Fixatives - Science topic

I'm trying to install packages for my masters thesis. When I try to install the package 'foreign' it works but when I try to run it, I receive the message:

Error in .helpForCall(topicExpr, parent.frame()) :

no methods for ‘foreign’ and no documentation for it as a function

How do I fix this? I have R version 4.4.0 on Mac OS 11 or 12 I believe.

Please send me pictures explaining how to simulate buckling of shear stresses in Abaqus 3D with hinged and fixed support ?

The wave function of quantum mechanics at a fixed point in space-time is simply a set of complex numbers. How does the set of these numbers relate to the geometry of the universe?

Please ask if you need additional information.

Hi everyone,

I want to Apply Tests for Special Causes in p-chart, but the sample size for each group is different. So I don’t have a fixed UCL and LCL. Just a question can I apply Tests for Special Causes? Can I calculate index when I don’t have a fixed LCL and UCL.

Thanks,

Hi everyone, I'm trying to extract fungal DNA from wheat common bunt using chelex100 and I've tried several different protocols without any success. I've used different concentrations of chelex100 (5%, 10%, 20%) with different ways of heating (heat block, water bath, boiling water) and different timings (2x 15 mins, 1x 30 mins plus 1x 15 mins, 2x 20 mins). Only 2 things were fixed in all my tries, 1st thing is that I dissolved chelex100 in double distilled injection water and 2nd thing is that I centrifuged the complex at 13000 rpm for 1 min every time it needed centrifugation. I'm not sure what am doing wrong, Like should I try dissolving chelex100 in any specific buffer or something? or should I change my centrifugation speed and time? or do I need to add anything to the complex in different steps?

I'd appreciate if you take the time and kindly answer my question.

Hi users,  I want to create an itp file for my ligand (ligand with ruthenium) complex, im using MCPB.Py for generating itp for my structure during the process i got the following error kindly please help. MCPB.py -i dna.in -s 3 The input file you are using is : dna.in The following is the input variable you have: The variable ion_ids is :  [1201] The variable ion_info is :  [] The variable ion_mol2files is :  ['Ru.mol2'] The variable original_pdb is :  complex_fixed_h.pdb The variable add_bonded_pairs is :  [] The variable add_redcrd is :  0 The variable additional_resids is :  [] The variable anglefc_avg is :  0 The variable bondfc_avg is :  0 The variable chgfix_resids is :  [] The variable cut_off is :  2.8 The variable force_field is :  ff19SB The variable frcmod_files is :  ['LIG.frcmod'] The variable gaff is :  1 The variable group_name is :  dna The variable ion_paraset is :  12_6 (Only for the ions using the nonbonded model). The variable large_opt is :  1 The variable lgmodel_chg is :  -99 The variable lgmodel_spin is :  -99              -99 means program will assign a charge automatically. The variable naa_mol2files is :  ['LIG.mol2'] The variable scale_factor is :  1.0              ATTENTION: This is the scale factor of frequency. The              force constants will be scaled by multiplying the square              of scale_factor. The variable smmodel_chg is :  -99 The variable smmodel_spin is :  -99              -99 means program will assign a charge automatically. The variable software_version is :  gau The variable sqm_opt is :  0 The variable water_model is :  OPC The variable xstru is :  0 ****************************************************************** *                                                                * *======================RESP Charge fitting=======================* *                                                                * ****************************************************************** ***Generating the 1st stage resp charge fitting input file... ***Generating the 2nd stage resp charge fitting input file... ***Doing the RESP charge fiting... =========================Checking models========================== ***Check the large model... Good. The charges and atom numbers are match for the large model. Good. There are 27 atoms in the large model. ***Check the standard model... Traceback (most recent call last):   File "/home/saranya/amber20/bin/MCPB.py", line 692, in <module>     resp_fitting(stpdbf, lgpdbf, stfpf, lgfpf, mklogf, ionids, ff_choice,   File "/home/saranya/amber20/lib/python3.9/site-packages/pymsmt/mcpb/resp_fitting.py", line 521, in resp_fitting     raise pymsmtError('Error: the charges and atom numbers are mismatch ' pymsmt.exp.pymsmtError: Error: the charges and atom numbers are mismatch for the standard model!

Hello,

I am planning to conduct an experiment to identify bacteria bound to IgG by flow cytometry. I aim to focus on live bacteria, so I intend to use the LIVE/DEAD BacLight Kit (Syto9 and propidium iodide) to confirm I'm examining live bacteria. Since I need to fix the samples before acquisition on the flow cytometer, my questions are:

Thanks in advance,

How to fix the mentioned issue in Material Studio Software?

