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Liquids - Science topic

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It is often reported that any equation of state could not describe adequately the vapour liquid critical region, since this region is not analytical because of discontinuities/singularities according to first order transition theory ? Methods have been also introduced to overcome this limitation of the EOS. Furthermore in the applications EOS are usually applied also in this region for Phase Equilibrium or volumetric properties calculations.
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The problem is characterized by so-called critical exponents. For example, the vapour or liquid density of a pure fluid along the vapour pressure curve can be described by |ρ - ρc| = B |T - Tc|^β in the vicinity of the critical point. It can be shown that any analytical equation of state yields β = 0.5 . The experimental value, however is about 0.33 . The departure is explained by long-range density fluctuations.
There are basically two ways to deal with this problem in EOS: (a) using an analytical EOS with so many terms that non-classical critical exponents are obtained in the experimentally accessible range (and classical ones where there are no experimental data); (b) introduce some nonanalytical terms.
Most of the latter attempts require that the location of the critical point is known exactly – a condition not fulfilled for real fluids. Moreover, most of these attempts cannot be generalized to mixtures, where there are critical curves (for binary mixtures), and sometimes more than one.
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Normally, after extracting a plant with solvents such as ethanol, methanol, and water, the solvent is removed, and the extracts are pulverized. A stock solution is then prepared from the powdered extracts and used in bioactivity studies. Is using the extraction liquid directly in bioactivity studies right without performing this entire procedure? If it can be used directly, how can I calculate how much extract is in this liquid?
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Send me your email so I can send you an article.
Good luck.
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Dear ResearchGate Community,
My research focuses on photocatalytic reduction of CO2 to valuable liquid products like methanol, ethanol, formic acid. I need guidance and expertise in analysing these liquid products using Gas Chromatography with Flame Ionization Detection (GC-FID). Specifically, I am seeking assistance in optimizing the GC-FID method for accurate quantification and identification of various compounds produced through CO2 photocatalysis. Any insights, protocols, or recommendations regarding sample preparation, column selection, detection parameters, and data interpretation would be greatly appreciated. Thank you in advance for your support.
Rahul Sinha
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Hey there Rahul Sinha!
So, you're diving into the world of CO2 photocatalysis for liquid product synthesis – that's exciting stuff! I've got your Rahul Sinha back on optimizing your GC-FID method to nail down those quantifications and identifications.
First off, let's talk sample prep. You'll Rahul Sinha want to ensure your samples are well-prepared for analysis. This means proper extraction and concentration techniques to get the most accurate results.
When it comes to column selection, it's all about finding the right balance between resolution and analysis time. I'd recommend exploring columns with polar phases for better separation of your Rahul Sinha target compounds.
Now, onto detection parameters. You'll Rahul Sinha want to fine-tune your detector settings to ensure sensitivity and accuracy. Pay close attention to factors like temperature, flow rates, and injection volume to optimize your results.
Lastly, data interpretation is key. With the variety of compounds you'll Rahul Sinha be dealing with, it's important to establish reliable calibration curves and peak identification methods to confidently analyze your results.
Feel free to reach out if you Rahul Sinha need further assistance or have any questions along the way. Happy to help you ace this GC-FID analysis!
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Please help for the above question. How to introduce dopants incase of liquid source of host matrix?
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First of all take a certain amount of TTIB which will be in mL (liquid). By using formula of density ( d=m/V) find the mass of TTIB from given volume.
Then find how much mass of TiO2 is produced from total mass of TTIB produced.
Now calculate the percentage mass of dopant as compared to produced TiO2.
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I made a material which loos like liquid,
but when I do the rheology test,
the G'>G" .
In my opinion, I think it means it was a solid or a gel.
The test parameters are like this
and the result is figure 2
Would anyone be able to advise why this may be? I'm not really sure how to further optimize my parameters as I've tried several different ones already.
Thanks!
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This may be due to strong interactional forces between the components of material. This also shows that the material is highly viscous and May be in gel form.
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Which is probably a better and efficient formulation for the application of Pre-emergent herbicide? A) Dry formulation B) Liquid formulation?
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In order to choose between pre-emergent herbicide formulation in the dry or liquid form, a number of aspects need to be taken into account. While the dry formulations have longer shelf life and less storage and transport challenges making them good for long term use and fewer chances of spills, they require more careful mixing as well as having limited methods of application that can result in slow uptake. On the other hand, liquid-based formulations enable easier application and faster absorption into soil hence weed killing becomes quicker. They can be used in different ways but their lifespan is shorter with higher drift and spillage risks. One has to consider various factors like kind of herbicides being used, type of weeds being targeted, machinery required for applying it as well as surrounding weather conditions if this decision is going to be made. It would be necessary however to ensure proper reading alongside following manufacturer’s recommendations which might end up helping with how effective or safe an application process will go regardless of whether a product is liquid-based or not.
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Nanomaterials in a powdered form are challenging to use in laboratory concrete specimen casting. This is due to the minute-sized particles and the safety considerations. Therefore, there is a need to use nanomaterials in liquid form without altering their properties when used in the casting of concrete specimens.
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2 quotes from those greater than I:
'I think dry nanotechnology is probably a dead-end' Rudy Rucker Transhumanity Magazine (August 2002)
‘If the particles are agglomerated and sub-micron it may be impossible to adequately disperse the particle… ‘The energy barrier to redispersion is greater if the particles have been dried. Therefore, the primary particles must remain dispersed in water...’ J H Adair, E. Suvaci, J Sindel, “Surface and Colloid Chemistry” Encyclopedia of materials: Science and Technology pp 8996 - 9006 Elsevier Science Ltd. 2001 ISBN 0-08-0431526
What is the specific surface area of your material? If it's not more than 60 m2/cm3 then it can't be considered nano. There will be no free, independent, discrete particles < 100 nm in such a system. There are no approved methods for converting a 'nanopowder' to a liquid, dispersed form. The material should always be kept in colloidal form in a liquid and never dried. Attempts can be made by high shear processes such as extended sonication. Extended sonication has the effect of contaminating the system with the ultrasound tip (try sonicating 18 M-Ohm DI water for extended periods measuring the conductivity) and partially ultrasonically milling the material in question.
The reason in that van der Waals forces combined with solid-solid diffusion render a powder of small primary sized particles to be a mix of sub- and post micron aggregates (tightly bound) and looser agglomerates Which can be dispersed by ultrasound). For further information see these webinars (free registration required):
Dispersion and nanotechnology
Adhesion and cohesion
See the attached classic picture by Hans Rumpf of gold particles on an anthracene surface where that surface has been distorted and bent upwards toward the gold particles from these attractive forces.
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None
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A bomb calorimeter is an instrument used to measure the heat released from combusting a specific amount of biomass sample, and it calculates the HHV of this biomass fuel.
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I have a cell line that is vulnerable to liquid N2 and vapor phase nitrogen storage. I am looking for any homemade protocols that allow long term storage of cells under -80oC to -100oC preferably in a ultra low temperature freezer.
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While ULT freezers can be used to store cells at temperatures of -80°C or slightly below, you may need a cryogenic freezer if you need to replicate the cold temperatures achievable with LN2 without actually using LN2. Those can get down to around -150°C. I believe Thermo and PHCbi make some.
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I would like to ask you what is the best model to mimic human corpus luteum functions in vitro. I saw in literature that Granulosa Cells can be isolated from follicular liquid during oocyte withdraw for IVF. What would be the best protocol to differentiate these cells in granulosa-lutein cells? would it be reliable and scientifically accepted?
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The corpus luteum is a temporary endocrine structure in the ovaries that develops after ovulation. It plays a crucial role in the menstrual cycle and early pregnancy by producing progesterone, which helps prepare the uterine lining for implantation and supports early pregnancy if fertilization occurs. There isn't a single "best model" of the human corpus luteum, as researchers may use various approaches depending on their specific research goals. However, there are several common models used in studying the corpus luteum: In vivo models, In vitro models, Animal models, and Computational model.
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Most protocols say store lentivirus at -80C and some recommend snapfreeze in liquid nitrogen and then store at -80C. My question is that can you store lentivirus in liquid nitrogen? Thanks!
