Questions related to Color
I wanted to know how will I be able to visualise hydrophobicity and electrostatic potential of proteins from the 3D structure and colour them?
I'm synthesizing a substituted phthalocyanine from phthalonitrile in n-pentanol solvent using DBU as a base and a metal salt but the colour of reaction mass is not turning blue/green after refluxing as reported in the literature. When I try this reaction in a small scale (50 mg), I get a blue/green colour. What went wrong? Now I am only getting a brown colour formation which may due to polymerization or triazine formation of the substrate. What precautions should I take to always get the desired phthalocyanine product?
Is there significant difference between these other element present which highlighted as black? This is from Scanning Electron Microscope-EDS
I am doing coarse-grained MD simulations using the CG-MARTINI forcefield of GROMACS and my system consists of a box with two different peptides in solution. Is there any way in which I can colour these two peptides differently (in order to distinguish between them in the same system clearly) while visualising using the VMD program?
I have cultured PBMC and some RBC contaminant for 2 weeks in suspension and low attachment plate. From the very first day, I noticed that there are many cells that have already turned all black-ish or grey-ish color. I don't know why and I keep continuing my culture process because the sample is very limited and I don't want to waste any samples. This is very confusing as because I will do viability assay using trypan blue, but the cells were already black. So I can't see the blue-ish color that supposed to appear. For additional information, my medium is DMEM F12, EGF, FGF, CoCl2, ITS, Penstrep, and Amphotericin.
I am looking for an implementation of the classic colour Stroop task online so that I can use it as part of a bigger study that I will run in Qualtrics or gorilla (with participants pressing keyboard keys to reply). Gorilla provides a demo, but it is too short and I find it confusing as there is no correspondence between the keys to press and the colours (they use Q for red, W for yellow, O for blue, P for green) and there is no reminder of which key to press for which colour either, so I don’t think it’s ideal.
The one found at the link below works well, but it is a demo only. Plus, I cannot record the data in Qualtrics/data.
Any help would be appreciated!
Thank you,
Dimitra
You are invited to participate in a survey, entitled “Race-based Traumatic Stress and Microaggression in Nursing Faculty of Color.” The study is being conducted by Queyka SaintLouis and Shannon Avery-Desmarais, Department of Adult Nursing of The University of Massachusetts Dartmouth, 285 Old Westport Rd, Dartmouth, MA 02368, 508-910-6598, [email protected] and [email protected].
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Risks to participants are considered minimal. However, if you do feel discomfort, you are encouraged to reach out to your primary care provider. You can call 988 in the event of a mental health emergency. You are also offered to contact the racial equity support line at 503-575-3764. This resource is available Monday through Friday 10 AM to 7 AM Pacific Standard Time, however, you may leave a voicemail should you call outside of regular business hours. https://www.linesforlife.org/get-help-now/services-and-crisis-lines/racial-equity-support-line/ . There will be no costs for participating, nor will you benefit from participating. You will receive a $15 Amazon gift card to thank you for your time. Identification numbers associated with email addresses will be kept during the data collection phase for tracking purposes only. A limited number of research team members will have access to the data during data collection. This information will be stripped from the final dataset.
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Thank you.
when any compound dissolves in another solvent, what are the requirements to say it dissolves ?
Like the solution should be transparent that I know, but what about color. I dissolve a compund A1 composite to KOH, results in colorless and transparent solution. I dissolve A2 composite to KOH, results in yellow coloured transparent solution.
So can I say that A2 is dissolved or not ?
Note : Both A1 and A2 are different composite of a similar element.
I have anti-graffiti surface samples sprayed with graffiti spray. I am going to measure intensity changes in color of surface. How can I quantify them?
learning and treating in our clinic and what is the new method is using
I learned that it is happening because iron and gallic acid are reacting after examining literature. I just want to know if it's normal to happen and if so, how to fix this issue to achieve the best results possible.
Dear all researchers,
I have gone through many articles about Traction Force Microscopy, however, I wonder if anyone could provide me with some advice regarding how to change the representative colours of the force, instead of rainbow colours but colours like the attached image?
I am conducting the IHC experiment with cultured cells.
However, I experienced the non-specific staining with all my samples.
The nucleus were stained by DAPI, but beside the blue of DAPI, they were also stained by the conjugated red-fluorescent dye of secondary antibody. I noticed that the cell nucleus was stained even much more strongly red than the other target parts. So when I merged two color images together, the red and blue signals overlapped, causing them to turn purple-pink. How to remove non-specific red staining of cell nucleus?
I put my image and our protocol below.