Hello Everyone,

It is part of the dogma of verbotonalism that if you fix the intonation of an L2 you also automatically fix the pronunciation of all/most/many individual sounds. While I think from observation and results reported over the years in many places that this is true, I still have a question.

Has any study been done *with hard statistical data* to demonstrate that this is so? I am looking for something other than impressionistic data. And if such studies exist, please provide the citation if possible. I have no memory of such studies.

Since the Revue de Phonétique Appliquée disappeared a huge amount of publications on VT has also disappeared. I will try a closer look at Govor but I thought I would ask anyway.

Thank you!

Andrew

Hello sir,

I want to apply uplift pressure at the dam base in a gravity dam structure with a fixed base case.

I created step- Gravity - Hydrostatic - Uplift.

& I fixed base of the dam by fixing it in U1, U2 direction.

When I apply uplift pressure with the fixed base case - the model completes the analysis, but there is no difference between the results of Hydrostatic and Uplift pressure step.

And if I release the U2, than the model does not complete the analysis and shows error "Excessive distortion at a total of 5 integration points in solid (continuum) elements".

Please guide me, How to resolve this problem and to apply Uplift pressure... It would be really helpful to me and appreciated a lot by me.

My citations used to appear on the profit;e page now it is zero

I have a question about some weird nuclei I see while imaging. I'm sorry if this isn't the best place to write it!

One image has bad, "shattered"-looking nuclei from dentate gyrus.

The other image has healthy nuclei from CA1.

I end up in some experiments with really terrible looking DAPI with "shattered" nuclei. Sections with this type of DAPI are highly correlated with really bad FISH staining as well. It seems like most of the tissue integrity is lost in general.

I've seen this before, but not enough times to know what step of tissue collection and processing could cause it. I don't *think* this is purely a cryosectioning issue.

Does anyone have any guesses for what could cause this issue? I'm guessing I can't be the first person to run into this!

I am imaging coronal brain sections from mouse tissue that is flash frozen immediately after dissection. In this case this is imaging dentate gyrus, where nuclei should be exceptionally dense. 20 um cryosections are immediately placed onto Superfrost glass slides and stored at -70 (in this case for ~2 weeks).

Sections are fixed with 4% PFA on the slide and then dehydrated with serial EtOH incubations of 50%, 70%, and 100% for 5 minutes each. Then sections go through the RNAscope smFISH protocol, at the end of which they are stained with DAPI for 30 seconds.

Today I found a very strange thing in my research gate account..it showing Citations -0..However, I saw more than 150 Citations last week..Dose anyone know how to fix it ??

I am working on termites and doing in situ hybridization but at the end, I can't see cells in specimen slides. my question is how to maintain the structural integrity during fixation or whole process can influence cell structure. I think fixation is the main part of hybridization. I am using 4% paraformaldehyde at 4degree to fix termite for 3-4 hours then gradient dehydration with ethanol. if any suggestions it would be highly appreciated.

A growth mindset refers to the belief that individuals can develop their abilities through dedication and hard work. Those with a growth mindset perceive challenges as opportunities for growth and regard failure as a natural aspect of the learning journey.

I'm not sure how to interpret or fix the sample so that it creates a normal band. I used Percoll Gradient with density gradients to generate the questionable band. It's supposed to represent basolateral membrane fraction, and most people mentioned that the sample preparation is not good enough, but I'm not sure how I can make it "good enough".

Log(output) file preview is as below:

Search did not lower the energy significantly.

No lower point found -- run aborted.

Error termination via Lnk1e in /opt/g16/l508.exe

Job cpu time: 0 days 14 hours 4 minutes 28.9 seconds.

Elapsed time: 0 days 2 hours 54 minutes 57.4 seconds.

File lengths (MBytes): RWF= 138 Int= 0 D2E= 0 Chk= 8 Scr= 1

Hello,

I am using movestay command for ESR model analysis, but I get an error message.

Can anyone help me please?

movestay (ln_wage = $x), select(union= $x msp) vce (robust)

The error message is:

Fitting initial values .....initial vector: copy option requires either a matrix or a list of numbers

r(198);

Thanks in advance for your kindness

Why am I getting a Parsing error when I upload an article? How do I fix it?