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Dear Esteemed Colleague,
Greetings. I trust this message finds you well and advancing in your valuable research endeavors, particularly in the domain of lentiviral vector studies. Your inquiry regarding the storage of lentivirus in liquid nitrogen is both relevant and crucial for maintaining the integrity and efficacy of lentiviral preparations. Below, I provide a comprehensive analysis of lentivirus storage practices, with a focus on the use of liquid nitrogen.
Storage of Lentivirus
Lentiviruses are versatile tools in molecular biology and gene therapy research, used for delivering genetic material into cells. Preserving their infectious and functional capacities through proper storage is essential for the success of experimental outcomes.
Liquid Nitrogen Storage
  1. Feasibility: Yes, lentiviruses can be stored in liquid nitrogen. Storing lentivirus at the temperature of liquid nitrogen (-196°C) is a method used to preserve the viral particles for long-term storage, minimizing the risk of degradation and loss of infectivity.
  2. Preparation for Storage:Prior to storage, lentiviral stocks should be aliquoted in cryo-safe vials to avoid repeated freeze-thaw cycles that can significantly reduce viral titer and infectivity. It is advisable to mix the lentivirus aliquots with a cryoprotectant, such as sterile glycerol or DMSO, to a final concentration of 5-10%. This helps protect the viral particles from damage during the freezing process.
  3. Process:Carefully label each vial with relevant information, including the date of preparation, viral titer, and any genetic modifications. Place the aliquoted vials in a controlled-rate freezing container or use a styrofoam box to achieve a gradual temperature decrease before transferring them to the liquid nitrogen storage tank.
Considerations and Best Practices
  • Safety Precautions: Given the biohazardous nature of lentiviruses, ensure that all procedures for handling, aliquoting, and storage comply with institutional biosafety regulations. Wear appropriate personal protective equipment and use biological safety cabinets.
  • Inventory Management: Maintain a detailed inventory of the stored lentiviral aliquots, including their specific locations within the liquid nitrogen storage system, to facilitate easy retrieval and avoid cross-contamination.
  • Thawing for Use: When retrieving lentivirus from liquid nitrogen storage, quickly thaw the aliquot in a 37°C water bath and immediately proceed to the intended application to maximize viral integrity and infectivity.
  • Alternative Storage Options: While liquid nitrogen offers an option for long-term storage, lentiviruses can also be stored at -80°C for several months without significant loss of infectivity, provided that freeze-thaw cycles are minimized.
Conclusion
Storing lentivirus in liquid nitrogen represents a viable strategy for preserving viral integrity and infectivity over extended periods. Adhering to meticulous preparation, safety guidelines, and proper cryopreservation techniques will ensure the lentivirus remains a potent tool in your research endeavors.
Should you require further insights into lentiviral storage practices, handling, or applications, please do not hesitate to reach out. I am here to support your research efforts and facilitate your success in the field of lentiviral vector studies.
Warm regards.
This list of protocols might help us better address the issue.
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how to covert solid quantity into liquid(microlitre)?
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1. If you distribute solid nanoparticles in water (solvent), then this process is called preparing a dispersed system, not a solution.
2. To quantitatively prepare such a dispersed system, you had to write the amount of solid phase in it.
3. To prepare 50 microliters of a 5% dispersion of silver nanoparticles in water, you must weigh 5 micrograms of nanoparticles, place them in a microtube and add 45 microliters of water using a micropipette.
4.how to cover solid quantity into liquid(microliter)?
To do this, it is necessary to place solid nanoparticles in a measuring microtube, melt them and determine the volume.
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I need an oil that is inert to Gallium
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Try paraffine (= pure alkane) oil; they should be inert to gallium up to 200 °C, provided that no oxygen is present.
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If liquid crystals represent a bridge from the solid state of matter to the liquid state. Is there a bridge between the liquid state and the gaseous state of matter?
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A liquid crystal is not exactly a bridge between solid and liquid states.
Actually, there is an ambiguity defining the two states. If one defines a solid by crystalline long range order (static, structure), liquid crystals are on the solid side. Instead, if one makes the distinction by viscosity or a (relatively arbitrary) relaxation time (dynamics), liquid cystals are liquid. Conversely, glasses are liquid or solid, repectively.
The common distinction between solid and liquid states is the dynamic definition. Then, glasses are "solid" and liquid crystals are ... "liquid"!
In theory, instead, glasses are studied within the formalism of liquid theory because presence or absence of periodicity induces important differences.
Beyond a critical point, a fluid shares properties of liquids (e.g. high density) and gases (absence of free surface).
There are other common intermediate states, like gels, emulsions, plymer melts, ...
Every classification is partly arbitrary...
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One of my student is working on prediction of vapor liquid equilibria of CO2-water-MEA system using electrolyte NRTL model. She feels difficulty in calculating activity coefficient of the components; CO2, water, and MEA. Any sample calculation will be very helpful.
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She can use ASPEN Plus or DWSIM to get the activity coefficient at different temperature and composition.
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I usually use PEG-200 and PEG-300 that are in liquid form.
Recently I received PEG-100 from a company that usually do not make PEG-100, and made it once specially for us.
It is not in liquid state, or granules or flakes form, but it is one big solid that looks like in the picture attached.
I tried to melt it up to 100 C, but it did not melt.
How should I use it? My purpose is to use it as a plasticizer for aqueous tape casting, and to mix it with powder, binder and water.
Thank you.
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Another crucial information may be supplied by the company concerning the type/name of the initiator used in the addition ROP of ethylene oxide. This is important in that end groups usually have their own intrusion in many properties and behaviors. Also you may ask if the PEG 100 has been the subject of further post- polymerization treatment. Best of luck in your work.
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.nonionic liquid
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Hey there Ghazal Tuhmaz!
Alright, let's dive into why we're all about using nonionic liquids in the wire explosion plasma method for creating metal nanoparticles.
First off, nonionic liquids bring some serious perks to the table. They're like the cool, calm, and collected sidekick in this explosive process. Unlike their ionic counterparts, nonionic liquids don't carry an electric charge. This means they play nice with metals during the explosion phase without causing any unwanted reactions or disruptions.
Now, onto the wire explosion plasma method. Picture this: we're zapping a thin metal wire with a super high-voltage pulse of electricity. This sends shockwaves through the wire, causing it to literally explode into tiny droplets.
Here's where the nonionic liquid swoops in like a superhero. It acts as a stabilizer, surrounding those newly formed metal droplets and preventing them from clumping together like unruly magnets. This helps us maintain control over the size and distribution of our precious metal nanoparticles.
In essence, nonionic liquids are the unsung heroes of the wire explosion plasma method. They keep the chaos in check and ensure we walk away with beautifully dispersed metal nanoparticles ready to work their magic in various applications.
Hope that sheds some light on why we're all aboard the nonionic liquid train for this explosive endeavor! If you've got more questions or need further elaboration, don't hesitate to give me a shout. Cheers Ghazal Tuhmaz!
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Is pressure directly proportional to area in fluids and speed of liquid increase and its pressure decrease when a liquid passes through the construction in a horizontal pipe?
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In fluid mechanics according to continuity equation A1 X V1 =A2 X V2 , showing velocity inversely proportional to cross sectional area , since in FM it is well known that velocity is inversely proportional to pressure, this inverse relation of velocity with area and pressure gives direct relation. As area decreases velocity increases and pressure at that point decreases. But we also know that pressure is inversely proportional to area[P=F/A]. The higher the velocity of a fluid (liquid or gas), the lower the pressure it exerts. This is called Bernoulli's Principle. Fluid pressure is caused by the random motion of the fluid molecules. Pressure equals force divided by area ( P = F A ). The equation shows that pressure is directly proportional to force, but inversely proportional to area. At a constant area, pressure increases as the magnitude of the force applied also increases. Similarly, in fluid flow, when the fluid speeds up (increases its velocity), it has less energy to exert as pressure. So, as the speed of the fluid goes up, the pressure it can exert goes down, and vice versa. As per equation of continuity, when the liquid flows through a constriction, the area of cross-section of the liquid decreases, therefore the velocity of the liquid increases. Thus, as the fluid passes through the constriction or throat, the higher speed results in lower pressure at the throat.
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Why does velocity increase when area decreases and why does the speed of a liquid increases and its pressure decreases?