1. Fixation step: 4% Paraformaldehyde (PFA) 2ml, 20 min, Room Temp. --> 1. Washing with PBS (shaking) (3 times)
2. Permeation: 0.1% Triton in PBS for 10 min --> Washing with PBS (5min)
3. Blocking: 10% Serum in PBS, 1.5h
4. Primary antibody: Remove blocking solution, not washing --> Incubate cells in working solution of (0.1% Triton+10% Blocking Serum + Primary Antibody)/PBS (overnight, 4oC) (ratio 1:500)
5. Secondary antibody: Remove Primary antibody, wash 4 times with PBS --> Incubate cells in working solution of (0.1% Triton+10% Blocking Serum + Cy3-conjugated-Secondary Antibody (ratio 1:500) + DAPI (ratio 1:1000))
--> Washing 3 times with PBS
Hello, I wonder about calculating the extinction coefficient of colorful solutions.
I measured the absorbance of some solutions with a UV-vis spectrophotometer (Left).
Using two relations, I calculated the absorption coefficient (α) and extinction coefficient (k).
The final k values of solutions I calculated are about 10^-4 ~10^-5.
Are the k values reasonable considering the solution colors in the optical images?
I'm not sure whether the k values should be higher.
Please give me some advice if you have any ideas.
Thank you.
I am planning to study adsorption efficiency of dye at different pH. As we know, some dyes would change colour at different pH. Therefore, is it necessary to plot different calibration curve at each pH? 7 different pH with 4 different dye concentrations would mean 28 solutions that have to be made. Is this a common approach?
With increasing sorbent dose in an adsorption test for heavy metal removal , there were various shade of decolourization in metal solution after adsorption observed. Does this colour change have any effect on sorption capacity .
I am trying to express a FAD containing enzyme of mycobacteria in E.coli. I am able to purify the protein which is slightly yellow in color but it seems that my FAD is all unbound to the protein. How can I express the protein which has tightly bound FAD?
If an unknown powder or solid sample has given a yellow color result, what INORGNIC or METALLIC COMPOUNDS ARE INDICATED. I am not interested in any controlled organic substances , only inorganic substances. Any shade of yellow qualifies. TY.
Greetings to all,
As part of my research, I am synthesising carbon quantum dots. Using ethanol as the solvent, I performed a solvothermal method. As I have tried different temperatures and times (up to 70 hours of treatment, the color remains yellowish), as well as catalysts (Con. Sulfuric acid, Con. Nitric acid, and Con. Hydrochloric acid - Nitric acid does not give fluorescence). In spite of this, I was only able to obtain yellowish green fluorescent. It shows a very very light blue color without a catalyst (nearly imperceptible). If you could please assist me in figuring this out and tell me any important points I need to keep in mind while synthesizing carbon quantum dots. The precursor used in this process is resorcinol.
I'm using the vanillin-sulphuric acid method to cuantify saponins but the colour that I observe is yellow instead of red-purple.
The method I follow from the literature is:
- 1 ml of sample extract + 0.25 ml 8% (v/v) vanillin in methanol + 2.5 ml 72% H2SO4
- Incubate mixture in 70ºC water bath for 15 min and then in ice for 5 min.
- Read absorbance at 560 nm.
Any sugestion or solution for this problem?
Thanks!
Garret, 2020 used traps of blue, yellow, and white colors for sampling and collected several specimens.
I'm wondering narrow bandgap-color of red wavelength is almost same with powder and solvent when illuminated.
However, when the qd bandgap become widen, then powder color and illumination color is not match. Why this phenomenon happens?
Qupath software is the stereology
I am working with 5-20 nm gold nanoparticles in order to conjugate them with IgG. when I try to change the buffer of particle to the appropriate buffer samples aggregate in centrifugation step. so, I did titration step for selecting the optimum concentration of IgG without changing buffer but when I put conjugated samples into the centrifuge the aggregation happens again. i should mention that before centrifuge there is no change in the sample color and also after it, but a significant black spot precipitate in the bottom of micro-tubes. I tried different pHs: 7-9.5 but the same results.
I would be appreciated I you provided me with some guides and advices.
Is there a method to plot flower color reflectance spectrum on a butterfly visual system (over six photo-receptors),like what was usually done to bees (a hexagon) and flies (a square)?
The visual system in R package pavo does not have in-built butterfly visual system. The manual showed how to construct mantis shrimp (with ten photo-receptors) visual system by ourselves, but it seems impossible to use the constructed visual system into the analysis.
(usually the system is used by the code "visual=apis" for bees, "visual=musca" for flies, etc.)
Why are we observing a black color on the surface when using the nano Bubbler in the aeration tank?
I use BG 11+ Media but with an alternative trace metal mix.
Ecoplate is done to know the metabolic diversity of soil at community level.
Hello, I am currently doing a research about essential oil extracted using soxhlet extraction from guava (Psidium guajava L.) and my solvent is n-hexane. I am just wondering what should be the color of the solution after rotary evaporator? Because currently, we have a crude like greenish color.