I already did some activation experiment to observe expression of CD40, CD86, CD80 and MHC with macrophage 264.7 and dendritic cells (DC2.4). I treat these cells with positive control LPS and therapeutic nanoparticles or bare nanoparticles. So, once I collected the cells, washed them and fixed with 4% paraformaldehyde (pH=6.9) or 5% Formalin in PBS. But I observed that I was losing cells with washing, fixing and post-fixing washing steps. My events/second in the flow cytometry were very low. I was thinking what if I don't fix the cells and I was wondering what was the purpose of fixing?? If I don't fix the cells, should that affect the results negatively? Thanks

Why are numbers and shapes so exact? ‘One’, ‘two’, ‘point’, ‘line’, etc. are all exact. But irrational numbers are not so. The operations on these notions are also intended to be exact. If notions like ‘one’, ‘two’, ‘point’, ‘line’, etc. are defined to be so exact, then it is not by virtue of the exactness of these substantive notions, but instead, due to their being defined so, that they are exact, and mathematics is exact.

But on the other side, due to their being adjectival: ‘being a unity’, ‘being two unities’, ‘being a non-extended shape’, etc., their application-objects are all processes that can obtain these adjectives only in groups. These are pure adjectives, not properties which are composed of many adjectives.

A quality cannot be exact, but may be defined to be exact. It is in terms of the exactness attributed to these notions by definition that the adjectives ‘one’, ‘two’, ‘point’, ‘line’, etc. are exact. This is why the impossibility of fixing these (and other) substantive notions as exact misses our attention.

If in fact these quantitative qualities are inexact due to their pertaining to groups of processual things, then there is justification for the inexactness of irrational numbers, transcendental numbers, etc. too. If numbers and shapes are in fact inexact, then not only irrational and other inexact numbers but all mathematical structures should remain inexact except for their having been defined as exact.

Thus, mathematical structures, in all their detail, are a species of qualities, namely, quantitative qualities. Mathematics is exact only because its fundamental bricks are defined to be so. Hence, mathematics is an as-if exact science, as-if real science. Caution is advised while using it in the sciences as if mathematics were absolutely applicable, as if it were exact.

Bibliography

(1) Gravitational Coalescence Paradox and Cosmogenetic Causality in Quantum Astrophysical Cosmology, 647 pp., Berlin, 2018.

(2) Physics without Metaphysics? Categories of Second Generation Scientific Ontology, 386 pp., Frankfurt, 2015.

(3) Causal Ubiquity in Quantum Physics: A Superluminal and Local-Causal Physical Ontology, 361 pp., Frankfurt, 2014.

(4) Essential Cosmology and Philosophy for All: Gravitational Coalescence Cosmology, 92 pp., KDP Amazon, 2022, 2nd Edition.

(5) Essenzielle Kosmologie und Philosophie für alle: Gravitational-Koaleszenz-Kosmologie, 104 pp., KDP Amazon, 2022, 1st Edition.

We are performing an experiment to detect radon with the help of Emanatory technique and we are not able to fixed the right EHT voltage for Lucas cell PMT. Can anyone suggest how to proceed?

Dear colleagues,

I am encountering an issue with our recently donated Sciex API 4000 LC-MS/MS system. As indicated in the attached data, I am not receiving any signal above 175 m/z. Has anyone else faced a similar issue, or does anyone have insights into the possible reasons behind this? Your suggestions and expertise would be highly appreciated. Given budget constraints, I am exploring whether I can troubleshoot and resolve this without calling in an engineer.

Thank you for your assistance.

Up to half of tropical forestland cleared for agriculture isn’t put to use, research shows!

I did IHC using fluorescent labeled secondary antibodies to detect astrocytes with GFAP marker. My sample was fixed frozen mouse brain tissue streatum region. I saw fluorescence in my sample treated without the primary antibody. I noticed that this fluorescence was exactly overlapped the DAPI staining. I used alexafluor488 secondary antibody and mounting media with DAPI. I attached the picture with my staining. My secondary antibodies were at a 1:1000 dilution.

I am doing CLSM (confocal laser scanning microscopy) of nafion film. I am doing z-scanning. But i am having problem in setting the Z-interval.

For some reason Researchgate combined my citation list with that of Michael D. Baer

which could mislead those who are interested in one of these lists.

I am trying to cut mouse brain fixed in 10% formalin for 24 hours embedded in OCT after sucrose treatment. The samples sink in the sucrose, so we don't think that is the problem and the tissue looks completely fixed based on color, firmness, etc. The issue is that our samples splinter when cut. I have heard this is because the sample is too cold, but we have tried at a range of temperatures and the problem persists. I have also tried adjusting blade angle and changing the blade, but this did not seem to help.