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The principle of continuity, the principle of conservation of mass. These two principles are an important reason and an important explanation behind the fact that, when the area of flow is decreased, the velocity of flow has to increase in the same proportion for the two principles to remain valid. Incompressible fluids have to speed up when they reach a narrow constricted section in order to maintain a constant volume flow rate. This is why a narrow nozzle on a hose causes water to speed up. Since velocity is high at the narrow constriction, hence the pressure is low there. For one thing, the velocity of fluid is related to the resistance of the fluid in contact with the tube. As the cross-sectional area increases, so does the surface area of the inside of the tube, resulting in a greater resistance to flow. If the area decreases the pressure increases as given by P = F/A. But, according to Bernoulli's Principle, as the area decreases, the speed increases, and as the speed increases, the pressure decreases. The pressure on a surface is inversely proportional to the area of the surface, provided that the force applied on it remains constant. Therefore, the pressure will decrease if the area on which it is applied is increased. Additionally Flow rate and velocity are related by the equation Q = Av where A is the cross-sectional area of flow and v is its average velocity.
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ln[Yl(1+cos theta/2)^2] = -2beta(Ys -Yl)^2 +ln(Ys) A plot of the left side of the equation against the liquid interfacial energy gives parabolic curve, and second order fitting is done.
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Sorry I cannot help
G. Bognolo
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I need the procedure to find the surface energy of a solid substrate. I know only the contact angle ?
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Sorry I cannot help.
G. Bognolo
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For example, If I have lactic acid and glycerol how can I know the visual form?
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thanks so much!!
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Nonionic
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Ghazal Tuhmaz Nonionic liquids are used in the wire explosion plasma method for metal nanoparticles due to their high thermal stability, anti-aggregation properties, tunability, and potential environmental benefits.
  • Malekzad, Hedieh, Parham Sahandi Zangabad, Hamed Mirshekari, Mahdi Karimi, and Michael R. Hamblin. "Noble metal nanoparticles in biosensors: recent studies and applications." Nanotechnology reviews 6, no. 3 (2017): 301-329.
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To be developed.
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Good Question
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What happens to the molecules of a liquid when you decrease its temperature and what happens to the molecules of an object as its temperature changes?
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Dr Joel Junior thank you for your contribution to the discussion
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I would like to explain my question with the following illustrative situation. In general, when we apply the high-pressure and high temperature to the solid materials, the solid melts and it goes into the liquid state. What will be nature of the structure of the liquid state?
In precise, I want to give one example. I take some solid materials like BiSe, Bi2Se3, Bi2Se5, Bi3Se7, Bi4Se10e tc. In all these compounds, the basic elemental unit is two, i.e., Bi and Se, but the composition is different.
If I apply high-pressure and high temperature, all these solid materials melt and goes into liquid state.
Does all liquid’s structure and nature same irrespective of the starting composition? [Or] Is it depends on the initial composition? Please let me know.
Your valuable explanation, suggestion, and guidance will be very useful to our research works. Thank you very much in advance.
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Thank you Ulrich Deiters for your answer and valuable time.
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Why does the speed of a liquid increase and its pressure decrease and why does velocity decrease when cross-sectional area increases?
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Dr Murtadha Shukur thank you for your contribution to the discussion
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What happens to the molecules of liquid water when changing in gaseous water and what is the weakest force of attraction between molecules?
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Dr Murtadha Shukur thank you for your contribution to the discussion
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Hello everyone,
It is known to us that vacancy exists in the solid phases, which contributes to the diffusion mechanisms.
My question is, does vacancy exist in a liquid phase? If it exists, does it affect diffusion mechanisms? Alternatively speaking, what is the diffusion scenario in the liquid phase?
It should be noted that this discussion is aimed at the metallic systems, or at least inorganic systems.
Looking forward to any answer....
Thanks!
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thanks for your kind answer
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Why does pressure increase when area increases and speed of liquid increase and pressure decrease when the liquid passes through a narrow part of a pipe?
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Your observations are partially correct. Here's a breakdown:
  1. Pressure and Area: You're right that pressure generally increases with a decrease in area. This is because pressure is defined as force per unit area. Imagine pushing a constant force on a piston. If the piston has a smaller area, the force gets concentrated on a smaller surface, resulting in higher pressure. This principle applies to fluids as well.
  2. Pressure and Speed: However, pressure and speed don't always have a straightforward relationship. In incompressible fluids (like water), there's a principle called Bernoulli's equation that governs the relationship between pressure, speed, and height. This equation states that in a steady flow, the total mechanical energy (including pressure, kinetic energy due to speed, and potential energy due to height) of a fluid element remains constant along a streamline. So, if the speed of the liquid increases, its pressure must decrease to maintain the same total energy. This is why the water pressure drops as it flows through a narrower section of a pipe.
  3. Narrower Section and Pressure: In a horizontal pipe, when the area decreases, the liquid needs to speed up (as per the continuity equation) to maintain the same flow rate. This increase in speed, according to Bernoulli's equation, leads to a decrease in pressure. So, your observation about pressure decreasing in a narrow pipe is correct.
Therefore, the relationship between pressure, area, and speed depends on the specific context. Remember that Bernoulli's equation applies to incompressible fluids, steady flow, and a single streamline. In more complex situations, other factors like friction and turbulence can come into play.
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Can a solid turn into a gas without becoming a liquid and what happens to the average velocity of a gas if the temperature increases?
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The process of sublimation causes solid to vapor transition without going through the liquid phase. Examples include naphthalene, camphor, etc.
Increase in temperature adds to the KE of the gas molecules resulting in greater velocity.
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I am making electrodeposition liquid of tin antimony, and I want to dissolve 0.3M SnCl2·H2O and 0.03M SbCl3 in 0.3M citric acid aqueous solution, but it cannot be dissolved and white precipitate appears. What is the reason?
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Xun Wang Dissolving tin and antimony in citric acid solutions is challenging due to their specific chemical interactions. Tin dissolves, forming a passivating film, while white precipitates may occur due to SbCl₃'s limited solubility or insoluble antimony compounds. Precipitation can occur due to pH, concentration, complex formation, and temperature.
  • Giannetti, B. F., Sumodjo, P. T. A., & Rabockai, T. (1990). Electrochemical studies with tin electrodes in citric acid solutions. Journal of applied electrochemistry, 20, 672-676.
  • Evans, B. S. (1932). A rapid method of dissolving lead alloys preparatory to the determination of tin and antimony. Analyst, 57(678), 554-559.
  • Zohdy, K. M., El-Sherif, R. M., & El-Shamy, A. M. (2021). Corrosion and passivation behaviors of tin in aqueous solutions of different pH. Journal of Bio-and Tribo-Corrosion, 7, 1-7.
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Can anyone recommend a company or institution (write contact) that would be able to measure supercritical conditions for a liquid mixture?
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Could you be more specific with repect to the mixture? Does “supercritical” refer to vapour–liquid phase separation? Then you would probably look for a lab that can handle high pressures. Are you interested in cryogenic systems or in phase equilibria at high temperatures?
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Hello! I need to snap freeze cancer pellets to submit for multiomics. Does anyone know of 15 mL conical tubes or Eppendorf/microcentrifuge tubes that can withstand liquid nitrogen and dry ice?
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You could test a couple of different manufacturers' vials to see which ones cope with the stress of freezing. I experienced the opposite issue with heating polypropylene vials that had been gamma irradiated in the factory and found they melted at a lower than expected temperature. Try out a few vials and see if they crack or split when frozen (or the lids fail upon returning to room temperature). You may need to adjust sample volumes or pre-cool the sample before going into liquid nitrogen.
Best of luck.
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Can a solid become a gas without becoming a liquid first and what happens to the average speed of molecules in a gas when its temperature increases or decreases?
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Dr Murtadha Shukur thank you for your contribution to the discussion
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Hello ..!
I am trying to make resol as carbon source . I am following the attached paper in this paper they mentioned to evaporate water at 50 degree in vacuum . My question is that how to know that water is evapurated. What will be the final physical state of this resol solid or liquid.