I have a fluorescent compound, I want to represent the colors using CIE diagram. But, I'm confused how to do. Please help me with references or examples.
Hello All,
I have been using the Albumin standard ampules from thermo scientific of 1ml to make a standard curve for protein and I even got a nice standard curve.
But when I am using the solid BSA flakes from Sigma Aldrich to prepare the solution required and when I am doing the Bradford assay with this sample, the colour is not changing or can say the Bradford reagent is not working on this solid BSA sample to detect the protein.
The ampules are of 2mg/ml in a 0.9% aqueous NaCl solution containing sodium azide. But the solid BSA flakes from Sigma Aldrich is a pure protein.
So, is this the reason for not detecting the protein through Bradford reagent on solid BSA flakes?
If anyone encountered the same issue please let me know.
And also, if some one has a solution to solve this problem, I would really appreciate it.
Thankyou,
Navneet Kallapalli
Why after the adsorption of methyl orange dye by my adsorbent, in addition to the color peak in 464 nm, another peak has appeared in 372nm n in theuv-vis spectrum. It should be noted that this additional peak can be seen only in low adsorbent dosage, higher color concentration and shorter conatct time!
thanks in advance for your help
Does anyone have good lab practices or experience of growing primary cells without antibiotics and anti-fungal?
Recently I ordered primary airway epithelial cells (adherent) from ATCC. They provided Anti-anti (penicillin + amphotericin B solution) along with basal media. so I prepared media with anti-anti solution and re suspend cells. so I observed cells were not attaching for 2 days and 3rd day there was contamination without change of media color. it was rod shaped bacteria with cells when i saw under microscope.
So when I complaint to ATCC, they suggested me some points. One of them was not to use anti-anti solution (antibiotics), as it can cause stress to primary cells.
Now I am planning to grow primary cells without adding antibiotics, But I am scared that there will be contamination and I dont want to lose cells (I have only single vial).
Any suggestions?
I used miltenyi whole blood CD3 micro beads to separate the T cells from whole human blood using the automacs seperator. Usually after the separation is done by the machine, there is a clear T cell isolate. Yesterday I ran the machine twice and the isolate had brown coloured sediments at the bottom both the times—probably micro beads. I have been using the same settings for a long period with no issues. Has anyone encountered this before and can someone please help troubleshoot? is this an issue with the beads or the column or something with the setting?
I want to draw cassini oval parametrically with using analytical face option of cst.
I write python code below. If any one can help me ı will really appreciate.
import numpy as np
import matplotlib.pyplot as plt
def cassini_oval(x, y, x1, y1, x2, y2, a):
# Compute the Cassini oval equation
return np.sqrt((y - y1)**2 + (x - x1)**2) * np.sqrt((y - y2)**2 + (x - x2)**2) - a
# Parameters
x1 = -15.0 # x-coordinate of the first focus
y1 = 0.0 # y-coordinate of the first focus
x2 = -x1 # x-coordinate of the second focus
y2 = 0.0 # y-coordinate of the second focus
a = x1**2 # constant 'a' in the equation
# Generate points for plotting
x_range = np.linspace(-30, 30, 1000)
y_range = np.linspace(-30, 30, 1000)
X, Y = np.meshgrid(x_range, y_range)
Z = cassini_oval(X, Y, x1, y1, x2, y2, a)
# Plot Cassini oval
plt.figure()
plt.contour(X, Y, Z, levels=[0], colors='b')
plt.xlabel('x')
plt.ylabel('y')
plt.title('Cassini Oval')
plt.axis('equal')
plt.grid(True)
plt.show()
I would like to add different colors in a vertical pattern to the plot region of the forest plot to define the effect size magnitude. How could I do that?
Why does the color of the reactant change depending on the temperature during radical polymerization? Although it was set to the same temperature, it is expected that there would be a slight temperature difference. The color of the reactant at higher temperature appeared darker.
I am doing Land Use Land Cover Classification in GEE using Landsat 8 Level 2, Collection 2, Tier 1 dataset. While collecting training points in the composite image, The challenge I am encountering is that urban and barren land areas appear similarly in the color composites I've tried, which were based on combinations of bands 5, 4, 3 and 7, 6, 4.
What genus of bacteria can grow in YDCA medium and What color is the colony of these bacteria when grown on YDCA medium ?
Hello, I have nylon fabric dyed with Acid dye. I need to prove for part of my project, is it possible or not possible, the reaction between dye molecules absorbed in nylon fabric with water molecules when the fabric is immersed in water. I hope someone helps me to find the best answer to this question.
Hello. Have you ever observed calcofluor white causing a color change to purple-pink in colonies of Candida albicans?
i have been performing quantitative phytochemical analysis for past week and have found it really difficult to understand how to set a specific concentration of sample for conducting the experiments. i have been thinking about taking very low concentrations of sample like 10-50 micro liters. but here the practical difficulty is that most of the time color is not getting developed for specific tests and it is difficult to take OD value in spectrophotometer. what to do?
