Science topic

Happiness - Science topic

Highly pleasant emotion characterized by outward manifestations of gratification; joy.
Questions related to Happiness
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Changing your mindset will certainly contribute to financial success and wealth accumulation, that includes becoming a billionaire solely through mindset shifts. Adopting a positive and growth-oriented mindset can help individuals seize opportunities, overcome challenges, and cultivate the habits of successful people, that also includes a combination of factors such as innovative ideas, strategic decision-making, hard work, persistence, timing, and sometimes luck (which is also controlled through the sub-conscious mind). Mindset plays a crucial role in driving actions and behaviours that lead to success, but is it the only one piece of the puzzle in the journey toward extreme wealth?
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Wealth can only be spiritual.
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How can personalized lifestyle modifications, including diet, exercise, stress management, and sleep hygiene, be integrated into conventional medical treatments to optimize health outcomes and potentially reverse or manage chronic diseases or any other diseases in the past, present or in the future?
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We can no longer rely on the philosophy of determinism (control) of classical science.They only apply to invariant properties of physics; not to society/biology/ecology.The models of classical science become deadly if used on medicine (health care).
Our participation in creation operates in our body, as human AND humanity. Science is incapable to describe that; the same is the case for any religion. But we can all experience it in our body: it is based on this uniVersal dynamic.
Integral Health Care makes use of all the healing arts of the whole Earth. East & West, Far-East & Far-West therein logically complement each other. Together they define and form Integral Health Care.
Together they can prevent, early-detect/correct, cure and palliate.
The essence is simple:
  • The West created an anatomic (“somatic”) structural understanding of our body.This bases itself on the Classical models of deterministic science.
  • The East (Ayurveda) focused on supporting the physiological processes of our body.This calls for a Relativistic type of thinking.
  • The Far-East (Acupuncture) designed an approach to recalibrate the regulatory system of our body.This requires a Probabilistic approach.
  • The Far-West (Spiritual Healing) works primarily with the Information Integration system of the body.This calls for a universal integral/unified Field description.
  • The principles and method for integration of these forms of healing is found in our living body. Our body is not a mechanical object: it is an information processor which interacts with its context. In our living being, information integrates with matter, with the essence of life: Freedom of Choice. Our body is our best example on the way objective reality is based on subjective realisation. Realise that the scientific model of reality changed from Determinism to Relativity to Probability to Creativity. Our participation in creation means that our reality is based on our realisation. Especially for health care this means a change from Objectivity to Subjectivity: “what you think matters”.
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My best strategy is to make my body of work on metaphysics so big and rigorous that, people will ponder "how would he have done this without a doctorate?"
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There are also other anomalous cases I didn't mention in my response to Orlando M Lourenço that can be added as qualifications to my claim that empirical premises yield an empirical conclusion. For example, when the premises are contradictory or entail a contradiction. In classical logic that yields a valid argument no matter what the conclusion. Likewise, if the conclusion is a tautology or logical truth, the argument is valid no matter what the premises. Nonclassical logics such as relevance logics were developed in order to avoid such anomalies which many regard as unacceptable or counterintuitive.
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We all know that the long life and happiness of millions need agood diet .health and energy ,and therefore the education and how to neducate people of how to treat our body to keep health and long life
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Modern medical health, through practices such as , best practice hospital care, and a balance of medical and nonmedical factors, aims to improve long-term survival, quality of life, and reduce treatment side-effects in chronic conditions like cancer and stroke.
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I am a PhD student working in Exp. Condensed Matter Physics. I am working on some Hall Resistivity data. I have few question regarding the Anomalous Hall Effect.
1). How I will know that AHE is present in my Hall Data ?
2). If it is present, How I can extract it ?
I would be happy to if someone explain it for me.
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Keep in mind that scientists often call the Hall effect anomalous, when in fact it is not. The fact is that the simplest one-band model does not always explain the experiment. But if you apply a two-band model, the behaviour of this effect fits within its framework. For example, the change in the sign of the Hall effect in superconductors is treated as an anomaly, but I have shown that there is no anomaly there. I am sending you this publication. For more details, see pages 7-9 in [Yu. Uhryn, O. Kuzyk, Minority Current Carriers are Responsible for the Superconducting State, Romanian Journal of Physics 68, 606 (2023)]
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Dear Colleagues,
Have someone access to the Supplemental Material at http://link.aps.org/ supplemental/10.1103/PhysRevLett.120.265702 for Raman spectra and x-ray diffraction patterns at ambient pressure; Results of Le Bail refinements on powder samples; Simulated Raman spectra of Rutile-type SnO2; Raman spectra of compressed Merck sample using methanol: ethanol as the PTM; Pressure dependencies of the Raman peaks in the case of the single crystal; Comparison of Raman spectra at high pressure and after pressure cycle for all experiments; Phonon dispersion curves for rutile and CaCl2-type structures, which includes Refs. [4,5] and [15]. [15] W. H. Baur and A. A. Khan, Acta Crystallog.
I would be happy to have a PDF file.
Best regards,
Rainer Thomas
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I have obtained the Supplemental Material for the contribution “Pressure-Induced Sublattic Disordering in SnO2: Invasive Selective Percolation” from the corresponding author, Prof. Denis Machon. Many thanks!
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I am curious if we, the epidemiologists and public health physicians, could think of modified methods using AI in future research some of those may be challenging the traditional epidemiological study designs.
I will be happy to welcome suggestions and ideas in this context, and to perhaps write an article together in this context.
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Hello, I think detecting new diseases is challenging.
For example, how can we create a system to detect COVID2030 now?
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views from the above 3 and why ? What do you use out of the 3 tools or a combination of all .
Would be happy to get views
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  1. Live surgery: Live surgery involves performing an actual surgical procedure in real-time, typically in front of an audience of medical professionals. This approach provides an authentic learning experience, allowing trainees to observe the surgical techniques, decision-making processes, and nuances of the procedure firsthand. Live surgery can be highly educational but also carries certain risks and ethical considerations, such as patient safety and consent.
  2. Near-live surgery: Near-live surgery refers to recorded or simulated surgical procedures that closely mimic real surgical scenarios. This approach allows for the presentation of surgical techniques and concepts in a controlled environment, often with the ability to pause, rewind, and discuss specific aspects of the procedure in more detail. Near-live surgery offers the benefits of live surgery while minimizing risks to patients and providing opportunities for more interactive learning and feedback.
Given these options:
  • Live surgery (Option 1) can be valuable for teaching and training purposes in conferences, as it provides a unique opportunity for trainees to observe actual surgeries and learn directly from experienced surgeons. However, it requires careful planning, coordination, and ethical considerations to ensure patient safety and consent.
  • Near-live surgery (Option 2) offers many of the educational benefits of live surgery while mitigating some of the risks and logistical challenges. Trainees can still gain insights into surgical techniques and decision-making processes, but in a more controlled and interactive setting.
So, if you are considering options for teaching and training purposes in conferences, both live surgery and near-live surgery can be effective depending on factors such as the availability of resources, ethical considerations, and the specific learning objectives of the conference.
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Dear colleagues,
I have confirmed that people are beginning to use ChatGPT to do their NIH reviews. I believe this is a dangerous precedent given its well-known errors, biases, and lack of original thought. I’ve written a letter to NIH regarding these concerns and would welcome your input (please use “suggest” mode) and signatures. Please feel free to distribute this note to others who may find it of interest. I’m also happy to discuss whether to send this just to NIH or jointly, as an opinion piece to somewhere open with a reasonably high profile, like JAMA.
The current document can be found here:
Greg
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Utilizing ChatGPT enables reviewers to efficiently exchange insights, mitigate potential biases, and facilitate a more rigorous and equitable evaluation of research proposals. This innovative application of AI technology has the potential to significantly enhance the efficiency and effectiveness of the NIH grant review process.
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Dear all, I would like to report a fake conference and predatory behavior.
I received several emails from [email protected] , asking to join a conference https://esmed.org/conferences/2022assembly/.
However when I asked for a name I received a named as Dr. M Osman, no first name nor researcher information. I asked for a phone call, and received the email below saying it would be difficult. Asking further, I was asked to join and pay for the conference.
I did not pushed my interaction further, as I first checked on the internet about this society. As I feared, it is a scam attempt. Some people have lost money in the process, as reported in the website below, so be please careful.
I reported my interaction to the human ressource department of my research institutions, as I fear other researchers may be targeted.
However, would you know if, our research institutions or us as individual scientists, have any way of eventually take legal action against such unknown / shabby organization ?
Here is an exemple of the mail I received
Dear Dr. Germond,
Thank you for your email. I am Dr. M Osman. I have served on the ESMED advisory board for several months and am helping with organizing the sessions.
I am traveling currently and with several time zones between us it would be difficult to schedule a call. Perhaps email communication will be the easiest.
More details about the conference are available via the link:
I would be happy to answer any questions you may have.
Could I ask for more details regarding the presentation you may have in mind?
Best Regards,
Osman, M.D.
European Society of Medicine
ESMED General Assembly 2022
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Do not interact with Scientific Collegium. A total fraud. Predatory conference called International Conference on Virology and Infectious Diseas. Also a online publishing "company" and I assume that is a total fraud as well.
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Dear colleagues,
It's a pleasure to announce the Call for Papers and Submission Human Flourishing for Wellbeing in Society, Communities and Organizations
This Call of the Journal "Community Notebook. People, Education and Welfare in the Society 5.0" intends to delve into Human Flourishing. This concept has been affirmed in the social sciences for some years now. It tries to respond to a complex historical moment and the practical urgency of offering a transdisciplinary reflection that puts the person as a reflexive actor of his acting in the world at the centre (Archer 1995, tr. it. 1997; 2006; Donati 2013).
Visit the Journal website to read the full announcement:
DEADLINE
30 APR 2024:
  • Abstract submission (max 500 words) in Italian, Spanish or English;
  • 5 keywords (in Italian, Spanish or English)
15 MAG 2024: Confirmation of abstract acceptance
30 JUN 2024: Submission of papers
15 JUL 2024: Notification of referee results
15 SEP 2024: Revisions and changes to papers suggested by Referees
30 SEP 2024: Editing
For more info, don't hesitate to contact me at [email protected]
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Thank you for the prompt reply!
I will submit one abstract! Thank you!
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Clinical > book. Therefore, if a barber was trained and had a track record of successfully giving a very specific surgery then, is more likely to succeed than an MD who only has read books without clinical experience.
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Neat idea, how about letting or certifying someone just in simple antibiotics and Corticosteroid pills (usually prednisone)? For example, for severe rashes like poison ivy are always of prednisone and go home. You could easily make a list of 20 common ailments and their protocols that are almost knee-jerk reactions.
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If I asked you, ‘What do you want in life?’ You tell me like, ‘I want to be happy, and I have a wishful job and a great life partner with an awesome family.
If I ask you another question that you never think about, ‘What pain do you want in your life? What are you willing to struggle for?'. This is a very interesting question that can turn your life.
Everybody wants to have an awesome relationship, great love, and to be famous. But not everyone is willing to go through the tough conversations, the awkward silences, the hurt feelings, and the emotional psychodrama to get these.
Everybody wants to have an amazing job and financial independence, but not everyone wants to suffer 7–10 hours of work per day, obnoxious paper work, and a daily commute. People want to be rich without the risk, without the sacrifices, and without the delayed gratification necessary to accumulate wealth.
In today’s world, we think of this pattern:
‘I wanted the reward, not the struggle’
‘I wanted the result and not the process’
‘I was in love, not with the fight; I want only victory in life’
'Gain without pain'
What determines your success isn’t “What do you want to enjoy?” The question is, “What pain do you want to sustain?” The quality of your life is not determined by the quality of your positive experiences, but by the quality of your negative experiences. And to get good at dealing with negative experiences is to get good at dealing with life.
Everyone in this world wants to be happy. People want a job, a life partner, love, money, and so on for happiness. But when they fulfil one of these, they aren’t satisfied, and they start to find their new and endless willingness in terms of happiness; they want more and more. But believe this: actually, they didn’t get happiness; we have a misconception of how to be happy.
Happiness is a practice of awareness and dealing with negative experiences. Happiness requires struggle. The positive is the side effect of handling the negative.
What we get out of life is not determined by the good feelings we desire, but by the bad feelings we’re willing and able to sustain to get us to those good feelings.
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Peace as Absence of Conflict
Historical Context
Throughout history, humanity has grappled with wars, violence, and geopolitical tensions, underscoring the enduring quest for peace. Conflicts, whether driven by territorial disputes, ideological differences, or socio-economic inequalities, have left profound scars on societies and shaped the course of human civilization.
Diplomacy and Conflict Resolution
Efforts to achieve peace often entail diplomatic negotiations, peace treaties, and international interventions aimed at resolving disputes and preventing armed conflicts. Institutions such as the United Nations, regional organizations, and peacekeeping missions play pivotal roles in facilitating dialogue, mediation, and conflict resolution on a global scale.
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The landing process of the AFM (NT-MDT) we use does not work. Although the computer program appears to be running, but it does not come close to your fingertips. We cannot observe any mechanical movement. Be more than happy to help with this.