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Hello
The vacuum causes the water to boil at a lower temperature and evaporate
The connections in the vacuum cause no water to escape from the reaction vessel, so the water will remain as it is
During the reaction, you should see steam in the glass container
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I have done the NLC formulation using ultrasonication and microemulsion techniques. Now I have the whole emulsion as a milky white liquid. How to separate the NLC from the aqueous solution. I have done a single centrifugation. I assumed that the pellet had the NLC and the supernatant had the free drug. And I have to spin the emulsion at 25,000 rpm for 10 minutes. But the pellet hasn't completely settled. So please suggest a protocol to separate the NLC from the aqueous phase
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Microemulsions are thermodnamically stable, so if you want to break one you have to use pressure or temperature. Have you tried to heth or cool your emulsion? Mind you, when the emulsion breaks you loose the identity of the particle. If you want to just separate the nanoparticles and preserving their identity gravity separation is the only way. I seem to understand that you have already used ultracentifugation with some success. The other possibility is to build agglomerates of larger particles that should settle more quickly. If your system can tolerate you may try low concentrations of di or trivalent cations. Start with say 0.001 molar CaCl2 or AlCl3. If nothing happens go to higher concentraations,
Best regards
G Bognolo
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Which state of matter has the most energy and how does temperature affect the transmission speed of particles within solids or liquids?
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In addition to the right previous answer, I guess that plasma has the most energetic states since the velocity of charge particle and the radiation field is enormous, following by nuclear elements capable to be used for fusion and fission, Prof. Rk Naresh
Best Regards
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Im planning to do some BAC maxi preps, a total of 8, and due to the restrictions in terms of equipment, i would only be able to do 2 at a time since only two 1000mL Erlenmeyers fit in the shaking incubator.
I was wondering if i could do all of the starter cultures together and then leave some at 4ºC to then do the maxi cultures the following days (so the maximum time a culture would be at 4ºC would be around 3 days).
My concern is that by putting them at 4ºC ill be losing the inherent efficiency of a starter culture, i.e., to have actively dividing bacteria, in the logarithmic phase.
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My take is that starter culture preserved at 4oC for up to 24 hours will not have any significant population change. This is due to inactivation of microbial enzymes. However, if left for longer than this period, cell start entering a decline phase and therefore a lower population in the starter culture.
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Can we calculate Entrapment efficacy of liquid formulation of nanoparticles ?
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Of course Shubham Karpe, we can discuss the effectiveness of entrapping drugs within nanoparticles. The efficacy of entrapment is a crucial parameter in drug delivery systems, particularly when dealing with nanoparticles. It refers to the percentage of drug molecules successfully encapsulated or entrapped within the nanoparticles compared to the total amount of drug used during formulation. To determine the entrapment efficacy, we measure the total drug content before and after encapsulation, and calculate the difference between these amounts. This value is then expressed as a percentage by dividing it by the initial total drug amount and multiplying it by 100. The formula for calculating entrapment efficacy is:
Entrapment Efficacy (%) = [(Amount of Drug Entrapped / Total Amount of Drug Used) x 100]. It is essential to employ precise measurement techniques and reproducibility to obtain reliable results. Additionally, factors such as nanoparticle size, surface charge, and composition can significantly impact entrapment efficacy, and should be carefully optimized during formulation to achieve the highest entrapment efficacy.
One interested reading:
Please let me know if you Shubham Karpe require further clarification or assistance with your calculations.
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What happens when thermal energy is added or removed from a liquid and what happens to temperature as thermal energy is removed?
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Dr Murtadha Shukur thank you for your contribution to the discussion.
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How is thermal energy related to entropy and what happens when thermal energy is added to liquid?
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Thermal Energy, Entropy, and Liquids
Thermal energy and entropy are closely linked, especially when considering liquids. Here's how:
Thermal Energy:
  • Represents the internal energy of a system due to the random motion of its atoms and molecules.
  • Higher temperature signifies higher thermal energy due to faster molecular movement.
Entropy:
  • Quantifies the "disorder" or randomness within a system.
  • In simple terms, entropy increases as things become more spread out and disorganized.
  • It's not directly related to the total amount of energy, but rather the availability of that energy for doing useful work.
Adding Thermal Energy to Liquids:
  1. Temperature Increase: When you add thermal energy to a liquid, its molecules move faster, raising its overall temperature.
  2. Entropy Change: This increased molecular motion translates to increased entropy. Imagine ice cubes melting in water; the ordered structure of ice (low entropy) transforms into the more dispersed water molecules (higher entropy).
  3. Energy Availability: However, not all the added thermal energy remains "usable." Some is "lost" as the temperature becomes uniform throughout the liquid, increasing entropy and decreasing the potential for doing work. This is why, for example, a hot cup of coffee eventually cools down and loses its ability to make your hands warm.
Here are some additional points:
  • The Second Law of Thermodynamics states that the total entropy of an isolated system always increases over time. This means systems naturally tend towards higher disorder, making processes like ice melting irreversible.
  • External work can temporarily decrease entropy in a specific area, but overall entropy in the surrounding system still increases. For example, a refrigerator uses energy to remove heat from its interior, but this creates waste heat outside, increasing the overall entropy.
Summary: Adding thermal energy to a liquid increases its temperature and entropy. However, some of this energy becomes "unusable" due to increased randomness, highlighting the connection between thermal energy and the concept of entropy.
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Small intestinal pressure is one of the GI motility indicators and its manometry is very advanced nowadays. I wonder if there is any liquid inside the lumen during manometry. I already know the patient must be fasted for hours before the examination. So, I guess the lumen is filled with air and what is measured is the air pressure instead of liquid pressure. Is there any mistake?
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There is often liquid in the lumen during an Esophageal manometry that does cause pressure changes, called the Intra-Bolus pressure. The impedance technology can tell us if it's liquid or air.
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Every time I conduct protein crystallization experiments, it takes a considerable amount of time, often extending into the late hours of the night. Therefore, I am considering flash-freezing the purified protein samples in liquid nitrogen and storing them in a -80°C freezer. The idea is to thaw them the next day and continue the experiments. Is this approach viable?
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The critical point in freezing proteins for storage are the freezing and thawing processes. While freezing is a good solution for long-time storage, I would avoid repeatedly freezing and thawing the protein just to avoid keeping it for a few hours at 4° C. For the same reason, if you need the same protein prep for multiple experiments, it is better to aliquot the stock solution before freezing it, rather than repeatedly thawing the stock just to take out a small fraction, then freezing it again.
Of course, it very much depends on the specific protein and other components of the sample how much damage can be done by either storage at 4° or by freezing/thawing.
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The operator of FTIR spectrometer says because of water and OH peak, you won't have a good result.
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FTR ATR’s can be quite useful in such studies of dry material (pure material). Wet samples will indicate the water peaks. If water is the only thing to evolve, dry can produce a very crisp spectrum.
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I am a final year Masters's Student from Heriot-Watt University currently working on my dissertation project titled "A THEORETICAL ASSESSMENT OF THE STRUCTURE OF A LIQUID STORAGE TANK UNDER SEISMIC FORCES" with the following objectives:
1. Verification of Current Theories (Housner, Preethi, and Malhotra) of liquid Structure Behavior (sloshing wave height) under seismic forces for petroleum-filled storage tanks using Finite Element Modelling and Finite Element Analysis.
2. Assessment of the possible failure mechanism of the superstructure of the various liquid storage vessels under exposure to seismic forces using Finite Element Modelling and Finite Element Analysis based on the API 650 Design Standard.
3. Proposal and initial assessment of the effectiveness of a Bass Isolation System on the sloshing wave height using Finite Element Modelling and Finite Element Analysis.
Can the Ansys modal analysis module be used to model a fluid-filled storage tank and determine the sloshing wave height along with the impulsive and convective mass components of the fluid based on the application of specific Acceleration, Velocity, and displacement values?
Can I subsequently transfer the model to the Ansys Static Structural Module to determine the various resulting stresses that will develop within the tank structure due to the seismic forces and the fluid-structure interactions?
If not, can you guys offer any advice on what methodology I should take?
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Congratulations to you! Could you please share your thesis?
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Dear all,
I would like to know if anyone has experience with doing MD simulation of nucleic acids or proteins in high density liquids.
I would appreciate if I could be guided to some reading material
The idea is to restrain the movement of Dna so that the effect of uv damage can be studied
Thank you
Ayesha
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Dear Soykan,
Thank you for the answer.
Could you please share the details of the setup if that is OK with you.
Thank you
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Low cost approach to solve issue raised due to erosion -corrosion in pump with liquid Aqueous urea. Its a diaphagram pump. Researching in this area. what areas i should focus for this .