Does anyone have any suggestions or a good protocol to follow?

Thanks,

I have a problem with running my logistic regression. When I run my analysis, I get really strange values and I cannot find anywhere how I can fix it. I already changed my reference category and that led to less strange values but they are still there. Also, this only happens to two of my eight predictors. These two predictors have multiple levels/categories.

Can someone explain to me what's wrong and how I can fix it?

The URL assigned for my researchgate landing page is incorrect - it has my name wrong. How do I correct it to reflect my correct name?

I am running protein samples on SDS-PAGE that are tagged with fluorophores. If I place the gel in a destain solution of 60%water/30%methanol/10% acetic acid, the chemistry reverses and I lose the fluorophore. Is there an alternative storage solution I can use to get my gel to the imager to capture the fluorescence without chemical fixatives? After imaging, I then proceed to Coomassie Blue staining in acetic acid and methanol, at which point I am not worried about losing the fluorophore.

Hello experts from Research Gate,

I am struggling with staining live E.coli with Hoechst 33342 and FM 4-64FX. The fluorescent images were too dim even at high concentration of the fluorescent dyes (2-10 times higher than the concentration recommended by Invitrogen). And I had to acquire images at high exposure time (1000-4000ms) which led to photobleaching, high phototoxicity, high noise and extremely slow image acquisition. It seemed that Hoechst could not penetrate all the live E.coli cells uniformly (a few were bright and many were too dim).

Could you please have a look at my protocols and point out the problem of the protocols.

Protocol 1. Staining with Hoechst 33342 (or SYTOX Green):

Protocol 2. Staining with FM 4-64 FX after staining with Hoechst (or SYTOX Green):

Protocol 3. Staining with FM 4-64 FX alone:

################

Note:

Hi every one

I have a model in Abaqus standard with Heat transfer. When I restart this model , Abaqus exit with this error:

"Unable to open the file <rank=0,arg_name=E:\ABQ

Model>Job-2.mdl"

How I can fix this error?

Thanks

The way in which the contractual dispute was formed was that the fixed obligations of one party made it an economic force of the contract that could not be resisted, while the immutability of these obligations made the other party something rigid. This constitutes “a fundamental loophole in the contract, to prevent the fragmentation of the contract’s economy.”

I am writing a word document, using my Mac. When trying to insert a citation from Mendeley I get a Microsoft message saying "Citation/Bibliography is wrongly placed in index area, please delete the placed citation/bibliography in index area."

What should I do?

Many thanks

What does DAMASK runtime interrupt with the error "max number of cut back exceeded, terminating" mean, and how do I fix this problem?

Even fixing memory limit and processor unit does not work.

Hi all,

I am a PhD candidate trying to model a fluidized bed reactor with liquid-solid fluidized bed reactor. There is no gas. The solid fraction is not fixed at the moment so for maximum flexibility of solid fraction and for ease of using the code (easy to understand and modify). Which opensource LBM software would you all suggest? Thanks in advance!!

Hlo I am trying to get kinetics of methanol to dimethyl ether kinetics on hzsm-5 si/al = 40 catalysts at 160 deg c I was unable to reach zeroth order kinetic regime. I doubt that the reaction will become mass transfer control before it reaches zero order kinetics. Please let me know what should be the partial pressure of methanol when the reaction reaches zero order . I am also using a fixed bed catalytic reactors where i change the flow rate of methanol to change the partial pressure and i am also injecting nitrogen along with the methanol where i am changing the methanol flow rate. Please let me know is this an ideal practice for getting kinetics. Please let me know

Thank you.

Vignesh

After performing PCR, I ran electrophoresis, but on agarose the results showed some rather blurred samples. I wanted to know the cause and how to fix this situation. Please note that the chemicals and dyes are normal because the positive control shows a clear band.

Hello i tried to calculate the size of cristallites with ORIGIN drawing a line passing by the middle of each peak . the problem is that there are some peaks where the line does not pass by them how can i fix this .If there is another methods to calculate the size of cristallites ?

I thawed these adherent cells 3 weeks ago, I don't remember when I found this contamination,

Contaminants and cells do not grow in the same layer and contaminants appear to be irregular or filamentous，

the cells are growing well, and the medium is always clear,

the blurred dots and lines are because the microscope is not clean, that is not contamination.

but I wish to fix this problem before it becomes worse,

If anyone has any suggestions, that would be a great help to me, thanks a lot.