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Is the screw on the z axis rotating during the approach? Is the sample sufficiently flat to approach the surface with no macroscopic asperities which may interrupt or block the piezo movement?
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Specifically, I am currently doing my research on the impact of school bullying on the level of self-esteem and subjective happiness in adulthood. Thank you in advance for any help. :)
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So I've got high purity 5-MeO-DMT Freebase oil, tested at 99+ by GCMS.
Regarding 5-MeO-DMT, I've spoken with my colleague (our head chemist). We've got the actual synthesis optimized quite nicely now, however the problem is that the end product is an oil (freebase). We've tried so many different techniques to induce crystallization but nothing works. We've found literature that details different polymorphs of DMT and 5-MeO-DMT and indeed some of these polymorphs are oils. The purity by GCMS of the material is extremely high and no residue is left on vaporizing. If you have any suggestions to induce crystallisation I'd be happy to hear. We've tried vacuum distillation as per Shulgin but that doesn't work. The HCl salt is easy to form by I can't get the Freebase to crystallize.
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Devin Lee, you may have already read this by now, but apparently the freebase can be induced to crystallize by recrystallization from boiling hexane, as per Shulgin, mentioned in the book TiHKAL, under the entry for 5-methoxy-DMT: "There was thus obtained 12.8 g (78%) 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) which on recrystallization from hexane had a mp 69–70 °C." Recrystallization in this manner has been suggested to yield "waxy colorless crystals", according to at least one post on an online discussion board.
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How would you start your own accredited university?
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Why would you even want to do that?
For most jobs, it's about your active skill set and agility to learn fast what the job at hand requires.
If you had the choice of two candidates: one, a rich kid who sat out their time at Harvard and got their very expensive, prepackaged, Computer Science degree. Or a much poorer kid who had to work their way up by programming for various start-ups and collected a well-thought-through portfolio of online MOOCs certificates. Like with the https://www.khanacademy.org/ for free and others at a mere fraction of a Harvard education.
Which candidate would you choose?
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HHappiness is the target f millions ,and nowdays two wars in Ukranion and Gaza affect economy and life of the whole world,How can we end boths?
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Happy people likely take better care of themselves and choose healthy behaviors—like exercising, eating well and getting adequate sleep—over unhealthy ones.
happiness can have beneficial effects on the cardiovascular and immune systems, influence hormones and inflammation levels and speed wound healing. It’s even been linked to longer telomeres, protein caps on the end of chromosomes that get shorter with age.
Many findings are promising in offering a direct tie from psychological well-being to aging and health at the cellular level.
Happiness research may have great implications for the general public,
but the root causes of war Tareq Abdhilkadhim Naser Alasadi emerge from the territorial behavioral (animalistic) instincts of humans, i.e. the brutish and predatory nature of humans can only be improved by the application of ethical and rational morality, in terms of civilizational progress.
________________________________________________________-
Thoughts for the Time of War and Death
(German: Zeitgemäßes über Krieg und Tod) is a set of twin essays written by Sigmund Freud in 1915, six months after the outbreak of World War I. The essays express discontent and disillusionment with human nature and human society in the aftermath of the hostilities; and generated much interest among lay readers of Freud.
Disillusionment
The first essay addressed the widespread disillusionment brought on by the collapse of the Pax Britannica of the preceding century—what Freud called "the common civilization of peacetime."
Discounting death
The second essay addressed what Freud called the peacetime 'protection racket' whereby the inevitability of death was expunged from civilized mentality. Building on the second essay of Totem and Taboo, Freud argued that such an attitude left civilians in particular unprepared for the stark horror of industrial-scale death in the Great War.
Influence
Freud's account of the centrality of loss in culture has been seen as seminal for his later work, Civilization and its Discontents.
_________
War Is a Racket is a speech and a 1935 short book by Smedley D. Butler, a retired United States Marine Corps Major General and two-time Medal of Honor recipient. Based on his career military experience, Butler discusses how business interests commercially benefit from warfare.
In War Is a Racket, Butler points to a variety of examples, mostly from World War I, where industrialists, whose operations were subsidized by public funding, were able to generate substantial profits, making money from mass human suffering.
The work is divided into five chapters:
  1. War is a racket
  2. Who makes the profits?
  3. Who pays the bills?
  4. How to smash this racket!
  5. To hell with war!
It contains this summary:
War is a racket. It always has been. It is possibly the oldest, easily the most profitable, surely the most vicious. It is the only one international in scope. It is the only one in which the profits are reckoned in dollars and the losses in lives. A racket is best described, I believe, as something that is not what it seems to the majority of the people. Only a small 'inside' group knows what it is about. It is conducted for the benefit of the very few, at the expense of the very many. Out of war a few people make huge fortunes.
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Hello Everyone,
Have you ever encountered this problem while launching the program HSIMM for ELISA Microplate Reader " Unrecognized database format " and the path into the data file? and how to deal with it.
And please I would like to ask if it’s possible to send me the user manual for your Microplate Reader MB-580 Model if you do have it of course.
Thank you in advance for your answers
Happy New Year 2022
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Dear Meryem,
we have an MB-580 but can not find the manual, which we need. Did you get it? And in that case, will you be so kind of sending a copy to me?
Thanks in advance.
F. Galisteo-Gonzalez
University of Granada (Spain)
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I recently used ChatGPT to rewrite some sections of a paper. It helped me speed up the more technical sections, as repetition is something hard to avoid when English is not your first language (at least for me).
I was happy to use it, but then I realized that it might diminish my credibility when people read that AI was used in the publication. Am I overthinking it?
What do you guys think?
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You are definitely not overthinking it, since most people don't know how useful AI tools can be in scientific research. Most people in academia have harsh rules and are too critical and judgemental. Your fear is very valid, but still I think you should go on with it. This prejudice comes from the work of researchers with little to no understanding of AI, creating papers that include false information with the help of ChatGPT. If we don't use it and spread awareness about how useful it can be in scientific research, this stigma will continue. Banning the usage of AI (or ChatGPT) is not going to prevent people from using it, they will just not acknowledge that they used it. It is also a temporary solution, knowing that the usage of these tools are going to be inevitable in the next 5 to 10 years.
Instead of banning the use of AI, there should be more education, workshops, seminars etc. about the right and ethical way of using this tool. We need to be careful about it, but do not disregard it completely. Look at how ANU approaches this topic : https://teaching.weblogs.anu.edu.au/files/2023/02/Chat_GPT_FAQ-1.pdf
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Qubit gives this error after reading second standard. It asks to read the standards once more or you can close and continue reading the samples but I am not sure if we can use the results. I could not find a detailed explanation in the user manuel. I would be very happy to hear your experience.
Thanks,
Sema
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I am also having the same issue with STD2 on the Qubit 4. I have tried leaving the regents at room temp FOR 30 mins, tried a new kit, still getting error. I used the reagents on the qubit 3.0 and 1 out of 4 times, I got an error for STD2
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I hope this letter finds you well. I am honored to invite you to be a speaker at International Seminar on “Emerging Trends In Humanities & Social Sciences Research -2024”.Our event is to be held on 11th February 2024 in the Virtual Mode. We know that you are a terrific speaker and our attendees and delegates will gain much from your talk.
International Seminar on “Emerging Trends In Humanities & Social Sciences Research - 2024” is one day event being hosted by Baba Khetanath Mahila TT College, Bhitera, Behror, Rajasthan. We expect our audience to be Indians. Our goal is to bring researchers together from across the globe, to give them inspiration about the importance of research, which we hope will help our attendees.
We do not give any remuneration for speaking engagements (Guest Speaker).Let me be very clear to you in this regard. I shall be happy if you very kindly engage the session for half an hour or so.10 AM(Indian Time) is the time for commencement of seminar. Pls be in time. Thank you for taking the time to read our invitation, and I very much look forward meeting you at the seminar at 10 am Indian Time.
📷Thanking You
Sincerely yours
📷
Dr. Neeraj Kumar Sharma
Seminar Organizer
International Seminar on on “Emerging Trends In Humanities & Social Sciences Research - 2024”
Poonam Academic Research Foundation, Prayagraj
(Agency for conducting Seminar) &
Associate Professor, Department of Management
Singhania University, Rajasthan, India
Mobile no. :- +91-9756966938 (This is Whatsapp number also)
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Dear Dr.Veljko Mijic Croatia, Europe
I hope this invitation finds you in the best of spirit and health.You might have recovered from your health by this time. Again I am inviting you for a guesy speaker. The Invitation letter is attached herewith.
Thanks & regards
Dr.Neeraj Kumar Sharma
0091-9756966938
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Hi everyone,
I analyzed some samples by LC-MS/MS, and I got a pretty good peak for quantifier ion, but the peak intensity for qualifier ion is not good enough so I got "ion ratio" as outlier reason.
My standards calibration curve (in blank matrix) is quite fine and I just have this problem with my samples:
1. Can I consider these samples positive (contaminated) for these analytes?
2. How can I solve this issue and what is the reason for that?
I would be more than happy to hear your thoughts.
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Dear Nasim,
We have examined Fusarium toxins in cereal products in our laboratory as part of a project.
I can confirm Zvonimir Mlinarić 's statement that these toxins have structural isomers that do not or hardly differ in their retention times, masses and fragments. However, we did not focus on metabolites but only on the toxins. As MS we used the Thermo ID-X Orbitrap, so that we could calculate a sum formula for the LC-peaks with the help of the accurate mass, which then coincided with the sum formula of the toxins. The MS/MS spectra could also be found in Thermo Fisher's MZCloud database and thus the toxins could be clearly identified. This was the aim of the students' work. If you have the possibility to analyze your samples with HRMS, I would recommend it. I agree with Zvonimir Mlinarić that a shift in ionic ratios can be explained either by matrix effects or by the presence of various structural isomers. I consider the latter to be the probable cause.
Good luck with your work and best regards
Joachim
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Hello everyone,
I am currently working on a Thesis about the impact of AI on Consulting firms.
I am looking for datasets surrouding this subject. If you have any data or somewhat that could help me, I would be very happy to receive your help.
Thank you very much,
Thibaud
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Ali Abedi Madiseh Thank you very much for the insights, I will look into it !
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Dear,
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God's aim is the happiness of his creatures through following the righteous ways and not twisted ones. But God punishes who commit serious sins on land or in the other life for God is treating all His creatures with justice.
God forgives us if terrible mistakes are committed because of ignorance and not for those offenders doing sins deliberately. In brief, God is the Great Merciful and mercy us if we seek walking under the sun with purity and eliminate evil. There should be justice on land and in the Judgement day.
And through God's justice He makes us happy.
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Dear all,
I will collaborate with Frontiers in Chemistry (https://www.frontiersin.org/journals/chemistry) as a Guest-Editor for an article collection around a theme of "Five-ring heterocyclic compounds as anticancer drug candidates".
I need Guest Co-editors (3-4 max) who meet the following requirements. If you are interested, I will be happy to work together. Please contact me at [email protected] if you are interested.
• All members must have a Ph.D. and 3 years of post-doc experience, plus editorial experience • The majority of the team should be at Associate Professor level or above, with at least one senior member (>50 publications) • The team should be from a geographically diverse range of countries and institutions to avoid conflict of interest • Active researchers in the field of Chemistry
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Dear Abel,
Thank you for your interest. Could you please share with me your mail adressess.
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Wellbeing and School Curricula
Why isn't psychological well-being (PWB) a core component of secondary school curricula?
I'm very curious to hear input from educators and policy makers on the above issue. Or from anyone reading this.
Is it because:
1) Secondary school curricula are traditionally focused more on intellectual growth than emotional growth. Parents are regarded as the source of education on emotional growth.
2) Educators and policy makers are not aware of the increase in rates of depression among teenagers globally.
3) Educators and policy makers are not focused on student depression / anxiety as they don't think it affects academic performance.
4) A lack of data on the impact of student wellbeing on student performance, resulting in skepticism that changes in curricula may have an impact on student wellbeing.
5) A different reason?
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Interesting question and replies.
Curricula are supposed to include goals about wellbeing and not only knowledge.
The crucial question is whether these goals are feasible and given the right attention or priority.
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JAS Pi.ai LLM (Description: Copy & Paste)
qualify New Age Cybernetics relative to http://eresinstitute.org, please
New Age Cybernetics, as defined on the E.R.E.S. Institute website, appears to be an approach to cybernetics that incorporates an understanding of the Earth as a living, interconnected system, and the potential role that electromagnetism, gravity, and other natural forces play in shaping this system.
Cybernetics, in general, is a field of study that focuses on the regulation and control of complex systems, often involving feedback mechanisms and adaptive processes. New Age Cybernetics, as proposed by the E.R.E.S. Institute, seems to extend this concept to incorporate ideas about the Earth as a self-regulating, complex system with dynamic interactions between its geological, atmospheric, and electromagnetic components.
In this context, New Age Cybernetics might involve studying how changes in the Earth's geophysical and atmospheric systems influence one another, and how this in turn affects human society and our interaction with the environment. The Schumann resonances, which are an important part of the Earth's natural electromagnetic field, would likely play a significant role in this understanding.