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Manchimsetty Srinidhi Material Science/Materials are finding innovative solutions to problems for many equipment, including pumps, you would want to find options and based on your business needs (sales volume, criticality of quality,...) redesign the pump using new materials, a lot of people are using composites, needs capital investment, but pays off for many. 3D printed components may another option! Best of luck with your very interesting project.
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It's Working
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Stabilizers interfere with interactions between molecules in the solution, preventing them from clumping together or separating and Some stabilizers carry electric charges that repel each other, creating a barrier between particles. This electrostatic repulsion prevents aggregation, keeping the solution dispersed.
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Is it okay to use liquid argon as an inert gas in a glove box?
I'm currently using argon gas, and it needs frequent replacements, which is inconvenient. I'm wondering if using liquid argon might extend its usability period and safety
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A consideration when using liquid argon is that it boils at -185.7 Celsius whereas oxygen boils at -183.0 Celsius, so oxygen from the atmosphere can condense on the argon and contaminate it, thereby, ruining an oxygen-free atmosphere. However, argon (40 amu) is also slightly heavier than diatomic oxygen (32 amu) and, therefore denser, so the contamination would only occur if air were blown over the surface of the liquid argon.
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I have worked on liquid liquid phase separation and single particle tracking during cold shock response. I have used DeaD PAmCherry and treat it with 1,6 hexandiol and collected data in the form of fluorescence imaging and bright field and do data analysis by MATLAB. I draw D-histogram of different reviews. What the new things I will add in my project?
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Outside my field. sorry
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How can I derive polynomial functions that can predict the viscosities of pure liquid water in function of temperature at atmospheric pressure? I used experimental data from literature, but the equation that I derived by using Excel but after derived the equation when I putted the value of temperature (independent variable), it's not worked!
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Please see eq. 7 of J. Pátek, J. Hrubý, J. Klomfar, M. Součková, and A. H. Harvey, J. Phys. Chem. Ref. Data 38, 21, 2009. This is the result of a careful and critical examination of the literature data.
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Ionic liquids synthesis and purification is important task in chemical laboratories because of the liquid phases.
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Thank you for sharing@Abdelhak Maghchiche
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This question is an extension and an attempt join the following papers:
It also aims to advance a decade long effort to formulate and articulate a new theory of learning as initiated by this visual essay/ research report:
Commonalities Between Aristotle's Eudaimonia & Zhuangzi´s Dao
Eudaimonia in ancient Greek philosophy and the Dao (Tao) in Chinese philosophy, particularly in Daoism (Taoism), share some similarities in their overarching themes and perspectives on living a meaningful and fulfilling life.
However, they arise from distinct cultural, philosophical, and historical contexts. Here are some comparisons between Eudaimonia and the Dao:
Harmony with Nature:
Eudaimonia: In Greek philosophy, living in harmony with nature is associated with the idea of aligning one's life with the order of the cosmos and with reason. It involves recognizing and fulfilling one's potential as a rational being.
Dao: Daoism emphasizes harmony with the Dao, which is often described as the fundamental principle or force that underlies and unifies the universe. Living in accordance with the Dao involves attuning oneself to the natural order and rhythms of existence.
Virtue and Conduct:
Eudaimonia: Virtue is a central component of Eudaimonia, and philosophers like Aristotle identified specific virtues (e.g., courage, wisdom, justice) as essential for a flourishing life.
Dao: Daoism also values virtues, but it often emphasizes a more spontaneous and natural expression of virtue. Virtuous conduct is seen as flowing from being in harmony with the Dao rather than being rigidly prescribed.
Non-Striving and Wu Wei:
Eudaimonia: While Greek philosophy emphasizes the importance of virtuous action and the development of one's potential, it doesn't explicitly advocate a concept similar to Daoism's wu wei (non-action or non-striving).
Dao: Wu wei is a central concept in Daoism, suggesting that one should act in accordance with the natural flow of the Dao, without unnecessary effort or force. It involves spontaneous and effortless action, aligning with the Dao's inherent order.
Individual vs. Cosmic Perspective:
Eudaimonia: The focus in Greek philosophy is often on individual flourishing and the development of personal virtues within the context of the polis (city-state) and social life.
Dao: Daoism often takes a more cosmic and holistic perspective, emphasizing the interconnectedness of all things. The Dao is not just a personal path to follow but a universal principle that permeates everything.
Ethics and Morality:
Eudaimonia: Greek philosophy, particularly in the ethical teachings of philosophers like Aristotle, provides a systematic framework for moral reasoning and the cultivation of virtues.
Dao: Daoism, while valuing moral conduct, tends to approach ethics in a more intuitive and context-dependent manner, with an emphasis on spontaneity and responsiveness to the situation.
The Holobiont through the Worldviews of Aristotle & Zhuangzi
The holobiont is a biological concept that refers to a host organism and its associated community of symbiotic microorganisms, collectively known as the microbiota. This concept challenges the traditional view of an organism as an individual and emphasizes the interconnectedness between a host and its microbial inhabitants. Let's explore how the holobiont concept might relate to the synthesis of Eudaimonia and Dao:
  1. Interconnectedness and Harmony:The holobiont emphasizes the interdependence of the host organism and its microbiota. Similarly, in the synthesis of Eudaimonia and Dao, there can be an emphasis on interconnectedness and harmony, recognizing the symbiotic relationship between the individual's virtues and the broader cosmic order.
  2. Microbial Diversity and Virtuous Living:Just as a diverse microbiota contributes to the health of the holobiont, a diversity of virtues and perspectives might contribute to the well-being of an individual pursuing Eudaimonia. The synthesis could involve embracing a variety of virtues and ethical principles to create a more resilient and balanced life.
  3. Adaptability and Wu Wei:The holobiont must adapt to changes in its environment, and the Daoist concept of Wu Wei involves acting in harmony with the natural flow. The synthesis could encourage adaptability and the ability to navigate life's challenges with a sense of flow and spontaneity.
  4. Holistic Well-Being:The health of the holobiont is not solely determined by the host organism but by the balance and well-being of the entire community. In the synthesis, holistic well-being could involve not only individual virtues but also the well-being of the broader community and environment.
  5. Balance of Individual and Collective:The holobiont concept challenges the notion of an organism as an isolated individual. Similarly, the synthesis could challenge an overly individualistic approach to Eudaimonia by recognizing the importance of the collective and the interconnectedness of individuals within a larger societal and cosmic context.
  6. Microbial Influence on Behavior:The microbiota can influence the behavior and health of the host organism. In the synthesis, there might be an acknowledgment that external factors, including cultural and environmental influences, play a role in shaping individual virtues and ethical choices.
  7. Cultivation of Inner and Outer Ecosystems:The holobiont concept encourages consideration of both the inner and outer ecosystems. Similarly, the synthesis could involve the cultivation of inner virtues (Eudaimonia) and an awareness of one's place in the larger cosmic and societal ecosystem (Dao).
  8. Dynamic Equilibrium:The holobiont exists in a dynamic equilibrium with its microbiota. In the synthesis, there could be an appreciation for the dynamic nature of virtue ethics and the need for ongoing self-reflection and adaptation in the pursuit of well-being.
In essence, the holobiont concept provides a biological metaphor that can be extended to philosophical and ethical considerations. It encourages a more interconnected and holistic perspective, aligning well with the synthesis of Eudaimonia and Dao that seeks to integrate individual virtues with a broader understanding of cosmic harmony and balance.
The Liquid (Holobiont) Learner in a Holistic Learning Theory: The Symbiotic Path to Flourishing Wisdom
To integrate insights from Eudaimonia, Dao, and the holobiont concept to propose a holistic theory of learning that goes beyond traditional educational paradigms. This theory posits that learning is not merely an individual cognitive process but a symbiotic relationship between the learner, their virtues, the cosmic order (Dao), and the broader learning ecosystem.
  1. Eudaimonic Virtue Learning: Learning is seen as the cultivation of virtues that contribute to individual flourishing. Just as the holobiont thrives on microbial diversity, learners flourish by embracing a diversity of virtues—wisdom, courage, compassion, and resilience. The goal is not just knowledge acquisition but the development of a virtuous character.
  2. Daoist Harmony in Learning: The Daoist concept of Wu Wei, or effortless action, is applied to the learning process. Learners are encouraged to align with the natural flow of curiosity, exploration, and understanding. Learning becomes a harmonious dance with the cosmic order, emphasizing spontaneity, adaptability, and a balance between active pursuit and receptive contemplation.