Hello dear experts,
I have four solid samples of a mineral. With my naked eye, I can see clearly that they have distinguishable colors. I already have UV-Vis spectra (reflectance and absorbance) ranging from 200 to 1000 nm for each of these samples. I need to know can I determine the color of each of these samples using this UV-Vis spectroscopy and how can I do that?
Please kindly advise me.
Thanks.
i have been performing quantitative phytochemical test for flavonoid detection of my plant sample for past 4 days. i have been using the 10-50 concentrations of sample and still not getting any color reaction in my sample. i tested the quality of chemicals thrice and still there is no color development. what should i do?
The photocatalysis was done using nano zinc oxide coated activated carbon at neutral pH using 0.3g of catalyst. Eventhough the solution doesnt show full decolourisation(it shows pale blue colour) the absorbance value is shown as 0 after 45 minutes of treatment. The initial concentration was 50 mg/l and dilution factor of 20 was adopted.Since the solution is still coloured can this value be reported as 100% degradation?
while doing column chromatography to extract glycosides with 1:2 ratio of ethyl acetate and petroleum ether I got elution's. based on color two of green and yellow elution's tested positive for sterols. is both are same or different sterols?
The color of dental resin cement with amine and phosphonate will change during curing process.
When a flask of soil is connection to 0.01N NaOH to capture CO2, the naoh is used in order to capture the CO2.
However, when fresh solution of NaOH is directly titrated with dil.Hcl using phenolphthalein indicator, still the colour change is being observed. What is the reason for this?
Hello Everyone, may I ask one question.
Caco2 cells were culture on hydrogel with different concentration. Color changes of medium was detected on some well after 3 weeks of cell culture. Can someone please give me a clue of what could be the main possibility?
When performing a thin layer chromatography analysis of an aqueous plant extract, violet spots corresponding to the amino acid standards used to study their presence appeared, and very faint blue spots appeared in place of the samples after using ninhydrin reagent. These faint blue spots do not correspond to any of the more than 20 amino acid standards used as they showed defined spots of intense violet color. What interference in the sample could have caused these faint blue spots when reacting with ninhydrin?
A solvent system of Chloroform: methanol: 25% ammonia (40:40:20) was used and the blue spots presented an Rf (0.57).
The cells do not confluency after thawing. Even after 10 days, the color of the medium does not change. I change the medium, the non-adherent cells disappear and then the density in the flask decreases. It has been going on like this for 3 months. Is this an adaptation problem?
Hi,
I came across an issue when graphical results do not match the optimal reconstruction in the list section in RASP v4.0. In the graphical section, I would have 50:50 division of a nod pie, color coded as 50 state B : 50 state G. But in the List section the same nod gives node 66: F 98,48 C 0,51 G 0,51 B 0,51 BE 0,00 BEF 0,00 ....
Meaning I would expect the graphical result to be pie of the majority of F state.
Any idea why that could be?
Thanks,
Karolina
Zhuangzi lived around the 4th century BCE during the Warring States period. His work, also titled "Zhuangzi," is a foundational text of Daoism (Taoism) and is known for its philosophical depth, humor, and literary style.
Daoist Philosophy
The Dao (or Tao) is a central concept in Chinese philosophy, particularly in Daoism (Taoism). It's a fundamental idea that underlies the nature of reality, existence, and the way one should live. The term "Dao" itself translates to "the Way" or "the Path." Here are key aspects of the Dao:
- Unnameable and Ineffable: The Dao is often described as unnameable and ineffable. It transcends human language and understanding. In the classic Daoist text, the "Dao De Jing" attributed to Laozi, it is said, "The Dao that can be told is not the eternal Dao; the name that can be named is not the eternal name."
- Unity and Oneness: The Dao represents the underlying unity and oneness of the universe. It is the source and essence of all things, connecting everything in existence. Daoism emphasizes the interconnectedness of all phenomena.
- Natural Order: The Dao is associated with the natural order of the universe. It is the way things naturally are, beyond human attempts to impose artificial structures. Living in harmony with the Dao involves aligning oneself with the natural course of events.
- Wu Wei (無為) - Non-Action or Effortless Action: Daoism advocates the principle of Wu Wei, which is often translated as "non-action" or "effortless action." It doesn't mean complete inactivity but rather acting in accordance with the natural flow of the Dao, without unnecessary interference or resistance.
- Balance and Harmony: The Dao emphasizes balance and harmony. It is neither extreme nor excessive. Living in accordance with the Dao involves finding a middle way, recognizing the interplay of opposites, and avoiding extremes.