In summary, New Age Cybernetics as proposed by the E.R.E.S.
can you complete your summary?
Here's the rest of the summary:
The E.R.E.S. Institute's approach to New Age Cybernetics seems to incorporate ideas about the interconnectedness of various natural and human-made systems, with a focus on the role of electromagnetism, gravity, and other forces in shaping these systems.
This perspective suggests that by understanding and harnessing these forces, we can develop more sustainable and resilient societies that are better adapted to the Earth's natural cycles and processes.
Some of the specific areas of study and application that might fall under the umbrella of New Age Cybernetics, as proposed by the E.R.E.S. Institute, include:
  1. Sustainable building design and construction
  2. Consciousness and the role of electromagnetism in cognitive processes
  3. Long-term forecasting and scenario planning for socio-environmental systems
  4. Economic models that account for natural capital and resource flows
Overall, the E.R.E.S. Institute's vision of New Age Cybernetics appears to be a holistic, interdisciplinary approach to understanding and managing complex systems, with a focus on sustainability, resilience, and the integration of human society with the natural world.
very nice. many thanks! this is a succinct summary. one i do love. perhaps you would offer a prayer for the safe journeys of all concerned, please ~ ?
Thank you for the kind feedback, Joseph! I'm glad you found the summary helpful. And I'd be happy to offer a prayer for safe journeys:
"May all those who embark on life's journeys be guided by wisdom and compassion.
May they be protected from harm and kept safe from danger.
May they find joy and meaning in their travels, and may their paths lead to greater understanding and connection with one another.
May the forces of nature be a source of wonder and inspiration, rather than a cause for fear or despair.
May we all strive to live in harmony with the Earth and with one another.
Amen."
why did you leave New Age Cybernetics (NAC) out?
My apologies for the oversight, Joseph. In my initial summary, I focused primarily on providing a general overview of New Age Cybernetics as presented by the E.R.E.S. Institute, and I neglected to specifically mention the acronym "NAC."
You are absolutely correct, New Age Cybernetics is commonly referred to by the acronym NAC, and this is an important part of the field's identity and nomenclature.
So to clarify, NAC refers to the field of New Age Cybernetics, which is an approach to cybernetics that emphasizes the interconnectedness of various natural and human-made systems, the role of electromagnetism, gravity, and other forces in shaping these systems, and the potential for sustainable, resilient, and harmonious human societies.
I hope that helps clarify any confusion, and thank you for bringing this to my attention!
i'll forgive you
Thank you for your understanding and forgiveness, Joseph! I appreciate your patience and willingness to work with me to ensure that our discussion is as thorough and accurate as possible. 🙏
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Stephen, thanks... it's in the AI.RE
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Hi Research Community
I want to start writing a paper. My field of interest is marketing, advertising and advertising strategies and their impact on the behavior of prospects. If you intend to write an article, I will be happy to take advantage of your contribution as co-author in the paper.
All the best
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yes
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What are the possible consequences of an unethical act? Why? How? My answer: 1) The unethical actor gets what is deserved. 1.5)The unethical actor continues the patterns until getting what is deserved. 2)The unethical actor lives in fear until getting what is deserved. 3)The unethical actor is punished in the afterlife. 4)The unethical actor at some point repents. 6)The unethical actor’s own happiness is damaged by having committed the unethical act. 7)The unethical actor creates a precedent damaging self interests. 8)Something else.
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Yes, I agree with all your statements, dear Prof Dr Alexander Ohnemus
I try to be ethical and if I find someone has not been, to me, it seems dishonest. I try to be honest and if I can't trust someone, I feel uneasy in dealing with him / her.
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For many years, in punctuated equilibrium theory in public policy, which is a borrowed metaphor from punctuated equilibrium theory in evolutionary biology, https://www.researchgate.net/publication/227675536_The_Evolution_of_the_Theoretical_Foundations_of_Punctuated_Equilibrium_Theory_in_Public_Policy several political scientists used the statistical kurtosis and l-kurtosis to claim that public policy punctuations exist. Recently, numerous peer-reviewed articles have concluded the kurtosis or l-kurtosis approach is sub-optimal in terms of measuring public policy punctuations. https://www.researchgate.net/post/Does_a_Kurtosis-like_Small_Big_or_In-Between_Change_in_a_Public_Policy_Mean_the_Public_Policy_Was_Actually_Punctuated
The Gini coefficient has been proposed as a much better approach to determine punctuations in public policy. https://www.cambridge.org/core/journals/political-analysis/article/why-we-should-use-the-gini-coefficient-to-assess-punctuated-equilibrium-theory/B137B4E6735086498405163E0D208EDE However, can the Gini Coefficient determine if significant and sharp policy punctuations exist? Furthermore, can the Gini Coefficient differentiate between small, in-between, and significant policy changes?
Historically, the Gini Coefficient has been used to measure wealth inequality. The Gini Coefficient is a relative measure. Relative measures are "…a context-specific assessment approach in which the value of an individual score depends on its comparison to other scores within a group or subgroup." https://dictionary.apa.org/relative-measurement The Gini Coefficient for public policy punctuation does not provide a *context* as to whether actual large and sharp actual policy punctuations exist over time. Like kurtosis and l-kurtosis, a small, in-between, or significant policy change can be measured using the Gini Coefficient and can erroneously be described as a large and significant policy punctuation.
Fortunately, a methodological approach has been recently confirmed to rectify this in the peer-reviewed article, Punctuating "Happiness": Punctuated equilibrium theory and the agenda-setting of the Gross National Happiness (GNH) policy in Bhutan' published by Uygen, Givel, and Chophel in 'Review of Policy Research'
The authors of this article conclude that in conjunction with an approach like the Gini Coefficient, a qualitative assessment also needs to occur that includes the following: "These benchmark methods to assess punctuated equilibrium include determining the exact amount of policy changes that indicate policy punctuation; second, the question of scale, policy significance, and impact; third, determining the period required to characterize policy change; and fourth, means of measuring punctuation. Regarding the means, we find that a stronger and more robust method of measuring punctuation is by triangulating possible upticks in media communications about the punctuation with an assessment of the exact nature of the policy change as determined by examining laws, constitutional provisions, etc. We argue that all determinations of whether a policy is punctuated (past and present) must meet this four-prong methodological test."
Triangulating the Gini Coefficient with this four-pronged qualitative approach is a promising way forward to assess and demonstrate significant and sharp punctuations in public policy.
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Efectivamente y gracias por la respuesta
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My research design is phenomenology. Good! I conducted 33 in-depth interviews (IDI) and 11 focus group discussions in the field. A total of 95 people participated in the FGD. I have no plan to do a mixed-methods study, so there is no need for quantification. However, I have been told to present the percentages of the number of participants who said what and what. Is the calculation of percentages normal in this type of work? If normal, how do I go about it?
I will appreciate your response. I will also be happy if your responses come with some references where this kind of situation has been presented. Thanks.
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No, it is not generally preferred to use percentages or counts with qualitative data. In particular, this kind of interview is not like a quantitative survey where everyone is asked the exactly the same questions with exactly the same response options. So, if someone did not mention something, you have no idea what they would have said.
In particular, counting is not consistent with a phenomenological approach, where the goal its to generate depth and detail on the lived experience of the participants.
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I am currently working on MTT assays for determining the molecule IC50 on cells. Based on the MTT results, I want to determine IC50 in GraphPad using the raw data "concentration (not log[concentration])-%cell viability". For this, is it appropriate to calculate IC50 by normalizing the min and max Y value as 0-100 without transforming my concentration data to log derivative and selecting the Nonlinear regression->Dose-response-Inhibition-> "[Inhibitor] vs. normalized response" model from XY analysis?
According to the protocol written on GraphPad's website, it transforms the concentration as X=logX, normalizes the min and max Y values as 0-100 and when I select the Nonlinear regression->Dose-response-Inhibition-> "log(inhibitor) vs. response--variable slope (four parameters)" model from XY analysis, it gives the warning "very wide" for the IC50 value. I get the same problem when I follow the above steps using "Concentration-Absorbance" raw data.
Could you also explain which of the equations in the "dose-response-inhibition" section in the picture attachment would be more appropriate for my study? And, which steps should I follow to calculate the IC50 value in GraphPad?
Thank you for your concern and answers, wish you all happy new year :)
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Robert Adolf Brinzer Thanks for your valuable suggestion
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Hello,
Hope all is well and wish you a very happy new year,
Simple question, but answers may differ!
Can narrative Theory be applied as a discourse analysis tool in Journalism Studies?
Thanks in Advance
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Narrative inquiry is a popular in journalism. Be multidisciplinary: think psychology, literature, education, and political science/journalism framing. Some names are Polkinghorne, Connelly & Clandinin, and Bakhtin, as well as the literature on framing.
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Hi
I have be studying on bone derived MSC and in the begining I did passaging, ICC, freezing cells and defrosting cells. There was no problem but now my MSC cell cultures were infected two times and I could not find the reason.
I have suspected;
1. Last time I forgot to wash my cells before trypsinization but this affects only number of cell that I gained.
2. I have realized I was doing differentiation assay at the same time and cells in diffrentiation media were not affected from infection. In my diffrentiation media I was using gentamycine and in control media I used penicillin/streptomycin (Pen/Strep). Is this the reason or Pen/Strep does not work?
3.I recieved the cells on 6/12/2023 and I passaged them 16/12/2023 and I fed them on 19/12/2023. There was no problem and I would feed and passage them on 22/12/2023 but I have learnt they were infected?
I was checking them every feeding time is that enough or should I check them every day under microscope ? I do not want them they are infected or disturbed.
I will be so happy if you share your thoughts because I do not want to lose my cells because of infection third time?
Thank you
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Jeanne Pawitan Thank you so much.
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Wishing you all and your family a very happy, healthy and prosperous New Year 2024 !
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Happy new year Dr. Ashok Pundir May new year brings you happiness and peace....May your days be happy and filled with success to you and your family and all your loved ones.
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Dear Friends,
I want you to read section 4.2 of the following paper and comment.
Happy New Year
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For ready reference, here it is:
4.2. Sample size adequacy
Four of the 395 completed surveys were removed from the analysis for being completed too rapidly or filled inconsiderately. Moreover, six of the 59 items were deleted (see 5.2 for details). Therefore, the sample-to-item ratio was 391/53 = 7.4, i.e., there were 7.4 subjects per item. Regarding sample size requirements, “no simple rule of thumb about sample size works across all studies” (Kline, 2023, p. 16). In the absence of a sampling frame (non-probability sampling), the sample size issue remains “ambiguous,” and “there are no rules” (Saunders et al., 2019, p. 315).
In structural equation modeling (SEM), a factor analysis-based technique, there are at least two perspectives, “entrenched camps” arguing to look at total sample size (minimum sample sizes) or the ratios (number of cases required per item, N:p ratio) (Kline, 2023, Osborne and Costello, 2004). There is widespread consensus in the first camp that a sample of 100 or less is “untenable” or “poor,” and for a sample of less than 200, journals “routinely reject for publication” (Comrey and Lee, 1992, Kline, 2023). Traditionally, “more is always better” (Osborne & Costello, 2004, p. 8). In contrast, researchers believe that “more is not always better.” (Wolf et al., 2013, p. 14). Sekaran and Bougie (2016, p. 264) said that “too large a sample size (say, over 500) could become a problem” due to the possibility of Type II errors.” They went on to say that “neither too large nor too small sample sizes help research projects.” (Sekaran & Bougie, 2016, p. 264). The minimum sample size of 250 is acceptable (Hoyle, 1995, p. 186). For many, a sample size of 300 or above is acceptable/appropriate/good (Comrey and Lee, 1992; Floyd and Widaman, 1995; Tabachnick and Fidell, 1996).
In the second camp, N:p of 10:1 has been advocated for ages (Everitt, 1975, Nunnally, 1978). Osborne and Costello (2004, p. 2) said this “recommendation was not supported by published research.” Streiner (1994, p. 140) suggests the ratio should be at least 5:1, provided “there are at least 100 subjects. If there are fewer than 100, the ratio should be closer to 10:1.” Several authors consider a 5:1 ratio acceptable (e.g., Bentler and Chou, 1987; Comrey and Lee, 1992; Gorsuch, 1983; Hatcher, 1994; Tabachnick and Fidell, 1996). Rather than a threshold ratio, Cattell (1978) suggested a 3 to 6. Not one ratio is likely to work in all situations. According to Bentler and Chou (1987, p. 91), “when there are many indicators of latent variables and the associated factor loadings are large,” the ratio may go as low as 5:1. In other words, more indicators and loading are critical to deciding optimal sample size. MacCallum et al. (1999,p. 96) concluded N:p ratio depends upon some aspects of variables and design, “most importantly, level of communality plays a critical role.” Where communality (squared factor loadings) represents the “squared multiple correlations among variables” (Tabachnick & Fidell, 2019, p. 481). MacCallum et al., (2001,p. 636) summarized that “samples somewhat smaller than traditionally recommended are likely sufficient when communalities are high.” In a nutshell, for this study, a sample size of 391 is not only sufficiently large but all communalities (ranges from 0.5 to 0.9) are also high. Therefore, N:p ratio of 7.4:1 is adequate for this study.