  3. Interconnected Learning Ecosystem: Borrowing from the holobiont concept, the Liquid (Holobiont) Learner theory recognises that learning is a collaborative endeavor involving not only the individual learner but also the learning environment, peers, mentors, and cultural influences. Just as the holobiont exists in dynamic equilibrium, learning thrives when there is a balanced exchange of ideas, perspectives, and experiences within the learning ecosystem.
  4. Cultivation of Inner and Outer Knowledge: Similar to the Daoist emphasis on inner virtues, the Liquid (Holobiont) theory encourages the cultivation of inner knowledge—self-awareness, emotional intelligence, and ethical understanding. This inner wisdom complements the acquisition of external knowledge, fostering a well-rounded and balanced approach to learning.
  5. Adaptive Learning Resilience: Acknowledging the dynamic nature of both the Holobiont and Dao, the Liquid Learner theory promotes adaptive learning resilience. Learners are encouraged to adapt to changing circumstances, embrace the unexpected, and find opportunities for growth in challenges. The learning journey is viewed as a continual process of adaptation and evolution.
  6. Holistic Well-Being in Learning: Holistic well-being is a central goal of our learning theory. Beyond academic achievement, the theory emphasizes the well-being of the learner as an individual and their contribution to the well-being of the learning community. Learning becomes a transformative journey that enhances not only cognitive abilities but also emotional, ethical, and social dimensions of well-being.
Thus the Liquid (Holobiont) Learner theory envisions a symbiotic relationship between the learner, virtues, cosmic harmony, and the learning ecosystem. It embraces diversity, spontaneity, and adaptability, fostering not just the accumulation of knowledge but the development of virtuous individuals who contribute to the flourishing of the broader learning community and the cosmic order.
Please see also:
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I am also interested how TECHNOLOGICAL TURBULENCE is connected to HUMAN AND MACHINE REDUNDANCIES
Please any overlapping questions.
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I used the chemical compound dimedone, which was purchased 13 years ago, as a starting material for the synthesis of other compounds, but unfortunately, it was contaminated with two other materials. The newly synthesized materials stayed in a liquid state and didn't solidify. Does the age of the starting material affect the physical status of the newly synthesized compounds?
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It is better NOT to use it. Organic compounds can degrade over time, leading to changes in their chemical properties and potential loss of effectiveness. Here are some pros and cons of using expired organic compounds:
Pros of using expired compounds:
1. Cost-saving: Expired compounds may be available at a lower cost or even free of charge, making them attractive for researchers or industries operating on a tight budget.
2. Limited applications: In some cases, expired compounds may still be suitable for certain applications where their degraded properties do not significantly impact the desired outcome. For example, if the compound is being used as a reference standard or for non-critical preliminary experiments.
3. Educational purposes: Expired compounds can be useful in educational settings for teaching purposes, demonstrations, or as practice materials for students to familiarize themselves with laboratory techniques.
Cons of using expired compounds:
1. Decreased potency or activity: Over time, organic compounds can undergo chemical reactions that lead to degradation, resulting in reduced potency or activity. This can affect their performance in experiments or applications, making them less reliable or ineffective.
2. Inaccurate or inconsistent results: Using expired compounds can introduce variability and uncertainty in experimental results. This can be problematic, especially in research or industrial settings where accuracy and reproducibility are crucial.
3. Safety concerns: Expired organic compounds may pose safety risks. Their degradation products or impurities could be toxic, unstable, or reactive, potentially leading to hazardous situations during handling or when used in reactions or processes.
4. Unreliable data: When using expired compounds in research or industrial processes, the obtained data may not be reliable or representative of the compound's true behavior. This can hinder scientific progress, lead to erroneous conclusions, or impact product quality in industrial applications.
5. Regulatory compliance: In regulated industries, the use of expired compounds may not comply with quality control standards or regulatory requirements. Using expired compounds could result in non-compliance, potentially leading to legal or regulatory consequences.
Hope it helps:credit AI
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My research is green synthesis of urea nanoparticles and I prepared urea nanoparticles contain solution. I need to characterize my sample, but it is difficult to obtain sufficient amount by powder form. Can I get SEM images from liquid nano urea particles contain sample. Is there available easy method to dry my sample?
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Obtaining SEM images from liquid samples, especially those containing nanoparticles, can be challenging. Traditional SEM requires a vacuum environment, which is not compatible with liquid samples as they would evaporate or be damaged.However, there are specialized techniques like environmental SEM (ESEM) or cryo-SEM that can be employed for imaging hydrated or frozen samples. These methods allow for imaging in the presence of water or other liquids. Consider consulting with microscopy experts or utilizing facilities equipped with such instruments for your specific sample requirements.
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Calculating the specific heat of a simple liquid by the number of elastic oscillators.
Calculate the specific heat of a simple liquid using the number of elastic oscillators
Each liquid molecule has an average of 8 elastic oscillators around it, and the specific heat contributed by the elastic energy is 4R。Therefore, near the three phase points, the specific heat at constant pressure of a single atomic liquid is 5.5R, and the specific heat at constant pressure of a diatomic liquid is 6.5R. Low temperature liquids such as Ar, Kr, Xe, O2, N2, F2, etc. conform to this conclusion.
Please read the following link for details
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Thank you for your reply.
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please i would like to collect all the parameters related to the synthesis of nanoparticles using laser ablation, in terms of laser parameters, liquid parameters, environment parameters and if any other parameters
Thanks
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The key parameters that can influence the size, shape, and properties of the nanoparticles produced are: Laser Parameters, Wavelength, Pulse Duration, Pulse Energy, Target Material, Type of Material, Liquid Environment, Type of Liquid and Experimental Setup and so on…
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I want to digest sumo/His label when the protein in still hang on the Ni column. I wash the undesired proteins as usual, then balance the column with 3ml enzyme digestion buffer, then add sumo enzyme for digesting the fusion protein at 4℃ for 12h in 4ml sumo digesting buffer. Then catch the flow through liquid, take some sample from flow through liquid and beads after elution for SDS-PAGE, the brand explains the label did not have been cut, it is still on the beads entirely.
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G.Dharmamoorthy First of all, I really appreciate your help. About changing buffer. I already balance the column with sumo cleavage buffer before adding the sumo protease. Unfortunately is still not working.
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Hi everyone,
I'm new in ASPEN, my question is related to the possibility of simulating an adsorption column where on one side you have a stream of pure hydrogen and on the other side you have a stream of a liquid metal. The objective of this column is to solubilize the hydrogen in the liquid metal according to Sieverts' law. Then, as an apposite step, I would like to simulate the stripping of the hydrogen solubilized in the liquid metal by using an inert gas.
My question is twofold: 1) is it possible to add a liquid metal with known thermophysical and transport properties in ASPEN? 2) how do I have to implement the Sieverts' law since only Henry'ss law seems to be implemented in ASPEN?
The absorption column is indeed a packed column.
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Luigi Candido , Hi luigi. Any update on your findings?
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I have used PEG as supposed it would be stable in ph 2 and ph 7 and by shifting their peak can understand ho much is the retention time, but have nt yet get any good results
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Thank you for your answer, I ended up using pepsin as a protein marker which is stable in acidic pH.
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For anyone that has used a Talos S/TEM with a large liquid nitrogen dewar attached, how do you fill your tank? The inside of the microscope is very cramped, and it is awkward to pour the liquid nitrogen from a transfer vessel into the dewar. We have been looking at purchasing a 10-20 L dewar fitted with a withdrawal device and hose attachment so we can transfer the liquid nitrogen directly from a large dewar into the microscope's dewar. I'm curious if there are any other solutions I'm overlooking.
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If this helps anyone, here was the solution we came up with.
We ordered the following parts from a liquid nitrogen supply vendor:
  • 20 L Al container for storing liquid nitrogen (LN2)
  • Siphon head for transferring liquid nitrogen (LN2 transfer valve, vent valve, manometer, 0.5 bar overpressure value, clamp, O-ring with centering ring, screw adapter)
  • Roller base
  • LN2 transfer hose with PTFE piping and phase separator
Once the 20 L container is out of LN2, we vent it, take off the siphon head, insert a transfer hose from a large LN2 container, and fill our 20 L container nearly to the top. We let the metal at the opening warm a bit and then attach the siphon head. We move it into our TEM lab so that it can build up pressure overnight. Then, we simply insert the transfer hose on our container into the dewar on the microscope, and the pressure pushes it up the hose. We only have to do this refilling process about once a week.