- Spontaneity and Simplicity: The Dao is spontaneous and simple. It operates without deliberate planning or artificial complexity. Daoist philosophy encourages a return to simplicity and a natural way of being.
- Eternal and Ever-Changing: The Dao is considered eternal and ever-changing. It is a paradoxical concept that transcends time and yet is in constant flux. It is both timeless and continuously evolving.
- Intuitive Understanding: Daoist wisdom is often characterized by an intuitive understanding of the Dao. It is not necessarily something that can be grasped through intellectual analysis but is recognized through direct experience and insight.
- Transcending Dualities: The Dao transcends dualities such as good and bad, beautiful and ugly, success and failure. It encompasses the totality of existence, recognizing the relativity and interconnectedness of opposites.
The Challenges of Interpreting & Translating Zhuangzi
Interpreting Zhuangzi poses several challenges, and the limits of translation play a crucial role in this process. Here are some aspects to consider:
- Cultural and Linguistic Differences: Zhuangzi's ideas are deeply rooted in the Chinese language and cultural context of his time. Translating these ideas into another language, especially one with different philosophical traditions, can lead to misunderstandings or loss of nuance.
- Conceptual Nuances: Certain Chinese philosophical concepts may not have direct equivalents in other languages. Translators often face challenges in conveying the subtle nuances of Zhuangzi's thought, such as the Dao (Tao), which encompasses the idea of the Way or the natural order.
- Ambiguity and Paradox: Zhuangzi is known for his use of paradox and ambiguity. Translating such literary and philosophical devices can be challenging because the meaning may shift or become less apparent in another language. Maintaining the richness of his language is a formidable task.
- Cultural References and Allusions: Zhuangzi often used anecdotes, allegories, and historical references that may be unfamiliar to readers from different cultural backgrounds. Translators need to decide how much contextual information to provide without overwhelming the reader.
- Poetic and Literary Style: Zhuangzi's writing is characterized by a poetic and literary style. The beauty and artistry of his prose may be difficult to capture fully in translation. The rhythm, wordplay, and rhetorical devices may not carry over seamlessly.
- Interpretation of Daoism: Daoism, as presented by Zhuangzi, involves a way of thinking and living that may be unfamiliar to Western philosophical traditions. Translators must carefully choose words and concepts that convey the essence of Daoism without imposing foreign philosophical frameworks.
- Different Editions and Manuscripts: The Zhuangzi has different editions and manuscripts, which can vary in content and arrangement. Translators may need to make choices about which version to use and how to reconcile differences.
Given these challenges, scholars and translators often provide extensive commentary and annotations alongside translations to offer readers a deeper understanding of Zhuangzi's text. Multiple translations by different scholars can also be valuable for gaining a more comprehensive view of Zhuangzi's ideas, as each translator may emphasize different aspects based on their interpretatio
On Stillness and Adaptability:
聖人之靜也非以不動為靜，寂然和之。
Shèng rén zhī jìng yě fēi yǐ bù dòng wéi jìng, jì rán hé zhī.
Translation: "The stillness of the sage is not attained by immobility; it is achieved through tranquil harmony."
聖人之樂水也，聖人之樂山也；聖人之動也，聖人之靜也。
Shèng rén zhī lè shuǐ yě, shèng rén zhī lè shān yě; shèng rén zhī dòng yě, shèng rén zhī jìng yě.
Translation: "The sage finds joy in water, the sage finds joy in mountains; the sage's movement is joyful, the sage's stillness is tranquil."
On Trained Spontaneity and Agile Decision-Making:
射猛於飛鏑者，禪讀之人也。鏑心見於物而不見於己，已物與己反而不知不知之知。
Shè měng yú fēi zhú zhě, chán dú zhī rén yě. Zhú xīn jiàn yú wù ér bù jiàn yú jǐ, yǐ wù yǔ jǐ fǎn ér bù zhī bù zhī zhī.
Translation: "The archer who shoots fiercely with flying arrows is a person of Zen reading. The arrow's heart is seen in the target but not in oneself, understanding is turned toward the object and oneself, not knowing this knowing."
耳任聲以聞，眼任色以視，心任意以思，體任勞以行。"
Pinyin: "Ěr rèn shēng yǐ wén, yǎn rèn sè yǐ shì, xīn rèn yì yǐ sī, tǐ rèn láo yǐ xíng."
Translation: "The ears are open to sound, the eyes are open to color, the mind is open to thought, the body is open to labor."
On Wu Wei and Effortless Action:
道不可道，名不可名。道名始離
Dào bù kě dào, míng bù kě míng. Dào míng shǐ lí.
Translation: "The Dao that can be told is not the eternal Dao; the name that can be named is not the eternal name."
行無行，名無名。事無事，名無名
Xíng wú xíng, míng wú míng. Shì wú shì, míng wú míng.