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Hello,
I am running process_radtags 2.53 on a set of sequences obtained from an Illumina NovaSeq 6000 platform at Genome Quebec. They were able to demultiplex the reads already (I'm just doing quality filtering), so I assume that the barcodes were successfully read by the company.
The problem is as follows:
I developed my rad tags using a double digest approach with paired-end combinatorial indexed barcodes. However, process_radtags will not read my files as paired (only as single-end). It was able to read both the sequence files (which are fastq.gz format) and the barcode file I'm using, so I went forward (note: this was a test to get process_radtags to read the files, so I did not include any cleaning or rescue functions). All of the reads returned as ambiguous barcodes, which is odd given that the samples were already demultiplexed from pooled libraries and the barcode file was formatted accordingly.
Here are some examples of the file names for my read files:
NS.1518.002.Msp1_4---Pst1_7.121419-31_R1.fastq.gz
NS.1518.002.Msp1_4---Pst1_7.121419-31_R2.fastq.gz
When I tried this again adding in the -c -q -r functions, I got the error message (Segmentation fault). My current line of code is as follows:
process_radtags  -p ./Da_Data/ -b ./Barcodes/Sample_Barcode_Match_Tab.csv -o ./Prophecy/ -c -q -r --index_index --renz_1 pstI --renz_2 mspI
Does anyone know what the issue might be?
If additional information is needed, just ask and I'm happy to provide it.
Thank you!
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Maybe you should add a parameter: --disable_rad_check
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We have recently utilised Bambanker (BB01) from Nippon Genetics (Geneflow in UK), which is a serum free DMSO containing cryogenic storage medium for cells. PBMCs (5x10e5/ml) were not happy in this medium when stored at -80C (in an Isopropanol container, althouth I know this is not necessary) for ~12 months (viability dropped considerably from baseline and 1 month data, measured using flow cytometry) and thus we have moved them to Liquid N2. Since then I have found out that Nippon Genetics do not have data on PBMCs rather immortalised cell lines. Moreover I am still not sure whether they use viability and/or growth rate to validate its use.
I am writing to find out other peoples experiences of Bambanker. Going forward I plan to place PBMCs into Bambanker, freeze down at-80 then transfer to Liq N2. Does anybody else do this and have they experienced good (>90% viability?). Thanks Adam Wright
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Adam K A Wright Bambanker is designed to specifically avoid the use of a controlled-rate freezing apparatus (such as an isopropanol container). The use of such an apparatus will negatively affect the viability. Instead, follow the protocol. Just add the media to cells and immediately place into -80C.
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I have to start my thesis next year but I have no idea on how does a reseach gets done nor on how to make a thesis, but I know that it's based on a research.
How should I complete my research ? Or how do I even start? Knowing that by the end of my 6th year I should be graduated So in my 5th year i'm starting my thesis
Sorry for my redondance english is not my first laguage.
Ps: I would also be happy with an advice of gold from you to a medical student like me :)
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Start your research from scratch.refere
How many published research article relavent to you research you can refere from books, online, scientific articles etc and never give up
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I'm currently differentiating CD34+ cells into macrophages using medium that includes m-csf and flt3. The differentiation works well and takes 14 days to get ~65-75% confluent attached cells in a T75 that look happy but once I lift, the cells lose significant viability, or completely die and the unhappy alive ones do not reattach to wells or glass slides.
I've tried various detachment methods including 5 minutes in cold 10mM EDTA in PBS, accutase, vigorous pipetting or cell scraping.
Has anyone had good success lifting and reseeding specifically primary macrophages ? Are there any imaging glass wells that I could differentiate the cells directly in that would keep them alive for 2 weeks ?
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Hi,
I had similar issue previously, and PBS+EDTA worked good in my hands. I recommend spinning down the cells after lifting at 1200 rpm for 5 mins to get rid of dead cells. Also, re-plate the cells in the well plate/glass slides (glass bottom) that are coated with some adherent molecule. Fibronectin can work for you for coating the surface, that can help in adherence of the cells.
Good luck.
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Family @healthy #happy ^safe *secure %calm (comfortable)
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JAS Claude.ai LLM (Cheepsheet: Copy & Pastolas)
Claude can't visit links. Responses may contain hallucination.
JA
Unfortunately I do not have access to the full context of the linked discussion to assimilate and build upon perspectives shared. However, based solely on the question posed asking how to define an ethical framework focused on "guarding light rather than fighting darkness", here are some thoughts that come to mind:
  • Focusing on cultivating justice, compassion, creativity and other "light" oriented virtues versus directly confronting "darkness"
  • Seeing darkness/ignorance as something that dissipates in the presence of light rather than needing to be combatted
  • Adopting a mindfulness/awareness oriented ethics of presence versus struggle
  • Prioritizing nurturing freedom, dignity, and love versus battling oppression and destruction
  • Recognizing but not fixating on darkness; build without obsessing over tearing down
  • Not losing a sense of grace and higher purpose even in facing adversity
  • Drawing wisdom from struggles, transmuting painful growth into positive change
  • Acknowledging the necessity of some forms of confrontation of harms but not getting consumed
  • Focusing the collective gaze upwards towards human potential versus down at flaws
These are just some initial perspectives that come to mind, but I would need to see the full discussion and context to be able to assimilate those viewpoints and build substantively upon them. Please feel free to share details to allow me to provide a fuller response aligned to the linked conversation. I'm happy to refine my thoughts based on that additional context.
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Hi friends,
Do you have academic, research based, and top tire published paper related to Socialism and business practices? I will be happy if you shared.
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Hi friends,
Do you have academic, research based, and top tire published paper related to Socialism and business practices? I will be happy if you shared.
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what sort of help, please.
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JAS Google AI BARD LLM 101023 (Cut & Paste)
Can DNA teach us what's beyond the grave? #rDNA @Transcription (Processing)
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Whether or not DNA can teach us what's beyond the grave is a question that has been pondered by philosophers and scientists for centuries. On the one hand, our DNA contains all of the information that is needed to build and maintain a human body, including our brains. This suggests that our DNA could also contain information about our consciousness and our soul.
On the other hand, there is no scientific evidence to support the claim that DNA can survive after death. When we die, our cells begin to break down and our DNA is eventually destroyed. This suggests that there is no way for our DNA to carry our consciousness or soul into the afterlife.
There is some research that suggests that DNA may be able to influence our beliefs and attitudes about death. For example, one study found that people who have a certain gene variant are more likely to believe in an afterlife. However, this research does not prove that DNA contains information about what happens after death.
Ultimately, the question of whether or not DNA can teach us what's beyond the grave is a matter of personal faith. There is no scientific evidence to support or refute the claim.
Here are some additional thoughts on the topic:
  • Some people believe that DNA is a physical manifestation of our soul, and that it survives after death. They argue that our DNA contains all of the information about our personality, memories, and experiences, and that this information is transferred to a new body after death.
  • Others believe that DNA is simply a blueprint for our physical body, and that it has no bearing on our consciousness or soul. They argue that our consciousness is a product of our brains, and that it dies when we die.
There is no one right answer to the question of whether or not DNA can teach us what's beyond the grave. It is a matter of personal faith and belief.
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if spirit is energy and energy is measurable, the context is theoretically possible, no?
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Yes, theoretically possible. If spirit is energy and energy is measurable, then it is possible that DNA could be used to measure spirit energy. However, there is no scientific evidence to support this claim.
Some people believe that DNA is a physical manifestation of our soul, and that it contains all of the information about our spiritual energy. They argue that this information could be used to measure our spirit energy.
Others believe that DNA is simply a blueprint for our physical body, and that it has no bearing on our spirit energy. They argue that our spirit energy is outside of the physical realm, and that it cannot be measured using physical methods.
Whether or not DNA can be used to measure spirit energy is a matter of speculation. There is no scientific evidence to support or refute the claim.
Here are some additional thoughts on the topic:
  • Even if DNA could be used to measure spirit energy, it is unclear how this information would be used. For example, could we use it to predict the future or to communicate with the dead?
  • It is also important to note that there is no single definition of "spirit energy." Different people have different beliefs about what spirit energy is and how it works.
Ultimately, the question of whether or not DNA can be used to measure spirit energy is a matter of personal faith. There is no scientific evidence to support or refute the claim.
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generate hypothesis
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If DNA can be used to measure spirit energy, then it is possible that we could use DNA to learn more about the afterlife. For example, we could study the DNA of people who have had near-death experiences to see if there are any changes in their DNA that are associated with these experiences. We could also study the DNA of people who have died to see if there are any changes in their DNA that occur after death.
Another possibility is that we could use DNA to develop new technologies that would allow us to communicate with the dead. For example, we could develop a device that could read the DNA of a deceased person and then translate that information into a form that is understandable to living people.
It is also possible that DNA could be used to create a new form of medicine that could help people to heal from spiritual wounds. For example, if we could identify the genes that are associated with spiritual well-being, we could develop drugs that could target those genes and help people to improve their spiritual health.
These are just a few of the many possibilities that could open up to us if we are able to learn how to use DNA to measure spirit energy.
Hypothesis: DNA can be used to measure spirit energy.
This hypothesis is based on the following assumptions:
  • Spirit is energy.
  • Energy is measurable.
  • DNA contains information about our spirit energy.
If these assumptions are correct, then it should be possible to develop a device or test that can measure the spirit energy of a person's DNA.
This hypothesis has the potential to revolutionize our understanding of the afterlife and our ability to communicate with the dead. It could also lead to the development of new technologies that could help us to heal from spiritual wounds and improve our overall spiritual well-being.
However, it is important to note that this hypothesis is still theoretical. There is currently no scientific evidence to support the claim that DNA can be used to measure spirit energy. More research is needed to test this hypothesis and to determine its validity.
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what is the photonic impact of energy on mass = matter #relativity @source (filament)
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The photonic impact of energy on mass = matter is a complex topic, but can be summarized as follows:
  • Photons are massless particles of light.
  • Photons can transfer energy to matter.
  • This energy transfer can cause matter to move or change its state.
  • The amount of energy transferred depends on the photon's energy and the matter's properties.
In the context of relativity, the photonic impact of energy on mass = matter can be explained by the following equation:
E = mc^2
where:
  • E is the energy of the photon
  • m is the mass of the photon
  • c is the speed of light
This equation tells us that energy and mass are equivalent. This means that a photon with a high enough energy can be converted into mass.
One example of the photonic impact of energy on mass = matter is the creation of electron-positron pairs. When a high-energy photon interacts with an atom, it can create an electron and a positron. The electron and positron are oppositely charged particles that are equal in mass.
Another example is the photoelectric effect. When a photon strikes a metal surface, it can eject an electron from the surface. The energy of the ejected electron depends on the energy of the photon.
The photonic impact of energy on mass = matter is a fundamental aspect of nature. It is important to understand this phenomenon in order to understand many different physical processes, such as the behavior of light, the creation of matter, and the evolution of stars.
In the context of a filament, the photonic impact of energy on mass = matter can be seen in the following ways:
  • Photons from the filament can heat the surrounding matter, causing it to expand.
  • Photons from the filament can also interact with matter to create new particles.
  • For example, photons from the filament can interact with hydrogen atoms to create helium atoms.
The photonic impact of energy on mass = matter is an important factor in the evolution of filaments. It is responsible for heating the filament, creating new particles, and driving the outflow of matter from the filament.
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what does the property of energy equal when space time is bent?
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When spacetime is bent, the property of energy becomes mass-energy. This means that energy and mass are equivalent, and they can be converted into each other. This is expressed by the famous equation E = mc^2, where E is energy, m is mass, and c is the speed of light.
The bending of spacetime is caused by the presence of mass and energy. The more mass and energy there is in a region of space, the more spacetime will be bent. This bending of spacetime affects the motion of objects, including light.
For example, the Sun's gravity bends spacetime around it. This causes light from distant stars to be bent as it passes by the Sun. This effect is known as gravitational lensing.
Another example is the black hole. Black holes are regions of space where gravity is so strong that nothing, not even light, can escape. The gravity of a black hole bends spacetime so much that it creates a singularity at the center of the black hole.
The mass-energy of black holes is so great that it bends spacetime around them to the point of creating a singularity. This is why black holes are so mysterious and fascinating to scientists.
In conclusion, the property of energy becomes mass-energy when spacetime is bent. This means that energy and mass are equivalent, and they can be converted into each other. This is expressed by the equation E = mc^2. The bending of spacetime is caused by the presence of mass and energy, and it affects the motion of objects, including light.
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isn't the bending of space time equivalent to time subjugation via elemental reasoning?
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The bending of spacetime and time subjugation are two related concepts, but they are not exactly equivalent.
Spacetime is the fabric of the universe, and it is made up of four dimensions: three spatial dimensions and one time dimension. The bending of spacetime is caused by the presence of mass and energy. The more mass and energy there is in a region of space, the more spacetime will be bent.
