
I am not being unable to isolate DNA from J2 stage nematodes. The nematodes have been extracted from gall and currently are in water. I used extraction methods using sonnicator, glass beads, mortar pestle using liquid nitrogen and even qiagen blood and tissue DNA isolation kit, but no luck so far. I must be doing something wrong somewhere. I will be highly obliged if someone can share a working protocol for DNA isolation from juvenile nematodes or some tips how I can achieve this. Thanks
As all the world is busy in discovering cure for COVID-19, so why not radiation? COVID-19 has been reported to effect the human's lungs and liver (specific tissues), So, what if those parts are exposed to radiation for the rapid recovery of the patients? Is it possible? Or, is there any possibility for radiotherapy of COVID-19?
I am Janu Newar, Ph.D. Scholar from School of Biotechnology, KIIT University, Bhubaneswar, Odisha. Our lab is basically working on proteins from biological sources. This work involves the identification of some proteins. I would like to request some help with the Agilent 6530 Q-TOF instrument.
For identification of the protein, we are using a glass insert of 0.2ml volume (Part no: 5188-5390). My query is can we use 0.025ml of the sample volume in this insert and can inject 0.02ml of sample in the column. If not, then how much lower volume can we use in this 0.2ml insert?
I want to do dialysis purification for the cyclic peptide 2K after its modification using membrane dialysis MWCO 1K but I am worry that the cyclic structure has a smaller size than its linear one and ca pass through the membrane.Is the cyclic structure has bigger or smaller size compared with the linear structure?
Direct experiences with Biotechnological and Biomedical Approach? Therapies, long-term implications?
I'm not looking for definitions but protocols and methods that you personally have made and put into practice. Any information you have is welcome.
By using this cytokine kit I am getting high absorbance value in my positive controls. Recommended values for positive controls are 1.5-2.5, but I am getting values around 3.0-3.5.
In some of my negative control I have absorbance more than the experimental sample. Eg. negative control value 0.04 and sample value 0.01.
I will start an SSF for fish and I want to know what is the best inoculum size to start with ? Is that different from one bacteria strain to another?
I am planning a fluid dynamic experiment and wonder what tubing material is biocompatible for cell culturing experiment. Also wonder if there is a tubing material that can be sterilize.
What are the limitations and disadvantages of Real-Time PCR (RT-PCR)?
What is a more specific and sensitive technique that can be used in the laboratory instead, particularly in cancer diagnosis?
Novozyme 234 is the best enzyme for the isolation of protoplast but in recent days the Novozyme 234 are not commercially available. So is there any other method (enzymatic) for this isolation?
Dear scientists and researchers. I ask those who have knowledge of the names of Free of charge, indexed scientific journals in the field of biotechnology; gives us links to those Journals.
The bio-technologies today are becoming important part of the industry and science research development and innovation. In case of brain tumor or injuries, apart from surgical intervention, the data may also be collected via noninvasive bio-signals such as EEG, transmitted, stored and analysed in e-Health Electronic Health Record. What are future research directions in bio-technology driven bio computing?
Couldn't find any fresh publications explaining this. Also nobody usually says what strand encodes all ORFs of HBV.
Probiotics can be used as dietary supplements or drugs to treat diseases. The genetically modified probiotics will offer much more beneficial features than the wild type probiotics. Does FDA approve genetically modified probiotics? When do you think they will approve? What scientific questions do we need to resolve before FDA can approve? Can we use genetically modified probiotics as dietary supplements without FDA approval?
Is it possible to use Artificial Intelligence (AI) in Biological and Medical Sciences to search databases for potential candidate drugs/genes to solve global problems without first performing animal studies?
Can anyone suggest a method to quantify proteins in melanin containing samples?
Dear all,
I am re-writing now my previous question.
Are you aware if there is any protease with very low efficiency to cleavage IgG ?
Are you aware of any publication?
Regards,
Daniel