Translation: "The way that can be walked is not the eternal way; the name that can be named is not the eternal name."
On Technology & Meaning:
魚罾之乎者也，莫之以其魚；麗兔之乎者也，莫之以其兔；白燕之乎者也，莫之以其燕。言之隨也，莫之以其義；故曰，失之者，可勿捨乎？
Yú zēng zhī hū zhě yě, mò zhī yǐ qí yú; lì tù zhī hū zhě yě, mò zhī yǐ qí tù; bái yàn zhī hū zhě yě, mò zhī yǐ qí yàn. Yán zhī suí yě, mò zhī yǐ qí yì; gù yuē, shī zhī zhě, kě wù shě hū?
Translation: "The fish trap exists because of the fish; once you've gotten the fish, you can forget the trap. The rabbit snare exists because of the rabbit; once you've gotten the rabbit, you can forget the snare. Words exist because of meaning; once you've gotten the meaning, you can forget the words."
Since there are so many layers to interpreting Chinese, I will try to look at the meaning of each character used in Zhuangzi´s writings. Classical Chinese, in the way I was taught, is a reading of character by character.
Hello ResearchGate Community,
I am searching for a water-soluble dye that can withstand a prolonged period (one month) at 85°C and 5000 psi. It's crucial that the dye remains stable and retains its color across both acidic and basic pH environments. The dye's ability to maintain consistent color under these extreme conditions is essential for my research.
I would appreciate any recommendations for dyes known for their stability and color consistency in such settings.
Thank you in advance for your insights!
Best,
Musa
Hello. I have an ArcMap file which has a layer with my study area and a numbered grid overlayed on it. Both are in the same layer. I wish to colour code each cell on the basis of the built up area percentage of that cell. I want the end product to look like a mosaic of 3 different colours, since each cell would be coloured with one of 3 colours (to be chosen by me). Could anyone tell me how to do this?
Thanks in advance!
Unclear, what you mean when you say you have two layers (study area and the overlaid grid layer ) and then that both are in a single layer. Do you mean there is a layer 'group' that has the two layers in it? ( it always really useful to provide and attach screen captures, not only the geometry, but also the 'properties' of the layers, see https://desktop.arcgis.com/en/arcmap/latest/map/working-with-layers/setting-layer-properties.htm ). ... https://desktop.arcgis.com/en/arcmap/latest/map/working-with-layers/a-quick-tour-of-map-layers.htm#ESRI_SECTION1_46AC183DD2614893820C4469453CBD7D
As a note, 'mosaic' has a specific meaning, for instance when stitching together many overlapping remote sensing images ( see https://desktop.arcgis.com/en/arcmap/latest/tools/data-management-toolbox/mosaic.htm ), and I am going to guess it doesn't apply to this situation.
The other assumption is that the numbers in the 'numbered grid' ( all rasters / grids have all sorts of numbers associated with them, like row / column numbers, etc.) are 'percentages' ( floating point or integers? ).
There are two basic approaches, the first is to change the symbology ( see https://desktop.arcgis.com/en/arcmap/latest/map/working-with-layers/a-quick-tour-of-displaying-layers.htm ) and is similar to https://support.esri.com/en-us/knowledge-base/how-to-apply-the-same-symbology-to-multiple-rasters-in-000014408
The second is to 'reclassify' the data into a new layer ( https://desktop.arcgis.com/en/arcmap/latest/tools/spatial-analyst-toolbox/an-overview-of-the-reclass-tools.htm ) and use that for display.
You don't mention what specific version of 'ArcMap' you are using, etc. or how familiar you are with ArcGIS in general.
I need to know how the colored proteins react with BCA to give the appropriate color which is to be read by the spectrophotometer? I mean I have worked with colorless proteins up till now. How to go about with these colored protein estimation?
The attached file shows that the results contain different isotherms and colors indicating different temperatures, but on the scale all colors indicate the same temperature value. What could be the error?
I have set several layers of material with different absorption coefficients and I want to look at the temperature distribution when the beam falls on the end (file Неоднородный наоборот)
In the case of a constant absorption coefficient in the entire volume, the program produces logical results with different temperatures (file Однородный)
Suppose the Earth is a black body covered with a layer of plastic; it is then quite normal that the incoming radiation is completely absorbed and transformed into heat. But, the reality on Earth is that there are percentages of blue (seas and oceans), green (forests and agricultural lands), yellow (desert or Sahara), red and brown (lands), etc.
Each color absorbs a particular radiation percentage, different from other colors and lower than the percentage of the radiation absorbed by a black body. Suppose we replace areas on Earth of different colors with black solar panels, whether photovoltaic or thermal. In this case, they will undoubtedly absorb much more energy and transform it into thermal energy, contributing to global warming.