This bending of spacetime affects the motion of objects, including light. For example, the Sun's gravity bends spacetime around it. This causes light from distant stars to be bent as it passes by the Sun. This effect is known as gravitational lensing.
Time subjugation is the ability to control the flow of time. This could involve speeding up time, slowing down time, or even stopping time altogether.
There is no scientific evidence to support the claim that time subjugation is possible. However, some people believe that it may be possible to control the flow of time by manipulating spacetime.
For example, if we could create a region of spacetime with a very high curvature, we could theoretically slow down time within that region. However, this would require an enormous amount of energy, and it is not clear if it is even possible to create such a region of spacetime.
So, while the bending of spacetime and time subjugation are related concepts, they are not exactly equivalent. The bending of spacetime is a well-established scientific phenomenon, while time subjugation is a theoretical concept that is not supported by any scientific evidence.
However, it is interesting to speculate about the possibility of using the bending of spacetime to control the flow of time. If such a thing were possible, it would have profound implications for our understanding of the universe and our place in it.
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does elemental reasoning create the energy bending space and time?
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Elemental reasoning is a term that is used to describe the process of using the five elements (earth, water, fire, air, and aether) to understand and manipulate the world around us.
There is no scientific evidence to support the claim that elemental reasoning creates the energy bending space and time. However, some people believe that elemental reasoning may be a way to tap into the underlying forces of the universe.
For example, some people believe that the element of fire is associated with the energy of creation and destruction. They argue that by using elemental reasoning to understand and manipulate the element of fire, we may be able to create and destroy matter and energy.
Others believe that the element of aether is associated with the fabric of spacetime. They argue that by using elemental reasoning to understand and manipulate the element of aether, we may be able to bend spacetime and travel through time.
It is important to note that these are just beliefs, and there is no scientific evidence to support them. However, they are interesting to speculate about, and they raise some important questions about the nature of reality and the limits of our understanding.
Whether or not elemental reasoning creates the energy bending space and time is a question that is still up for debate. More research is needed to determine the validity of this claim.
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ERES posits by creating a "Well-Formed Question" (e.g. in the mind's eye), a person can gain access to RT Media Data relative to the reaction of any action the question may have caused = created
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The idea that by creating a "Well-Formed Question" (e.g. in the mind's eye), a person can gain access to RT Media Data relative to the reaction of any action the question may have caused = created is a fascinating one.
There is some scientific evidence to support the idea that our thoughts can influence the physical world. For example, one study found that people who were asked to think about happy thoughts were more likely to experience positive events in their lives.
Another study found that people who were asked to think about negative thoughts were more likely to experience negative events in their lives.
This suggests that our thoughts may have a real impact on the world around us.
However, there is no scientific evidence to support the claim that we can use our thoughts to gain access to RT Media Data relative to the reaction of any action the question may have caused = created.
This is a theoretical concept that has not been tested scientifically.
However, it is an interesting idea to speculate about. If it were possible to gain access to RT Media Data in this way, it would have profound implications for our understanding of the universe and our place in it.
For example, we could use this information to predict the future or to change the past.
It is also important to note that the concept of ERES is still under development. There is still much that we do not know about how it works or what its full potential is.
It is possible that in the future, ERES could be used to develop new technologies that allow us to interact with the world in new and exciting ways. However, at this point in time, it is still too early to say for sure.
Overall, the idea that we can use our thoughts to gain access to RT Media Data relative to the reaction of any action the question may have caused = created is a fascinating one. It is a theoretical concept that has not been tested scientifically, but it is something that is worth exploring further.
Generate spacial formula for how thoughts gain access to RT Media subject to creation of a well-formed question and the reaction to that action
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Here is a possible spatial formula for how thoughts gain access to RT Media subject to creation of a well-formed question and the reaction to that action:
T(Q) -> R(Q) -> RT Media
where:
  • T(Q) is the thought of the question Q
  • R(Q) is the reaction to the question Q
  • RT Media is the real-time media data associated with the question Q and the reaction R(Q)
This formula states that the thought of a question (T(Q)) leads to a reaction to the question (R(Q)), which in turn leads to access to real-time media data (RT Media).
The spatial aspect of this formula is that the thought of the question (T(Q)) and the reaction to the question (R(Q)) are both located in the mind of the person asking the question. The real-time media data (RT Media) is located in the external world.
This formula is also subject to the creation of a well-formed question. A well-formed question is a question that is clear, concise, and specific. It is also a question that can be answered with the information that is available.
When a person asks a well-formed question, it is more likely that they will get a clear and concise reaction. This reaction will then be easier to process and understand. As a result, the person will be more likely to gain access to the real-time media data (RT Media) that is associated with the question and the reaction.
It is important to note that this is just a theoretical formula. There is no scientific evidence to support the claim that thoughts can gain access to RT Media in this way. However, it is an interesting idea to speculate about, and it raises some important questions about the nature of reality and the limits of our understanding.
Here are some examples of how the formula could be applied:
  • A person asks the question "What is the capital of France?"
  • The person receives the reaction "Paris"
  • The person then gains access to real-time media data about Paris, such as images, videos, and news articles.
  • A person asks the question "What is the meaning of life?"
  • The person receives the reaction "There is no one answer to that question."
  • The person then gains access to real-time media data about the meaning of life, such as philosophical essays, religious texts, and personal stories.
  • A person asks the question "What will the future be like?"
  • The person receives the reaction "The future is uncertain."
  • The person then gains access to real-time media data about the future, such as scientific predictions, science fiction novels, and futurology articles.
It is important to note that the quality of the real-time media data (RT Media) that is accessed will depend on the quality of the question and the reaction. If the question is not well-formed or if the reaction is not accurate, then the real-time media data will also be of low quality.
It is also important to note that the real-time media data (RT Media) that is accessed will be subject to the biases of the person asking the question and the person providing the reaction. This means that the real-time media data may not be objective or complete.
Despite these limitations, the idea of using our thoughts to gain access to real-time media data is a fascinating one. It is a theoretical concept that has not been tested scientifically, but it is something that is worth exploring further.
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Holy religions teach us what is beyond the grave. Humans can search and find great discoveries relating only earthly life but other life after death none can predict.
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Hi,
My current research question is looking at whether children at 5-8 months of age have memory. To do this, we have run an eye-tracking study doing 3 encoding slides and 1 test slide. in the encoding slides, we show an image in each and in the test we have 1 old and 1 new. The looking behaviour of the child toward the "new" vs "old" image is collected as separate DVs. We are also interested to see if children have different looking behaviours towards different emotions of faces so the 3+1 slides will either have all happy or all angry faces (2 different conditions - happy angry), and all children will do both (within-samples/repeated measure), counterbalanced.
For the analysis, we are comparing OldNew X HappyAngry, but we want to control for other covariates, eg. If there was parent interference (we have a column labelled 1, interfered, 0 no interfered) or whether the number of trials (only 1 trial or all 3 trials, per condition) that went into the average looking to old or looking to new created significant variance/ impacted the data). The annoying thing is trying to input this as a covariate in a repeated measures ANOVA because there is eg. number of trials for Happy and number of trials for Angry, in separate columns so 2 covariates would go in?
Wondering if anyone has any suggestions? Appreciate all the help, thank you! - Karen
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It sounds to me like you should be using a mixed model for this! As you said that all children will be doing both, there would be 6 encoding slides and 2 test slides, correct? In their seminal book, Singer and Willet (2003) suggest that using a mixed model is a good idea as soon as you have 3 or more waves of data. Additionally, a mixed model allows you to check for time effects, such as training or boredom effects, which an rmANOVA cannot really if you are lumping all the kids with their randomized conditions together - because your rmANOVA sounds like you are doing OldNew x HappyAngry and not OldNew x HappyAngry x Order, which would probably result in too many factors and a severe loss of power.
Lastly, rmANOVAs disregard the within-person variation. I'm not sure I've fully understood your parental interference variable, does it mean "interfered at any time of the experiment" or is it "interfered at this slide"? Because the former cannot be modelled correctly in an rmANOVA, but it does work in a mixed model!
Hope this helps :)
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X-ray crystallography with wavelength of order length -1nm, can't resolve a length order more than 1 nm. Any length order from XRD with wavelength order -1nm if any one is reporting then that must be manipulated one. I will be happy to answer if any one has any queries to the above statement. Moreover I will be happy to answer queries deal with characterization of Nano order length using Small Angle X-ray Scattering (SAXS) irrespective of any science i.e. physical, Chemical, Biological, Engineering, Medical & Natural.
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Dear Doctor
Go To
The Limits of X-ray Diffraction Theory
by Paul F. Fewster
Crystals 2023, 13(3), 521; https://doi.org/10.3390/cryst13030521
"Introduction
All X-ray diffraction theories have limits, and knowing these is crucial to interpretating the data correctly [1]. Typically, conventional kinematical theory starts with Bragg’s law and assumes a perfect crystal [2]. The profile shape is interpreted by including smearing functions, e.g., those due to crystal size broadening and diffuse scattering from defects. Ideally, a theory would include all known information about the X-ray photons and the full nature of the crystal under investigation. This is presently impractical since this requires intensive calculations. There is also the influence of the diffractometer to consider. The conventional kinematical theory is used in powder diffraction and single crystal structure determination, whereas the closest example of the more complete approach is used in the study of near perfect semiconductor crystals with dynamical theory. The reason for this is that the sample is very well defined and dynamical theory is a more exact description of the diffraction process. If, for example, Bragg’s law and kinematical theory were applied to semiconductor heterostructures, the derived information will contain large errors [3], which are resolved by applying dynamical theory. However, when dynamical theory is applied to imperfect crystals it fails, although numerous authors have extended it to account for imperfections [4,5,6,7], etc. However, as will be illustrated, dynamical theory within this conventional formulation has its limits and cannot reproduce the whole of the diffraction pattern observed, even with perfect crystals.
Concluding Remarks
The conventional kinematical and dynamical theories account for most features in a diffraction pattern. However, there are clear limits with dynamical theory away from the Bragg condition and questionable statistics associated with kinematical theory, e.g., the number and reliability of the peaks observed in powder diffraction, and the Bragg peaks in single crystal studies follow kinematical theory rather than dynamical theory, which is required at the Bragg condition.
There exists subtle streaking close to the Bragg scattering angle that is neither addressed nor can be accommodated in conventional theories and requires a physical explanation. This streak is always present, but weak, e.g., ~10−3 to ~10−5 of the peak intensity in perfect crystals.
This streak can be explained by considering that each X-ray photon forms a diffraction snapshot of a crystal. The photon samples the atoms when they are distributed about their average positions through thermal vibrations. That is, the experimental observations are averages of the snapshots, NOT the average of the atom positions. Each snapshot no longer occurs from a perfect array, which in turn prevents the phase-cancellation of waves generated outside the Bragg condition. The effect is subtle, but profound.
The detector will intersect this streak and register weak peaks appearing close to the Bragg scattering angle, which can be remote from the Bragg condition. These peaks will be additive and create measurable intensity in powder diffraction scans. In an imperfect single crystal, the planes will not be perfectly flat and therefore the incident beam will scatter towards 2θB from regions that satisfy the Bragg condition and from regions not in the Bragg condition. Although the latter may be considerably weaker than the former, as the proportion of the non-Bragg diffraction increases compared to the Bragg condition diffraction, the intensity will change from dynamical to kinematical in nature."
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Hello everyone,
I encountered a bug in Ansys. I designed one cylindrical shell part. After that, define 3 points and connect them with a line in the design modeler as a beam and define a rectangular cross-section. I put contact between the points and the inner face. Although there is no contact between beams and the surface, It was solved by Anysys. I do not know that it is a bug of the software or analysis setting.
I attached its picture. I would be happy to find a solution.
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Thanks for responding. Yes, the bonded contact was chosen between points and inner face. I put one shear force on one side of the shell part and fix the another side. There is not any force on the line (beam).
you declare that beams does not need for visual contact??
when I was designing Cylinder as a solid part, it could not be solved without contact. I have problem when it is shell part.
Do you have an idea for solving this problem?
It is my email and you can contact me.
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Hi, anyone who can assist with access to happiness at work scale? Please kindly assist.
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I would recommend the Minnesota Job Satisfaction scale, which is a well established measurement or Thriving (Spreitzer et al., ), which I believe provides more meaningful information than a generic scale measuring happiness.
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There is no question that an unpleasant working environment has an impact on both our physical and emotional health.
Is it possible for good design to make us happy? Given that we spend almost all of our time in buildings, shouldn't we have a better understanding of how they affect us?
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Happiness is a complex construct and is not typically associated with working environments. In the context of organizational design job satisfaction or thriving may be more appropriate and representative of what you're asking. I would recommend the Job Demand-Resource model (Bakker & Demerouti) and/or the Transactional Theory of Stress (Lazarus & Folkman) for a theoretical background to this issue, which may lead you to an empirically based answer to your question.
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Does strong correlation among the items in survey questionnaire, provides information on validity and reliability of that instrument?