I have to remove OCT from the tissue completely and fix the OCT-removed tissue in paraffin. Please suggest the best way.

I need to do fluorescent microscopy using Propidium Iodide. I initially fixed my cells with 4% paraformaldehyde and saw red stain in Control cells. It turns out PFA is cell permeable. So if anyone has a protocol using Ethanol as a fixative please do share.

A large polymeric membrane was recovered with graphene oxide. I would like to know where the graphene oxide fixed and how is the distribution.

Has anyone purchased or used the table top BD Facs melody cell sorter? Our lab purchased one 1.5 years ago and it has been the most frustrating machine ever. The stage locks up, techs have to come out every 2-3 months to fix something and reboot the software, etc. I am trying to ascertain other researchers experiences , if any, with this model. I believe we have an absolute lemon but I want your feedback.

Neutral buffered formalin is best fixative in histopathology. But if by any chance it gets yellowish so what all shall we do to get rid of this problem in laboratories?

An example can help better to understand and solve this.

I am a co-author of the article:

DOI: 10.1051/0004-6361/202142242

This article is presented on the RG by the research item:

in which not all authors are listed (only 21 among 36).

Because of this mistake in the metadata, 15 researchers (including me) cannot verify their authorship. How can I add missing authors to this research item?

I just doing synthesis for Quartenary Ammonium Chloride in my Lab. When I analysis using FTIR ATR, there's one peak with 150% transmittance. How to fix it? If there's any possibilities for a peak above 100%?

Hi,

quite specific question if somebody has experience with this column because I cannot pull out fritta and pack column with stationary phase.

Dear All

I need help in troubleshooting mossy fiber recordings. All my PhD and Post-doc experience so far was in SC-CA1 recordings (Both field and whole-cell recordings). For a new project, I am trying to record mossy fiber-evoked responses in CA3 cells with a low current stimulus strength (20-60uA). However, I am seeing a lot of polysynaptic activity in the recordings. I initially started the stimulation with a cluster bipolar electrode (which I frequently use for SC-CA1 recordings). However, most of the mossy fiber recording papers used a glass electrode for stimulation. So, I realized I might be stimulating a large set of fibers and hence changed the stimulating electrode to a monopolar glass electrode filled with recording aCSF (3-5MOhms, same pipettes used for CA3 recordings as well). But I still see a lot of polysynaptic activity and spontaneous activity.

Cutting and incubation in half sucrose and half NaCl-based solution. The hippocampal slices were cut at 10 degrees magic-cut whole hemispheres, both based on protocols from Peter Jonas's lab.

Recordings were obtained in a regular aCSF with 100uM PTX (No NMDAR blocker as I am clamping at -70mV). I do not have a fixed place for stimulating electrode position, I keep varying it from the inner DG to Hilus to proximity to the recording electrode. All positions gave me similar results.

I do start evoking within 3 minutes of breaking in. Should I wait for a longer period like 10 minutes?

I appreciate it if anyone could help me to get rid of the polysynaptic activity in these recordings.

Academia has lots of silly little faults that ought to be simple to fix. For example:

Why are PDF downloads not associated with the author's name and date of production? This is a problem particularly with texts that did not get published in a conventional journal.

What happens when an author dies? There should be provision for updating that person's profile, in case anyone wants to ask that author a question.

How can I expunge duplicate mentions of a publication from my profile's list? Is there a way to put them in chronological order?

Humble users like me need a way to communicate with whoever maintains Academia's Internet system.

Hi everyone,

I established a murine spheroid for glioblastoma, and I want to fix and stain them to conduct immunofluorescences. I have tried several times, but the size of my spheroids are small, and I cannot see them during Paraffin embedding step. I would be grateful if you could give me some hints on doing this step.

My name is misspelled in one of the articles and it is not showing up in my google scholar profile. How to fix this issue? It is very crucial to me for immigration purposes.

I am creating a heat exchanger for the lattice structure core on ntopology to be transferred to Ansys for simulation afterwards. But the meshing is faulty and Ansys cannot process it correctly. Is there any way to fix the intersecting and other types of meshing errors on ntopology? Is there any way to save the ntop filesbefore meshing?

Thanks

Hossein

Explain in detail.

Greetings, the maximum value of diversity gain for MIMO antenna is fixed at 10dB. What is so special about that value? What does DG 10dB mean.

Does anyone have a protocol/experience with extracting total protein or RNA from formalin or PFA fixed tissues that have not been paraffin embedded? I want to extract protein/RNA from mouse brains that were fixed by perfusion with 10% neutral buffered formalin. Older samples are cryoprotected in 30% sucrose, but if I can cut a piece of tissue before the sucrose step that is also doable.