Hi, can anyone give suggestion how exactly important procedure to synthesis graphitic carbon nitride from urea? I had synthesized using urea with 550 degree Celcius 4 hours 2 degree per minutes. However, the powder is brown color instead of light yellow. please help me. Thank you.
Hi, everyone!
Recently, i want to synthesize perovskite QDs by hot injection reported by prof kovalenko (Ref: 10.1021/nl5048779). i find the color PbBr2 precursor (PbBr2 + OA +OAm in ODE) solution was yellow when i heat at 120℃, and the color becomes brown at 150℃.
Is this normal? i hope you can give me some advice, thank you!
I make a masterbatch by mixing polyethylene and zeolite 4a with different concentrations and using a typical industrial extruder machine. Sometimes the extruded granules change color to orange about an hour after being extruded. I can not find a reason why.
other methods apart from commutative algebra, minimum number of colors, relationship between ideals with edges and nodes,
Hello everyone,
I'm currently working with TLC plates stained with NP/PEG and I've noticed distinct yellow and green bands, indicating the presence of flavonoids. I understand that both colors generally signify the presence of flavonoids, but I'm curious if there is any information on whether the green bands might be associated with a specific type of flavonoid, and similarly, if the yellow bands correspond to another type of flavonoid.
Any insights or references on this matter would be greatly appreciated! Thanks in advance.
Hello all dear
I need a help
Thanks in advance
As 2D material are polymorphic in nature and through mechanical exfoliation these different structure of same material is studied. and based on optical images how we can identify different structure that is based on colour contrast ?
These are colonies that I isolated from a product. I have found difficulties in identifying the name of the microorganism by observing its morphology. Could someone tell me what microorganism it is by examining the macroscopic morphology?
when we do synthesis of nanoparticle through Green route, we always notice the initial color change by adding Plant Extract and the Salt like Zinc acetate in ZnO NPs etc. and if the color change occur we say our NPs synthesized. so what's the logic behind this, why the color change occurred.
Is it by using the inhibition (%) formula or simply plot a standard curve using absorbance and concentrations?
If using the inhibition (%) formula,
Is it correct if the negative control color is yellow-orange while with the increasing concentration the more intense the color of blue/purple? blank: ethanol. I used Trolox for standard
Is there any one who can guide me that why Blood has red colour?
I am culturing Human Aortic Endothelial Cells from ThermoFisher (Cat #: C0065C). I am doing it with the media and supplements they sell for it, which are called M200 and LVES respectively. Despite the version of the media I am using containing phenol red, I have never seen cell media change color. Although they do not change color, I very often observe round, refractive particles that look really similar to cells when I add trypsin to them which leads me to believe these particles are unattached cells. I am guessing these unattached cells are dead cells, but I am not sure why they are dying. If the media was exhausted, there would be a shift in pH and thus in color, right? If there was not meant to be any shift in color with these cells I do not see why the company would sell a version of this media with phenol red in it.
I have been testing different times to change media, but it seems to fluctuate. Sometimes they will last three days and still be fine, but sometimes I see those refractive particles just two days after I have changed media.
Any suggestions? Perhaps this is normal and nothing to worry about.
When I conduce an esterification reaction using oleic acid P.A, occurs a variation in color of final product generated (liquid). Is this color due occurence of some concurrent reactions generating other products in medium? Which are the majority products in this reaction that causes this phenomenon?
Has anyone used FRITSCH ANALYSETTE 22 NeXT Nano for green synthesized silver nanoparticles?
I am having trouble setting the parameters (%pump, beam obscuration, absorption index, etc) to get accurate beam obscuration and readings. I am working with solutions prepared with plant extracts and AgNO3 solutions, the UV spectra make us believe that we have good synthesis since characteristics maximums appear in the range of 420-470 nm, and a change of colours is also observed. However, when using the Fritsh equipment we are not getting a signal. At the moment we do not have other equipment to measure.
Is that a chemical issue or atmospheric change? Kindly give some tips...
Thank you
Hi friends,
I have a question about my fibroblasts cell culture. Lately they have been looking different than usual. As you can see in the photo (second one), the cytoplasm appears to be much more granular and the mitochondria less visible, compared to the photo opposite. Culture medium has correct color, cells grow as always.
Is this normal, or is it a sign of a cell culture infection?
I have synthesized Fe3Se2CO9 by modified Hieber synthesis method...But I am getting a green colour cluster along with the red coloured Fe3Se2CO9 clusters...This is minimizing the yield..How to avoid it??
I am getting only three colors blue, green and red. so sometimes it become difficult to identify. How can we change this default condition?