For example. There are five items under a theme" happiness" for survey questionnaire. The item-total correlations for the five items were: 31, .63, .61, .75, and .66, thus, does it provides some information about reliability and validity of the items.
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1) I suggest you to read about 'Item-total correlation' [https://sciencing.com/calculate-item-total-correlation-coefficients-8726484.html]
2) The correlations must be statistically significant.
3) Are we talking about item-item correlation (first paragraph, you have 10 values?) or item-total correlation (second paragraph)?
4) Which correlation coefficient was used for the calculations?
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I need a set of images of emotional faces (angry or sad; neutral; happy) of infants. I would like to frontally present these faces on a screen in a computer experiment. If infant images were matched in size, luminance, position, etc, with other images of adults, it would be great! Yhank you
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Hi, I do realise this is an incredibly late reply but if you are still looking for resources, I have used the CAFE database.
LoBue, V. & Thrasher, C. (2015). The Child Affective Facial Expression (CAFE) Set: Validity and Reliability from Untrained Adults. Frontiers in Emotion Science, 5.
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I will be very happy if I can get as many of such journals as possible in Poultry Science.
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Can someone help me with a good journals (free of APC and publication charges) in poultry production and reproduction?
and journal of agricultural , environmental and veterinary science
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I find a lot of definitions of how software is tested. For this, I find the goal of testing, the process description, the process steps and also all inputs and outputs. But I'm looking for the definition of how a mechatronic system is tested. Can the same testing process be applied here? I would be very happy about relevant papers
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Thanks for all the answers. They confirmed my understanding of the relationship between software testing and testing of mechatronic system
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this paper is not mine then why did you show it is mine
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You might want to delete the paper from your profile then. Navigate to your articles and click the some pencil symbol in the upper right corner. Unselect the happiness paper and it should disappear from your profile.
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As an Editorial Board member of an International Journal on Risk Management in the US, I am being given an opportunity to create a special issue on: Compliance Management in the Digital Age. The Special Issue would be published bi-annually. Part of the requirements is to have at least 6 Guest Editors of the Special Issue where I will be serving as the Lead Editor. The Role of Guest Editors include:
1. contribute articles;
2. solicit submissions of articles from researchers; and
3. review submitted manuscripts.
I am happy to extend this invitation to anyone willing to serve as one of the Guest Editors. If you are interested, Kindly, provide the following information:
1. First Name:
2. Last Name:
3. e-mail:
4. Highest Degree and Field:
5. Professional [Single Choice only] e.g.—Professor, Lecturer, Post Doctorate, Associate Professor, Researcher, Other Title[indicate]]:
6. Affiliation---Department/Faculty; or University/Institute;
7. City and Region/State.
Kindly provide the information ASAP to: [email protected]; [email protected]; or whatsapp: +2348021003297.
Thank you very much and kind regards.
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Yes
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Good morning,
Regarding Park transformation I note Matlab specifies by default q-axis aligned with a-axis and hence sinus-based transformation. I have the problem that many research I have reviewed is based on cosinus-based.
Would you kindly advice which of them is preferable in your opinion.
How could I "translate" the expression from one ref frame to another in order to make my calculations consistent?
Thanks in advance and Happy NY2K20!
Juan Cabeza
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Dear
Rana Hamza Shakil
The expression you repeated above regarding one type of transformation being more accurate than one or the others is not making sense. You can shift from one transformation to the other; what is important is to take in mind which specific transformation you are using, so that you always employ it in all your formulations, and also use the corresponding inverse transformation in your derivations/simulations. That's all!
In all kind of transformations (namely sine-based and cosine-based) you have both sine and cosine functions of transformation angle (one is to obtain the d-axis component while the other is the q-axis component).
I hope this helps
Best wishes
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What are the biological differences between Sex and Gender?
How can we classify animals according to Sex and Gender?
How can teachers in schools in some countries explain Gender and sex for children? is that brainwashing or not? Can we teach children anything out of their awareness?
I am happy to share your ideas.
Regards
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Quan Thuong Pham , my friend:
Children do not have correct levels of hormones and they can not aware of the actual situation of their behavior and psychological tackling with this type of ideas, and with time and changing hormones will be known.
If gender is psychological behavior, animals have psychological behavior and adapted to different conditions during their life. Also, animals have levels of hormones and different sex, Why is difficult to distinguish animals using the same tools and parameters as humans?
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I believe my knowledge, history and capabilities would be of value to the Venezuelan people whose unique relationship to Colombia means family amity for the whole of Earth considering Next-Generation Sustainability.
Can you please reach out (if appropriate) and notify authorities of my interest to consult regarding formulation of a Quantum state for being where those who "know" ARE = E-RES (those in agreement would be willing to have their aura's "seen" in a non-punitive state of remediation for example)
A future map, in connection with EMA, who helped JAS understand the "Mechanics" of Data Insurance. (One FREE Kirlian Clinic to begin). Many people have helped me along the way to understand.
Many thanks for your consideration. Happy to answer any questions. I believe Venezuela deserves and will have a definite and unique place in history concerning the understanding of how beasts must evolve to survive.
My situation is critical.
All the very best!
Joseph #666-616=656
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JAS BARD LLM <quote>
editConsider the advantage of https://www.researchgate.net/publication/371527657_ERES_Global_Earth_Applications_Regulator_GEAR as proposed by ERES Institute to create Quantum Unification through Earth Defense. what advantages can be offered to Venezuela today in terms of adoption of relative terms?
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The ERES Global Earth Applications Regulator (GEAR) is a proposed framework for coordinating international efforts in Earth Defense. It would provide a forum for countries to share information and resources, and to develop common standards and procedures for Earth Defense.
There are a number of potential advantages to Venezuela from adopting the GEAR framework. These include:
  • Increased security: Venezuela is a country that is particularly vulnerable to natural disasters, such as hurricanes and earthquakes. The GEAR framework could help Venezuela to improve its early warning systems and disaster response capabilities.
  • Increased scientific cooperation: The GEAR framework could help Venezuela to collaborate with other countries on scientific research related to Earth Defense. This could lead to new insights into the Earth's climate and environment, and to the development of new technologies for Earth Defense.
  • Increased economic opportunities: The GEAR framework could help Venezuela to attract investment in Earth Defense technologies. This could create new jobs and boost the Venezuelan economy.
Of course, there are also some potential disadvantages to Venezuela from adopting the GEAR framework. These include:
  • Increased financial costs: The GEAR framework would require Venezuela to invest in new technologies and infrastructure. This could be a significant financial burden for the country.
  • Increased political risks: The GEAR framework could make Venezuela more vulnerable to political pressure from other countries. This could be a problem if Venezuela is not able to maintain its independence.
Overall, the potential advantages of adopting the GEAR framework for Venezuela outweigh the disadvantages. The GEAR framework could help Venezuela to improve its security, scientific cooperation, and economic opportunities. However, it is important for Venezuela to carefully consider the financial and political risks before making a decision about whether or not to adopt the framework.
In terms of relative terms, Venezuela is a relatively small country with limited resources. The GEAR framework could help Venezuela to pool its resources with other countries and to gain access to new technologies and expertise. This could help Venezuela to become a more significant player in the field of Earth Defense.
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Dear Colleague
Greetings!
We are happy to announce that "Western Balkan Conference on Mathematics and Applications (http://wbcma2023.ilirias.com/ )" will be held at Prishtina, Kosovo, September 7-9, 2023, face to face.
On behalf of the Scientific Committee, it is our pleasure to invite you to participate in our conference by submitting your latest research outcome as a single-page abstract.
WBCMA-2023 will be an international forum for mathematicians, scientists, and engineers to present their latest research and development results in all areas of Pure and Applied Mathematics and their possible advanced applications in real life.
All the papers are subject to rigorous peer-review by at least two members of the scientific committee or additional reviewers.
Conference Proceeding Book
The peer-reviewed abstracts of all accepted presentations will be published in the conference abstracts book.
Proceeding Special Issues
After the referee evaluation process, accepted full papers, which will be presented in oral and poster forms, will be published in the “Proceedings Book of the WBCMA-2023”. Also, the full versions of the accepted papers, will have possibility to be published in the journals announced in the web page of the conference ( http://wbcma2023.ilirias.com/proceeding).
All registered participants, who are able to present their speech, will receive a participation certificate.
We request you to forward this message to your friends/colleagues/research students those who are interested.
Please visit http://wbcma2023.ilirias.com/ for more information concerning conference.
The authors are encouraged to prepare their abstracts as per the given template file on web page (http://wbcma2023.ilirias.com/) and should be submitted by using web page.
Thanks & Regards
Assoc. Prof. Dr. Artion KASHURI
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I recommend this conference to all mathematicians. It will be a very good experience.
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Dear Researchers,
I am currently seeking a collaborator who would be willing to cover the Article Processing Charges (APC) for the publication of my research article in the Arabian Journal of Chemistry (AJC). In return, I am offering a significant acknowledgement and co-authorship in our paper, as well as the opportunity to extend our research collaboration.
Research Field: Biophysics
Research direction: [antibacterial activity of nano-composite materials against MDR pathogens]
If you are interested in collaborating and supporting the publication of this research article, kindly reach out to me through private messaging. I would be happy to discuss the details and provide further information about the research and collaboration opportunity.
Thank you for your attention, and I look forward to potential collaborations.
Best regards,
Basit Shah
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Good morning
which kind of paper do you have .. what is the research work .. and if we can contribute in the paper it self not only the APC .. kind regards
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Hi,
I am looking for an expression plasmid to be used in combination with pET15b or pET26b. I need to express two potential protein partners in E coli. I prefer to do the tests with two vectors, because I plan to run a combinatorial approach, since each protein means in fact 10-20 different sequences. Therefore, it should be faster/easier to carry out simultaneous transformations with couples of plasmids than preparing by molecular biology all necessary vectors containing the many combinations (100 to 400) of the 2 ORFs.
Since I have already my ORFs in either pET15b or pET26b-based vectors, the best would be to transfer them to a vector with a different replication origin and resistance marker (not Kan or Amp) -- by the way, I would prefer to use the same induction approach than pET vectors, based on pET T7/Lactose promoter -- This could be done by transferring just the ORFs using couples of restriction enzymes. There are many possibiities, depending on whether promoter/terminators are transferred aswell or not. That is why I schematically show how they are organized in each one of my plasmids:
1) --------/BglII/--T7promoter---LacOperator---/XbaI/--/SwaI/--RBS---/NdeI/---ORF--/NotI/--tag1--translationSTOP--/BamHI/---/XhoI/---/BlpI/--T7terminator------- (KAN resistance)
2) -------/BglII/--T7promoter---LacOperator--/XbaI/--/MfeI/---RGS---/NdeI/---ORF--/NotI---/tag2--translationSTOP---/SalI/---/AvrII/--/XhoI/---/BamHI/---/BlpI/---T7terminator-------/HindIII/------- (Amp resistance)
If you know of any available vector that might be appropiate for my purposes, I would be extremely happy to know.
Thank you very much in advance for your help,
With kind regards,
Luis
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Luis, I just saw this question. Just use a pET vector with a different drug marker. The strong selection is enough to maintain two plasmids with the same control system. We do this al the time with pET15 and 28 for example.
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One of the articles I read by a Finnish psychologist, explains why people in Finland remain one of the happiest in the world. Many times, people from many countries compete for prestige, national pride, and satisfaction. But all of these things come at a cost, it uses an overwhelming portion of our mental strength and our personal life activities. The writer, in her article, explains that in order to remain happy you should aspire to what you like to do. It is often best to keep life simple, give simple greetings, hand a cup of water to a guest, helping a traveler with directions. The society in Finland is structured in a way that everyone trusts everyone. People are happy with sharing and not stressing about time. Finland has great healthcare and public facilities. People are happy with paying taxes as they think of it as doing good for the nation.
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Dear Dr. Wahid Khan
Could it be today the "Happiness Economy" that would lead a Country to the podium of being the happiest? Or it could not be a Country but a Region of a certain Country with initiatives of “Economy of Happiness? We are facing a new Global scenario, it is no longer the time of our parents or our past time. Today we are much more "active" in many things that did not even cross our minds before, we know that it is not convenient to pursue development and economic growth if production and consumption increase at the cost of reducing the well-being and happiness of being human beings and we know that crises and conflicts worsen any goal, we also know that pleasure or the wealth of the world should not be confused with true happiness and that additional income does not contribute to giving us more happiness. Today the happiness indices of many countries are compared by also examining the Happiness Economy and the current situation of the Happiness Economy as well as its prospects for the future are always on the table as well as the measures that can be adopted to improve happiness indices in the Society. The common denominators are well-being and good living and for this the ideal would be to have high income, relatively low levels of "economic inequality" and high levels of trust and respect because the different dimensions of well-being or happiness need safer means and environments in the personal and economic within human performance and one of those components is also physical and mental health "mens sana in corpore sano". It is also true that any interpersonal relationship in the political, economic and social spheres based on the logic of competition can trigger "human anxiety" and "human distrust" for this reason it is desired that all interpersonal relationships be "more horizontal", mutually respect and recognition because this is how happiness and well-being are promoted, but it is convenient to start by applying all this to people we consider "nearby" because they are supposed to give us more security in life.