I want to perfuse my mice to better preserve tissues for cryosectioning and IHC, but it would be nice to use less animals and perform less surgeries especially since I only need little protein or RNA to run Western blots or RT-qPCR experiments and it seems a shame spend extra time and mice.

I have searched the literature and see many papers discussing techniques for FFPE sections, but no discussion of fixed tissues that are not paraffin embedded or mounted on slides.

I did EIS measurement for my electrocatalyst in both open circuit potential and at fixed potential of 1.5 V vs RHE. The Rct for OCP came much lower than the fixed potential. What could be the reason?

I calculated the optical properties for NiTiO3 using Quantum Espresso. Even though I did not use USPP, I still got the error: USPP are not implemented. How to fix? Thank. This is my nscf file.

And also, how to solve these warnings that I have encountered?

I modelled a 3d truss with one storey concrete structure attached to it as shown on the model. I know that the one storey concrete structure changes the rigidity and the flow of the forces on the truss, but my third bay truss ( encircled on the figure ) is taking excessive axial forces such that it requires insane angle bar sizes for the chords and webs to pass.

What is the explanation for this? any recommendation on how I can fix this ?

The protocol was designed for RNA sequencing can't be applied because RIN number was low and the cDNA for the same sample was obtained by random hexamer primer. What can i do to fix the situation because i can't do the process of RNA extraction again

Good morning!

Just a quick question out of paranoia: for *fixed* tissue (specifically brains already having been fixed in 4% paraformaldehyde) that are cryosectioned what are the storage conditions (long/short) do you keep them at?

RNA stability is *not* a concern for these samples (Regular protein-IHC will be done, not FISH or any nucleus acid detection that would warrant more care involved in sample prep)

I left some of the cut cryosections in the hood for a few days- do you suppose these samples have severely degraded? Or should it not be a concern? I was always taught that as long as a tissue was fixed it is stable following cryosection but I’m paranoid and would like to make sure.

Thanks for your input! Much appreciated

How do nitrogen fixing bacteria capture nitrogen and type of bacteria capable of converting atmospheric nitrogen into fixed nitrogen which is used by plants?

I am trying to measure Charge/Discharge curves of anode free lithium-metal battery and trying to fix both areal capacity and current density in Landbattery software. For example, areal capacity ~ 0.785 mAh and current density ~ 1 mA/cm2 should be fixed, we can calculate current ~ 1.77 mA by dividing with 0.785 mAh, we can get time in "h" which is 2.25h. Now, should I give a current 1.77 mA as shown in Fig with a time of 2.25 h and then how to fix the voltage? I am really confuse for a measurement setup, I will be very thankful if you can help me in this regard. Thanks

Hi everyone! I tried to perform a classic One Way Anova with the package GAD in R, followed by a SNK test, which I always used, but it didn't work with this dataset, and I got the same error for both tests, which is the following:

"Error in if (colnames(tm.class)[j] == "fixed") tm.final[i, j] = 0 :

missing value where TRUE/FALSE needed"

I understand there is something that gives NA values in my datatset but I do not know how to fix it. There are no NA values in the dataset as itself. Here is the dataset:

temp Filtr_eff

gradi19 11.33

gradi19 15.90

gradi19 10.54

gradi26 11.01

gradi26 -1.33

gradi26 9.80

gradi30 -49.77

gradi30 -42.05

gradi30 -32.03

So, I have three different levels of the factor temp (gradi19, gradi26 and gradi30) and my variable is Filtr_eff. I also already set the factor as fixed.

Please help me, how do I fix the error? I could do the Anova with another package (library car worked for example with this dataset) and I could do tukey instead of SNK, but I want to understand why I got this error since it never happened to me..thanks!

PS: I attached the R and txt files

tissue is human fetal cerebellum in their gestational ages

You have my publication, Place- and Community-based Education listed as an article on Research Gate. However, it's a book. And I don't have a digital copy. I get a couple of requests a month for the article and I can't provide it. Can you please change this so this publication is listed as a book and indicate there's no full copy available from the authors?

Best,

David Sobel

Antioch University New Englan

I am currently trying to run a command prompt for MD simulation but came across this error:

C:\frohaisa\MD>namd2 runme.namd > simul.log

FATAL ERROR: tried before startup to read config file parameter that was not set

while executing

"---------input-----"

(file "runme.namd" line 1)