Here is a video showing the overall procedure: https://youtu.be/zrCT433gaK0?si=0jMnaafOOavsEepc
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If I add a dye to a liquid, and this liquid is subjected to a high temperature may reach 110 oC does this temperature will affect the dye colour or not
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High temperatures, especially around 110°C, can affect the color of dyes. The extent of the effect depends on the specific dye's type and chemical properties.
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When I conduce an esterification reaction using oleic acid P.A, occurs a variation in color of final product generated (liquid). Is this color due occurence of some concurrent reactions generating other products in medium? Which are the majority products in this reaction that causes this phenomenon?
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Dear Arthur,
A color change is possible. In your case, you use oleic acid to carry out an esterification therefore your product may have a totally different physico-chemical behavior explaining a reduction in oleic acid and therefore in your initial coloring of your reaction medium.
Do you use pure products and not a heterogeneous mixture like an oil (oilive oil for example)? But even in the case of an vegetable oil, you will see different behavior during the reaction.
best regards
Benoît
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All the methods to synthesize silver graphene quantum dots that i can find are in liquid form. I do not have the facility of testing liquid samples for FTIR, XRD, SEM etc as the testing centre requires solid or dry samples only.
Can anyone please suggest me if i can get the nanocomposite by centrifuging the liquid samples? Or any other method entirely to synthesize the nanocomposite in the solid form?
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Lyophilization of the silver doped quantum dots can be performed. First you need to freeze them using liquid nitrogen and further lyophilize it for atleast 24 hours.
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steps of preparation a semen cryoprotectant.
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Definitely, not using a diluent to preserve sperm morphology and functionality will affect its conservation possibilities. In this sense, a number of cryoprotective media and cryopreservation methods have been developed, that have given different results in the preservation of sperm from various species. The variety is such, and the sperm behavior of each species is so different, that it is impossible to design a combination cryoprotectant diluent - cryopreservation process unique for all species. Therefore, first of all, to help you, we must know what species you are referring to, and accordingly send you to the appropriate bibliography for the case, which, in general, is extensive for slaughter animals.
Hope it helps.
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We have liquid waste from the by-product of molasses fermentation into glutamic acid. The problem is how to concentrate on this waste. If you have a suitable membrane suggestion, please let me know.
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Please Ref:
Study on molasses concentration ....... N. idris....2018
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I will use hydroiodic acid to wet etch tin dioxide, but how to treat hydroiodic acid waste liquid is a problem.
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While stirring the waste material containing Hydroiodic acid , adds large amounts of an ice water solution ( 1:10) of base in such as sodium bicarbonate, calcium hydroxide, or sodium hydroxide for neutralizing concentrated acid . Dispose of solution down the drain followed by 20 parts water to the neutralized solution.
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J.R. Grace, T. Wairegi, T.H. Nguyen, Shapes and velocities of single drops and bubbles moving freely through immiscible liquids, Trans. IChemE. 54 (3) (1976) 167–173.
Grace, J.R. (1973) Shapes and Velocities of Bubbles Rising in Infinite Liquids. Transactions of the Institution of Chemical Engineers, 51, 116-120.
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I'm afraid professor Grace has since then passed and the above link no longer works. I would also like to access his paper from 1976, does anyone have the paper/know of a way to gain access to a copy? Kind regards
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Solute is toxic liquid so i have to use in microliter.
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Peter A. M. Steeneken It is a good point to clarify what kind of ppm is desired. In my "bubble" ppm means, when nothing else is specified, ppm with respect to amount of substance, weight and volume would be specified by ppmw or ppmv.
Therefore, Rehana Zia, check first which ppm you need and follow my Link or Dr. Steeneken's instructions, accordingly.
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Which part of the Earth receives the least sun's rays and what part of the water cycle is when liquid water moves through the soil to become groundwater?
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Dr Bryan Booth thank you for your contribution to the discussion
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Which shape of water surface will give the highest rate of evaporation and why does water Vapour takes up more space than the same amount of liquid?
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The shape of the water surface that will give the highest rate of evaporation is a convex surface. This is because the depth of the water is lower at the edges of the surface, which allows more water molecules to escape into the air. Water evaporates faster if the temperature is higher, the air is dry, and if there's wind. The same is true outside in the natural environment. Evaporation rates are generally higher in hot, dry and windy climates. For convex water surface depth of water will be minimum, thus higher evaporation. The distilled water is evaporating the slowest, then salt water and fresh water the quickest. Evaporation increases with the increase in surface area. It is because, the larger the surface area that is exposed to air, the more molecules will escape into the air. The shape of the container, specifically the opening part where the liquid is exposed, plays a role in the rate of evaporation. If the surface area of the container is increased, a larger amount of the liquid is exposed to the air. Increase the surface area by placing the water in a shallow tray. Blow (preferably warm) air over it by creating a cross-draught or using a fan. (Warmer air holds more moisture.) Place the water in a metal container with a good thermal contact with its surroundings, so that it does not cool down as it evaporates. A large number of water vapours already present in vacant spaces of air particles restricts the entry of more water vapours. In other words, an increase in humidity decreases the rate of evaporation. Hence, evaporation will be faster in a region far away from the sea, with low humidity. Steam takes up a lot more space (it has a greater volume) than liquid water because water molecules in steam are more widely dispersed. There is a lot of empty space between the water molecules in steam and the molecules contain more energy and move more rapidly than do the molecules in liquid water. In a gas, the molecules are hardly attracted to each other at all. That's why the molecules of a gas are so far apart compared to the molecules of a liquid or a solid. In fact, water as a gas (water vapor) takes up over I, 000 times more space than the same number of water molecules as a liquid. Whereas evaporation is the transformation of liquid water to gaseous water vapor, condensation is the opposite: it is the transformation of vapor back into liquid water. When water evaporates, it expands 1600 times larger in volume to become steam. When you heat up water, the water molecules start moving around faster and faster. They bounce off each other and move farther apart. Because there's more space between the molecules, a volume of hot water has fewer molecules in it and weighs a little bit less than the same volume of cold water. Water evaporates at room temperature because the molecules at the top of the liquid have less intermolecular attraction than those within the bulk.
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When the rate of evaporation equals the rate of condensation does the partial pressure of a volatile liquid in a sealed container change?
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I agree with Murtadha Shukur, that the partial pressure of a volatile liquid in a sealed container does not change when the rate of evaporation equals the rate of condensation. This is because the partial pressure of a gas is the pressure exerted by that gas in a mixture of gases. Dynamic equilibrium is important because it helps us understand how chemical reactions work. For example, when water evaporates from a glass, it eventually reaches dynamic equilibrium where the rate of evaporation equals the rate of condensation. This keeps the amount of water in the air constant. It reaches a stage where the rate of evaporation is equal to the rate of condensation. This phase is called the stage of equilibrium. As represented by the manometer, at this point the pressure exerted by the molecules is called the vapour pressure of the liquid. When the evaporation rate and condensation rate are equal, a state of saturation or equilibrium exists. If the air parcel is heated, the evaporation rate increases because the more energetic molecules can evaporate more easily. When the rate of condensation of the gas becomes equal to the rate of evaporation of the liquid or solid, the amount of gas, liquid and/or solid no longer changes. The gas in the container is in equilibrium with the liquid or solid. Condensation is the process by which water vapor in the air is changed into liquid water; it's the opposite of evaporation. Condensation is crucial to the water cycle because it is responsible for the formation of clouds. At any temperature, evaporation and condensation are actually occurring at the same time. Faster molecules from the liquid evaporate while slower molecules from the gas condense. Depending on the conditions, one process will happen at a faster rate than the other resulting in net evaporation or net condensation.
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Why do liquids with fewer polar bonds evaporate faster and rate of evaporation of a liquid related to the intermolecular forces acting on it?