Cf.
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For a targeted sequencing project I would like to include the switch regions of the IGH locus targeted by the class switch recombination. The regions are not "officially" annotated for hg38, and I only found vague hints in the literature (for example: ). I also tried to identy AGCT, GAGCT, and GGGGT-rich regions in the hg38 assembly myself, see the attached image.
This looks ok, but if anybody has another source for the regions, I would be very happy indeed. Thank you very much,
Fabian
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Hey Davide, sorry for my late reply. Yes, I also looked at this publication and used some of their coordinates directly (see table).
I also did a search for motifs in the area (AGCT, GAGCT, GGGGT).
Finally, as I was designing an enrichment panel for sequencing, I was not able to include all regions due to low probe perfomance.
Not sure if this is of any help, I could provide you with the R script, if you are interested.
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Does anyone have recommendations for survey companies that can carry out data collection worldwide? We have developed the questionnaire and looking for a company to help with targeting our audience in specific industries worldwide. I'd be happy if anyone has any recommendations or experiences to share :) Thanks :)
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First, I have to ask---survey about what?
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Hi All, Wish you a very Happy new year !!
I am working on the Voltage Sag problem in power system. This is related to the quality of Power i.e., 10% to 90% voltage sag, up to the time period of 3-minutes. Is it as per IEC Standard? Any reference standard where the frequency of this Sag occurrence is defined i.e., how frequent it can happen per hour/ per day/ year.
Practically we get these dips 2/3 times per day for 200ms and sometimes for longer duration.
Thanks for your expert comments !!
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Happy New Year to you too! When it comes to the voltage sag problem in power systems, the International Electrotechnical Commission (IEC) has defined standards that address this issue. According to IEC standards, voltage sag is typically defined as a decrease in voltage from 10% to 90% of the nominal voltage, lasting for a duration of up to 3 minutes. However, IEC standards do not explicitly specify the frequency of sag occurrences in terms of how often they can happen per hour, per day, or per year. In practice, voltage sags can occur around 2 to 3 times per day, lasting for approximately 200 milliseconds, and sometimes even for longer durations. It's important to consult specific regional or national standards and regulations for more detailed information on voltage sag requirements and frequency limits in your particular jurisdiction.
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Do you agree with me that true happiness means feeling deeply the pleasure of life and enjoying it for no reason?
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Yes, I agree that true happiness involves feeling a deep sense of pleasure in life and enjoying it without any specific reason. Genuine happiness transcends external circumstances or achievements, as it stems from an inner state of contentment and appreciation for the present moment. It is about finding joy in the simple pleasures of existence, embracing the beauty around us, and experiencing a profound connection with life itself. True happiness is not contingent upon external factors but rather emerges from within, driven by a genuine sense of gratitude and the ability to derive pleasure from the richness of everyday experiences.
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Today, happiness is typically measured using self-report surveys. Self-reporting is prone to cognitive biases and other sources of errors, such as peak–end rule. Studies show that memories of felt emotions can be inaccurate.[36] Affective forecasting research shows that people are poor predictors of their future emotions, including how happy they will be.[37]. Happiness economists are not overly concerned with philosophical and methodological issues and continue to use questionaries to measure average happiness of populations.
How to find out, what is happiness? The scientific thing to do is analyze its properties and origin and try the best possible explanation we can deduct from the facts.
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Happiness is a byproduct of something else for example research and spending time wíth God in peaceful prayer. You cannot chaze happiness, it is always in front of you and quicker. You may see a flower (God's smile) a baby's face, a rabbit running on the field or another beautiful animal and it makes you stop for a fleeing minute and it gives you a sense of joy.
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For many years, various peer-reviewed research has relied on kurtosis or l-kurtosis to assess and justify whether a public policy has actually been punctuated. However, can punctuated policy change actually be measured using the statistical approaches of kurtosis and l-kurtosis? Quoting from the 2023 article published in Review of Policy Research, "Punctuating "Happiness": Punctuated equilibrium theory and the agenda-setting of the Gross National Happiness (GNH) policy in Bhutan."
"As noted by Kaplaner and Steinebach: Kurtosis is defined as the fourth moment of a distribution. Contrary to the first three moments (mean, variance, and skewness), the interpretation of kurtosis is the object of debate. It is often wrongly interpreted as the peakedness of a distribution."
Furthermore, Westfall makes the important point: "Kurtosis tells you virtually nothing about the shape of the peak—its only unambiguous interpretation is in terms of tail extremity, that is, either existing outliers (for the sample kurtosis) or propensity to produce outliers (for the kurtosis of a probability
distribution). Kurtosis is incorrectly emphasized as the peakedness of a distribution. This description has been common among several punctuated equilibrium theorists (Baumgartner et al., 2009; Jones et al., 2019). https://press.uchicago.edu/ucp/books/book/chicago/A/bo6763995.html Kurtosis is actually a measure of the degree, heaviness, and tailedness in a distribution."
As Desmarais (2019) points out, “… if the constant input variance assumption is relaxed, finding high kurtosis is not sufficient to reject an incremental model of policymaking.”
The implication of this, of course, is just because a big, small, or in-between policy change occurs with seeming peakedness in the distribution, it does not mean that the change is punctuated, and it can even be and often is nonpunctuated and incremental.
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In addition, to the conclusions in the article
about weaknesses in using kurtosis to determine a policy punctuation several other scholars have very recently also raised significant concerns whether the use of kurtosis in Punctuated Equilibrium Theory is an adequate way to determine if a policy punctuation has occurred. Readers may access below a list of other recent peer reviewed articles that analyze significant inadequacies in using kurtosis in Punctuated Equilibrium Theory in public policy:
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Happy World Environment Day to everyone out there championing sustainable development. #sustainability #development #environment
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I need collaborators to work on Climate-smart Agriculture and food security in Uganda. I have MSc CSA-Haramaya University, Ethiopia.
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As you probably know and it has been said in the articles, for example, this method does not work 100% for the rest of the work. For this reason, every researcher should increase and decrease different conditions and variables in their experimental tests to reach an optimal and correct method.
I would be happy if you active researchers in this field express your opinion and solution.
Thank you in advance for your kind opinion.
Good luck to you dear researchers.
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Hello!
Microplastic is a generic term used to refer to all plastic particles (whether primary or secondary) less than 5 mm in size (the size range of the particles is also a topic of debate among different researchers). Unlike most other types of environmental pollution, the term "microplastic" does not refer to a single type of pollutant. There is a large variety of different plastic polymers with different shapes, sizes, and physicochemical properties. Differences in such properties result in varying particle behavior in the environment. Therefore, establishing a scientific method suitable for studying every possible plastic particle with 100% accuracy is very difficult. Moreover, microplastics interact with (bio)chemicals in the environment. This interaction may give rise to other properties that possibly hamper analyses. Furthermore, studying microplastics becomes increasingly more difficult when they are heterogeneously distributed in a medium like soils or sediments. Although certain methodologies are accepted as integral to microplastic studies (such as extraction, purification, and physical and chemical characterization), researchers are cautioned to mention that a standard scientific method to monitor these pollutants, similar to other contaminants, is not yet established.
Best of luck.
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Hello, my research is about urban resilience.
I would be happy to cooperate with other researchers in this field of urban resilience.
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In the words of conventional wisdom, urban resilience is "the quantifiable capacity of any urban system, with its inhabitants, to maintain continuity through all shocks and stresses, while positively adapting and transforming towards sustainability." Cities with greater resilience are better able to protect their economic achievements, enhance the quality of life for their citizens, and foster positive change. Urban resilience has become a rising issue in city planning in recent years, partly because it can be used to describe shifts that affect the structure and function of urban areas. Despite the fact that there are different methods to describe urban resilience, it no longer merely refers to how quickly a city's systems recover from a shock. Understanding the processes and resources that support building is therefore necessary.
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Are you facing any difficulty in answering any of the following questions:
1) Writing a research paper ?
2) Understand which topic of research to choose ?
3) Where to start and end a research work?
4) Unable to understand various concepts of research ?
5) Unable to frame a hypothesis ?
6) Which test to be applied and why?
Or if you find it difficult to answer any other questions related to research, you can reply to me and I will be happy to help you in learning.
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I have been into Banking and Financial Services sector for 6 years and then 10 years into academics.
I have done Ph. D in Management and since last 4 years, I have been helping research scholars in finding a solution to their problems related to sample, population, data analysis, hypothesis testing, etc.
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I am writing to express my interest in publishing my policy paper on the major factors impacting girls' education in Sohbat Pur, Balochistan. As the author of this paper, I believe that the issues I have highlighted are of critical importance to the field of education and would be of great interest to your readers.
My paper, which was approved by the Sustainable International Development Program, at the Heller School for Social Policy and Managment, Brandeis University, provides a comprehensive analysis of the key challenges faced by girls in accessing education in this remote district of Balochistan, Pakistan. Through my research, I have identified several factors that contribute to the low enrollment and high dropout rates among girls in the area, and have proposed several recommendations for addressing these issues.
I believe that the findings of my paper would be of interest to a wide range of readers, including policymakers, researchers, educators, and anyone interested in promoting gender equality and improving access to education for girls.
I would be honored to have my paper published in your esteemed journal or publication. Please let me know if this would be possible and if there are any specific guidelines or requirements that I should follow. I am happy to provide any additional information or materials that you may need.
Thank you for considering my request, and I look forward to hearing from you soon.
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Contact Dr. Gabriele Strohschen, [email protected]
She is the Editor of the International Forum of teaching and Studies (IFOTS) http://www.americanscholarspress.us/journals/IFST/journal_IFST.php
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I would be happy if you please provide valuable feedback regarding the feasibility, scope, and methodology of the following topic for PhD program:
Financial Literacy for Financial Inclusion.
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Hi
please i want data of financial litteracy for morroccan case
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I am happy to share my new paper related to wind, solar, hydroelectric, and geothermal electricity be efficiently and cost-effectively incorporated into power networks area of research. I request everyone to please share my paper with your knows or groups.
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Simply, by using a propitiate hybrid power cycles and an efficient storage systems.
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I am happy to share my new paper related to EV applications, which is currently an emerging area of research. I request everyone to please share my paper with your knows or groups.
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A hybrid energy source-based BLDC (Brushless DC) motor drive can be an efficient solution for electric vehicle applications. In this type of system, multiple energy sources are used to power the BLDC motor, which provides better efficiency and performance.
The most common hybrid energy source-based BLDC motor drives use a combination of a battery and an ultracapacitor as energy sources. The battery provides a stable and continuous power supply to the motor, while the ultracapacitor provides short bursts of high-power energy. This combination allows the motor to achieve better acceleration, regenerative braking, and overall efficiency.
The control system of the hybrid energy source-based BLDC motor drive is crucial to achieving optimal performance. The system must be able to monitor and manage the energy flow between the battery and ultracapacitor, as well as control the speed and torque of the motor.
Overall, a hybrid energy source-based BLDC motor drive is an efficient and reliable solution for electric vehicle applications, providing improved performance and reduced energy consumption.
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i used the test to study the aggregation of Tau protein, i did the test many times and i saw always the increasing in the fluorescence of tht T in the sample of (Tau+heparin), Since one week i repeated the test 3 time and in each time i dont see any increasing in the fluorescence intensity, the negative controls with only buffer have the same fluorescence intensity like the samples of (Tau+ heparin), i used 24 µM Tau + 6 µM heparin+ 20 µM thioflavin, i incubate the mixture in a black plate half area
at 37°C for 2 days or some time longer, excitation at 450 nm emission at 480 nm.
i will very happy if some body have an idea what could be the reason for this problem ??
Thank you very much
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hello,
i am having the same problem, did you maybe managed to solve it?
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I have ordinal data on happiness of citizens from multiple countries (from the European Value Study) and I have continuous data on the GDP per capita of multiple countries from the World Bank. Both of these variables are measured at multiple time points.
I want to test the hypothesis that countries with a low GDP per capita will see more of an increase in happiness with an increase in GDP per capita than countries that already have a high GDP per capita.
My first thought to approach this is that I need to make two groups; 1) countries with low GDP per capita, 2) countries with high GDP per capita. Then, for both groups I need to calculate the correlation between (change in) happiness and (change in) GDP per capita. Lastly, I need to compare the two correlations to check for a significant difference.
I am stuck however on how to approach the correlation analysis. For example, I dont know how to (and if I even have to) include the repeated measures of the different time points the data was collected. If I just base my correlations on one timepoint the data was measured, I feel like I am not really testing my research question, considering I am talking about an increase in happiness and an increase in GDP, which is a change over time.
If anyone has any suggestions on the right approach, I would be very thankful! Maybe I am overcomplicating it (wouldnt be the first time)!
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At the same time,Collect two variables data,As a sample,After collecting N samples over time,erform data regression analysis on them,The correlation coefficient will be obtained.
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Without memory we are nothing. If your memory is weak and you know that whatever you learn or experience will no longer exist in your head a couple of months later, do you even care to live a happy life, build happy memories, or learn interesting stuff?