Hello everyone,
I am examining the activity of immobilised peroxidase using the ABTS assay, with varying concentrations of ABTS, keeping every other factor stable. I noticed that for low initial concentration of ABTS (0.05-0.1mM final C in assay), I initially get a green-coloured product (the radical), which over time (t>48h) it gradually gets pink. My system's conditions are pH 4, 0.1M phosphate-citrate buffer, 0.44mM peroxide and peroxidase immobilised on a silica support. The pink colour is NOT observed for higher concentration of ABTS, for the free enzyme or for control samples containing only the silica support. By observation, I figured that initially the silica-peroxidase nanoparticles turn pink and then the assay is slowly turning pink (observations over days). Could someone please explain why this might be happening?
Thank you :)
I extraction RNA from Physcomitrella patens (Moss) Following the protocol of RNAiso Plus. Firstly using a tissue lyser and added 1 ml RNAiso plus and vortex. next step Add the chloroform after centrifuge I separated the top liquid layer but the problem is yellow color liquid. I could not avoid the yellow color at ned of the extraction process after the precipitated pellet is brown.
Which colors of light are most able to penetrate deep into the ocean and role of forest in maintaining the balance between oxygen and carbon dioxide in the atmosphere?
I immobilized the laccase enzyme with glutaraldehyde on silica airgel support. The protein binds to the support, but its activity is quite low. The color change takes place very little in the reaction liquid, but the color of the silica changes very much. How can I solve this?
Currently conducting alpha glucosidase inhibition for my test compound. Both the enzyme and substrate concentration was optimised at using 0.1 M of phosphate buffer at pH 6.8.
When I perform the assay with the test compound,the negative control (enzyme & substrate) reaction shows lesser absorbance compared to test wells in 96 microtitre. In addition the change is colour is is very pale to not obvious change in colour even after 35 minutes of reactivity observation.
The substrate too do not dissolve well in the buffer solution. It appear clear with noticeable crystalline residues.
Would be very helpful if the expert in this field could advise as to what could be the solution in this situation?
I purified my protein of interest using Ni-NTA chromatography. The protein was eluted but it eluted with a yellowish brown color which I have experienced before. I believe it was due to excess DTT (buffer mismatch) which led to leaching of Ni with my eluted protein.
Any ideas as to how to remedy this and remove the color (and Ni) without compromising the integrity of the protein.
Thank you.
living in a place with low gravity spot (9,375), probably due to an archaic force 9+ earthquake 500.000 years ago (only perceptible through ESA satellite, see photo), Formentera island, in Balearic archipelago, Spain ((PDF) (PDF) FORMENTERA WITH LESS GRAVITY (researchgate.net), and following with friends, music and flowers, the holy sunset tradition, that last longer in this part of the island. Sometimes, color red even performs an unusual comeback after violet. So, I wondered if light and spectrum's colors could be influenced by gravity. And of course, they are Why Is Light Affected By Gravity? (forbes.com) . Now, I dream of making an artistic movie on Formentera's sunsets, with solid and revolutionnary scientific bases. What are you thoughts about my crazy idea?
With the development of technology and the development of the market and human attitudes, there is nevertheless racism, whether for color, race, sect, or religion.
I evaluated the antioxydant of ethanolic extract by DPPH. I chose a concentration of 1000 ug/ml and then 2000 ug/ml very high concentractions but no yellow color appeared when adding DPPH. Knowing that my extract is high in polyphenols and flavonoids. Can anyone please expert on this field help me and give me an explanation on what happens?
Thank you
I used two conditions. In one well I stained the cells using only Calcein AM & Hoechst and the other well was stained with Ethidium Homodimer-1 and hoechst.
The cells stained with only Calcein-AM & Hoechst is showing signal in red and the cells which were stained only with Ethidium Homodimer-1 and hoechst showed signal in green color. I am confused now. Why will they signal in those color even when the stains are not added?
I use different types of CL to characterize minerals and materials, like CL color imaging on a light microscope or hyperspectral CL on SEM/EPMA.
Is there any solution to convert a CL spectrum (wavelength vs intensity) to coordinates in a RGB color space to determine the perceived color, in order to be able to correlate and analyze the information coming from the different modes of observation ?
Thank you for your help
I wish to understand color image processing based on quaternion Fourier transform. Where will I get basics of that? Is there anyone working on this topic in India?
Under these plants grow loose mushrooms that in Congolese villages do not consume as their meats change color to the manipulation and to the cut.
It could be a Phlebopus sudanicus or a P.portentosus (which is Asian) or another Phlebopus still unknow
The plant seems to me to be a species of acacia but I do not know exactly the Congolese or African name and the Latin name.
No Congolese University has answered me.
Thanks.
I am working on nanoparticles i need the size of Fe3O4 nanoparticles below 10nm I am using sodium citrate as a stabiliser. In one case I use NH4OH in solution the color of the solution turn black but in the cetrufuge, the nanoparticles are not collected. my teacher said if the color turn to black then nanoparticle absolutely formed find a way to sit down. the method i am using is co-precipetation.