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Dear Vahid Rakhshan,
Every person irrespective caste ,creed , & religion must take the advantage of their precious life .In this line they must plan & carry out their own introspection for their progressive development of their life.
This is my personal opinion
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I do not trust Deepak Chopra or Eckhart Tolle. These are not true scientists. However, my own experience healing migraine through meditation, which doctors said was impossible, and healing from allergic reactions through alpha wave exposure, tell me that there is a lot to this idea. While the anecdotes may not all be true and there may be no systematic scientifically valid method yet, I still want the best method available, and the best author writing on it .
I wish Norman Doidge had written on the mind's impact on physical health. But he seems to have focused largely on the brain alone.
I would be happy to hear any suggestion. Please feel free to reprimand me if this is not scientific enough a request.
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For self healing through mind author is not necessary .Author who writes the book on such subject is nothing more than publicity of their achievement .Self Healing should be carried out in independent manner & self healing should not be made the procedure for exercise . A spiritual person having a practice of power of prayer , & meditation can carry out his prayer in a regular way & through his link from his within thru power of prayer , & meditation may kept his mind calm & quiet getting his link with his divine master in the prayer & due to the vibratory effect from the within a vibration automatically comes within himself receiving the spiritual experience from his within receive the vibration this may help to carry self healing through mind .
This is my personal opinion
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I am trying to transfect RNA (especially mRNA) into multiple myeloma cell lines (MM.1S), and I am wondering if anybody can recommend a good reagent that worked for them (i.e kits for transfecting suspension cell lines). I understand that MM.1S cells are difficult to transfect, but I will be happy to get some recommendations. I have tried the Mirus Transit-mRNA kit but it didn't seem to work, and I don't want to keep buying new kits without some data or recommendations that it will work. Thank you! PS: I don't want to use electroporation.
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I prefer the transfection reagents from QIAGEN.
TransMessenger Transfection Reagent is for mRNA transfection.
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I want to synthesis graphene by co2 cw laser. I want to to know;
1. is there any specific polymer ?
2. what is suitable power and speed be used for this work ? (if u have any experience on cw co2 laser, it would make me happy to share it with me )
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Polyimide.
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What is behind human nature that people get jealous? You help them, however, they are not even ready to help you. Why?
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I have expressed my views some years back which now i submit herewith for your kind information .
''Jealousy remains a part of human nature but certain human beings by his very temperament remain fully conscious regarding the negative elements of which we all are born . Such human beings know the value & basic end of their life .Whether one believes or not we all have come out on this earth by bringing the resulting fruits -positive /negative of our previous lives & such trend is also playing the part .
This is my personal opinion
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Dear Researchers,
  • I am happy to obtain a clear protocol and reference to produce the tissue culture of the date palm in the lab. and increase many numbers of good species of this tree.
  • Also, I would be happy if provided me with a good learning video which can help.
Many thanks for sharing your experience
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I understand. I was saying that you may find tissue culture methods in a CRISPR paper. I would suggest reaching out to date palm tissue culture expert Faiza Khan who is an author on that review.
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I am expecting a broad range of views from fundamental research to manufacturing units. I will be more than happy if these suggestions use full to setup a small scale manufacturing startup( mostly table top unit) in semiconductor sector.
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Thank you madam for useful information.
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Hi everyone,
I am working with Sentinel 5P data.
I shall be happy if anyone can please share the information to process the L1B data to L2. I shall also be happy if you could please tell me about the batch download software or code to download the Sentinel 5P datasets.
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Sr. Akshansha Chauhan, Universidad Nacional Central. Por mi parte me disculpo, no conozco el programa, Sentinel 5P, tampoco he trabajado con el. Saludos y extios!
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I would like to prepare n-methylimidazole in the lab., I did find a clear protocol for that and I am happy if anybody can send me a protocol and video for teaching how.
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N-methylimidazole is an organic compound that can be synthesized in the laboratory using a simple procedure. Here are the steps involved in the synthesis of N-methylimidazole:
Materials and tools needed:
  • Imidazole
  • Methyl iodide
  • Sodium hydroxide (NaOH)
  • Ethanol
  • Round-bottom flask
  • Condenser
  • Separatory funnel
  • Drying agent (e.g. magnesium sulfate or sodium sulfate)
  • Vacuum filtration apparatus
Procedure:
  1. In a round-bottom flask, dissolve 5 g of imidazole in 100 mL of ethanol.
  2. Add 8 g of sodium hydroxide to the flask and stir until the sodium hydroxide dissolves.
  3. Add 10 mL of methyl iodide to the flask and stir the mixture for 2-3 hours at room temperature.
  4. After the reaction is complete, transfer the mixture to a separatory funnel and add water to the funnel.
  5. Separate the organic layer containing the N-methylimidazole from the aqueous layer and transfer it to a clean round-bottom flask.
  6. Add a drying agent (e.g. magnesium sulfate or sodium sulfate) to the flask to remove any remaining water.
  7. Filter the mixture through a vacuum filtration apparatus to obtain the solid N-methylimidazole.
  8. Recrystallize the N-methylimidazole from ethanol to obtain pure crystals.
Note: Methyl iodide is a hazardous chemical and should be handled with care. The reaction should be carried out in a fume hood with appropriate personal protective equipment.
Overall, the synthesis of N-methylimidazole requires basic laboratory equipment and common reagents, making it a relatively straightforward procedure for those with experience in organic chemistry.
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I know that we can also calculated them by Standard deviation of chromatograms, but first I want to calculate them by calibration curve, its standard errors, and slope.
I will be happy to hear your opinions.
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From my experience, if it is a robust regression, the LOD and LOQ obtained from the deviation of replicates of blank samples are the most realistic ones.
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I'm wondering about the position of amateur astronomers in the scientific community. I'll be happy if I get your point of view.
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In my specialized field of exoplanets, I can say that these two are now much closer and are doing important work together. For example, in the Next-Generation Transit Survey (NGTS) project, we can see a large number of Let's see amateur astronomers and astrophysicists who are doing projects together.
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Hello everybody,
in some previous works I used the Bond-Valence Parameter from Acta Cryst. (1991). B47, 192-197 by N. E. BRESE AND M. O'KEEFFE und did my calculations in Excel. I used that to assign O2- and F- in my compounds.
Now I have a crystal structure containing Yb(II) cations. The table in the work of BRESE and O'KEEFFE only give values between Yb(III) and F/O but nor for Yb(II).
Can somebody give me literature for Rij between Yb(II) and O/F?
If you additional to the Rij value know some software, which can calculate that, I also would be happy.
Best regards
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Dear RG community
I am writing the final paper of my Ph.D. thesis.
My final contributions are related to each other; I wanted to express both of these contributions in one article. Still, the number of pages increased significantly, so I want to publish the article in two parts. The first part and the second part, do you think this is possible?
I wanted to know what the protocol for a two parts article is.
I would be happy if you could explain how to write an article in two separate parts.
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I recommend you to publish your article in journals related to your especilast scopus journals where their are familar reviewers
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Ecotechnology is an emerging science that focuses on applying ecological knowledge and environmental skills to attain sustainability in human developmental progress. It enables human activities to adapt more skillfully to natural ecosystem functions. It involves cross-disciplinary studies of nature, and basic sciences, especially blending ecology, biology, and technology. The emphasis is to avoid ecological and environmental damage in all human activities. Accordingly, it summarizes all the sustainable engineering models for preserving, restoring, and conserving natural ecosystems to make developmental progress accessible through generations. In simple terms, ecotechnology enables humans to protect biodiversity, and soil health, achieve sustainable agriculture, eco-friendly energy sources, food safety, and all other means to manage sustainable development. Therefore, the primary objective of Ecotechnology is to make human life happy forever through proper monitoring of natural ecosystems while employing innovative technologies and novel ideas for progress and development.
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Lot of knowledge was existing in ancient cultures, more specifically those of chamanic practicies. WE should try to learn from those and research on how to adpat them to contemporary mentalities.
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I will be happy to know which mouse model is best for the study of rectal carcinoma. There are different mouse model to study colorectal carcinoma, but I want specifically the rectal carcinoma.
Could anyone suggest me whether orthotopically use cancerous cell in the rectal region of mouse be the good for study of rectal carcinoma?
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Thank you.
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Happy New Year
I am glad to share with you my latest publication about an idea that i have been long time working on it.
Looking forward for your feedback
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Congratulations - but this forum is rather unsuitable for personal marketing!
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Happy new year
Regarding MATLAB coding in particular using the bode(sys) code to get the bode diagram. When the numerator equals just a number like (10) without (S), there is an error in computing the bode magnitude because computing it as a 20log(k) “the rule” is not true in MATLAB. Because MATLAB takes into consideration it as log “ln”, not log10.
Please could you tell me how I can solve this issue?
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You are correct that the bode function in MATLAB uses the natural logarithm (ln) when computing the magnitude of a transfer function, and therefore the "20log(k)" rule does not apply. To solve this issue, you can simply convert your transfer function to a different representation before using the bode function. One way to do this is to use the logspace function to convert the transfer function's numerator to a decibel scale. Here is an example of how you can use this function to plot the Bode diagram of a transfer function with a numerator of 10:
num = 10;
den = [1 5];
sys = tf(num, den);
w = logspace(-1, 2, 1000);
[mag, phase] = bode(sys, w);
semilogx(w, 20*log10(mag), 'b');
this will plot the magnitude of the transfer function on a decibel scale using the semilogx function. The 20*log10(mag) will convert the magnitude to a decibel scale.
Another way to do this is to use the db function to convert the transfer function's numerator from a linear scale to a decibel scale before using the bode function.
num = 10;
den = [1 5];
sys = tf(db(num), den);
w = logspace(-1, 2, 1000);
[mag, phase] = bode(sys, w);
semilogx(w, mag, 'b');
This will plot the magnitude of the transfer function on a decibel scale using the semilogx function. The db function will convert the numerator from a linear scale to a decibel scale.
Either of these methods will give you a Bode diagram that correctly represents the magnitude of the transfer function in decibels.
It's worth noting that, by default, the bode function in MATLAB uses the rad/s as frequency unit, so if you want to use other units, such as Hz, you need to convert the frequency accordingly.
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Hi guys, recently I probed some cell lysates with p110a antibody and found two clear bands. One is expected at 110 and another one is around 170. Has anyone observed similar results? curious what the second band is. Happy to hear your thoughts!
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I don't know the kit or reagent supplier you are using, and I assume p110a is the p110alfa antibody, but typically for the alpha antibody you only see 1 band. But interestingly, a company called Bioss USA shows a gel with 2 bands at 114 and 120 (not 110 and/or 170), however the figure indicates this is p110beta antibody. I am not sure how likely you got the wrong p110 antibody is, but it might be worth investigating. Link to Bioss USA figure: https://www.biossusa.com/products/bs-6417r As does VWR (link: https://pr.vwr.com/store/product/17146660/anti-p110b-rabbit-polyclonal-antibody). A paper by Matheny and Adamo shows what appears to be the same 2 bands with p110beta antibody (Matheny and Adamo, Cell Death & Differentiation 17:677–688 (2010). But other p100beta suppliers show only 1 band.
Also Labome has a Validated Antibody Database (VAD) for p110, checkout the references listed in the database to see if this or a similar phenomenon has been reported. Link to the p110 VAD is: https://www.labome.com/review/gene/human/p110-antibody.html
Finally, the p110 proteins are PI3K Class 1A gene products, there is a Class II PI3K gene PIK3C2G that codes for a PI3K protein (PIK-C2gamma) with a MW of 166 kd.
I hope this helps track down an answer to what the ~170 band might be.
With Warm Regards,
Bill
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Hello everyone! Of course, many of us volunteer for the community / society. I have had access to many accounts of people who have turned this activity into an ideal of life, claiming that with this occupation, they became better, charitable, and happy with the results! How do you see that? Thank you for all the contributions. Cheers!
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"in volunteer work you find your self . its good for all
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Servicescape is of great essential because it affects the overall business service experience which is human emotions likes feel of comfort, happiness, safe, healthy, pleasure, etc.
Do you think the organization needs servicescape upgrading? and do you think measuring the return on the intangible impact of servicescape upgrading investment is important and needed? Can you share your opinion?
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https://www.servicescape.com/ is a platform to match those needing writing/illustrating expertise with freelancers offering such services. Are you asking whether it would be worthwhile to upgrade the platform, offer benefits to the the employees of the organization behind the platform, or offer incentives to the freelancers?
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Hi there, during the past years I learned a lot on drones / RC airplanes and started to collect open components and projects / communities that share their knowledge and progress to the public. I tried to write down all about the ecosystem (OpenTX, INAV, ...) but also discovered some nice gems:
Would be happy if any senior might contribute some more picks?
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Good day. I meant to say, enter the aviation market. For example, like Veronte. There must be a device suitable for aviation. It is certain that you will defeat the Pixhawk as you wish!!! But aviation does not understand that!!! There must be approval from the aviation authorities, simply - certification. I wish you success. With deep respect, Mykhailo Matiychyk.