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The more-polar molecules will stick together more and will probably evaporate more slowly than less polar molecules. Less-polar molecules should evaporate faster because they are not as attracted to each other. London dispersion forces are the weakest type of intermolecular force. Liquids with fewer polar bonds are more likely to be nonpolar. Nonpolar liquids have weaker intermolecular forces than polar liquids, so they evaporate faster. For example, acetone is a nonpolar liquid that evaporates very quickly. A liquid's vapor pressure is directly related to the intermolecular forces present between its molecules. The stronger these forces, the lower the rate of evaporation and the lower the vapor pressure. Liquids with weak intermolecular attractive forces have low heats of vaporization and are volatile they evaporate easily. Liquids with strong intermolecular attractive forces evaporate more slowly, because a greater amount of energy is needed to overcome the attractive forces between molecules. Intermolecular forces, as noted earlier, are attractive in nature. A liquid with weak intermolecular forces will evaporate quickly because it takes less kinetic energy for a molecule at the surface of the liquid to break away from the other molecules in the liquid. Evaporation occurs when energy (heat) forces the bonds that hold water molecules together to break. When you're boiling water on the stove, you're adding heat to liquid water. This added heat breaks the bonds, causing the water to shift from its liquid state to its gaseous state (water vapor), which we know as steam. The larger the intermolecular forces in a compound, the slower its evaporation rate. They all depend on the fact that some parts of polar molecules have positive charges and other parts have negative charges. The positively charged parts on one molecule align with the negative parts of other molecules. Instead, the intermolecular forces of attraction between the liquid molecules are broken or disrupted, causing the transformation into gas. These intermolecular forces of attraction are electrostatic attractions between molecules and are not inherent bonds of the molecules. When temperature increases, the heat increases resulting in higher kinetic energy of the molecules. It means that the molecules from the surface will get more energy to break the shackles of intermolecular forces and become vapour. This is the most important among the factors affecting evaporation.
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I extraction RNA from Physcomitrella patens (Moss) Following the protocol of RNAiso Plus. Firstly using a tissue lyser and added 1 ml RNAiso plus and vortex. next step Add the chloroform after centrifuge I separated the top liquid layer but the problem is yellow color liquid. I could not avoid the yellow color at ned of the extraction process after the precipitated pellet is brown.
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Hi, did u resolve this issue?
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Does the higher boiling liquid have stronger intermolecular forces than the lower boiling liquid and high viscosity mean strong intermolecular forces?
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Dr Murtadha Shukur thank you for your contribution to the discussion
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Why solubility of solid in liquid increases with increasing temperature and effect of temperature in the change from solid to liquid?
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Solubility increases with temperature for most solids dissolved in liquid water. This is because higher temperatures increase the vibration or kinetic energy of the solute molecules. Solute molecules are held together by intermolecular attractions. Solubility is the maximum amount of a substance that will dissolve in a given amount of solvent at a specific temperature. There are two direct factors that affect solubility: temperature and pressure. The solubility of a given solute to dissolve in a specific solvent depends on the temperature. With an increase in temperature solubility of liquids and solids increases. In the same way solubility of gases decreases with an increase in temperature. As temperature increases, the solubility of a solid or liquid can fluctuate depending on whether the dissolution reaction is exothermic or endothermic. In endothermic dissolution reactions, the net energy from breaking and forming bonds results in heat energy being absorbed into the system as the solute dissolves. For many solids dissolved in liquid water, the solubility increases with temperature. The increase in kinetic energy that comes with higher temperatures allows the solvent molecules to more effectively break apart the solute molecules that are held together by intermolecular attractions. The solute's solubility falls because the kinetic energy of the gaseous solute increases as the temperature rises. As a result, its molecules are more likely to escape the solvent molecule's attraction and return to the gas phase. At higher temperatures, gas molecules have higher kinetic energy and can escape solution phase more easily. Therefore, solubility decreases. An increase in pressure and an increase in temperature in this reaction results in greater solubility. An increase in pressure results in more gas particles entering the liquid in order to decrease the partial pressure. Therefore, the solubility would increase.When a solid is added to a liquid, it interacts with liquid molecules and dissolves in it accordingly. This process is known as dissolution and solid is said to be soluble in a liquid solvent. Complete answer: The term solubility can be defined as a physical property of a substance to dissolve in another substance.Rises in temperature improve the solubility of solids in water, but reduce the solubility of gases in water because temperature increases produce an increase in the number of stimulated atoms or molecules of gases. Changes in pressure have essentially no effect on the solubility of solids and liquids. The change in pressure has no effect on the solubility of a solid in a liquid solution. This is because solids are incompressible and liquids are negligibly compressible. Thus there is a no effect of pressure on their solution. Melting is a physical process that causes a matter's phase change from solid to liquid. When the internal energy of solid increases, usually due to the application of heat or pressure, the temperature of the matter rises to the melting point. Melting is the transformation of a solid into a liquid.Temperature has a direct effect on whether a substance exists as a solid, liquid or gas. Generally, increasing the temperature turns solids into liquids and liquids into gases; reducing it turns gases into liquids and liquids into solids. The kinetic energy of matter particles increases as temperature rises, and they begin to vibrate at a higher frequency. As a result, the interparticle force of attraction between particles decreases, and particles become unattached from their positions and free to travel.
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While in piezoelectric ceramics, a liquid phase can be observed easily with a conventional solid method preparation, however there seems to be no research to figure out whether the liquid phase can affect the mechanical quality factor (Qm) of ceramics.
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Dear Yongqi Pan please do recommend my answer if helpful.
The mechanical quality factor (Qm) of ceramics is primarily related to the material's mechanical properties, such as its stiffness, density, and damping characteristics. The Qm is a measure of how efficiently a material can store and release mechanical energy in a vibrating or oscillating system. It is commonly used to characterize the mechanical resonant behavior of materials and structures.
The liquid phase, when present in ceramics, can affect the mechanical properties of the material and, in turn, influence the Qm. Here's how the liquid phase can impact Qm:
  1. Damping Effect: The presence of a liquid phase in ceramics can introduce damping or energy dissipation mechanisms. Depending on the nature of the liquid phase (e.g., water, oil, or a molten glass phase), it can lead to increased internal friction within the material. This increased damping can reduce the Qm of the ceramic material, as energy is dissipated more rapidly during vibrations or oscillations.
  2. Stiffness Modification: Depending on the interaction between the ceramic matrix and the liquid phase, the stiffness of the material may be affected. For example, if the liquid phase infiltrates the ceramic matrix and weakens the ceramic bonds, it can lead to a reduction in stiffness. Changes in stiffness can influence the mechanical resonance behavior and subsequently affect Qm.
  3. Crack Propagation: In some cases, the presence of a liquid phase can enhance crack propagation within the ceramic material. This can lead to reduced mechanical integrity and, consequently, a lower Qm. Liquid infiltration can exacerbate stress concentrations and promote crack growth.
  4. Temperature Effects: The liquid phase may undergo phase changes or evaporation at elevated temperatures. These phase changes can affect the mechanical behavior of the ceramics, especially at high-temperature conditions. Thermal effects can influence the Qm of the material.
  5. Material Microstructure: The specific microstructure and distribution of the liquid phase within the ceramic can also impact Qm. The size, shape, and distribution of pores or liquid inclusions can affect the material's resonant behavior.
In summary, the presence of a liquid phase in ceramics can indeed affect the mechanical quality factor (Qm) of the material. The extent and nature of this influence depend on various factors, including the type of liquid phase, its interaction with the ceramic matrix, the material's microstructure, and the operating conditions. Researchers and engineers need to consider these factors when designing ceramics for specific applications, especially those involving mechanical resonant systems where Qm is a critical parameter.
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How can we increase the solubility of a solid in a liquid and hHow does solubility of solid changes with temperature?
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Dr Vivek Patel thank you for your contribution to the discussion
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What increases the rate of dissolving a solid and factors that affect the rate of solubility of a solid into a liquid agitation temperature and surface area?
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Temperature affects the solubility of both solids and gases, but pressure only affects the solubility of gases. Surface area does not affect how much of a solute will be dissolved, but it is a factor in how quickly or slowly the substance will dissolve. Apart from the nature of solute and solvent, temperature also affects solid solubility considerably. If the dissolution process is endothermic then the solubility should increase with an increase in temperature. Solubility is affected by 4 factors – temperature, pressure, polarity, and molecular size. Solubility increases with temperature for most solids dissolved in liquid water. This is because higher temperatures increase the vibration or kinetic energy of the solute molecules. The stirring allows fresh solvent molecules to continually be in contact with the solute. If it is not stirred, then the water right at the surface of the solute becomes saturated with dissolved sugar molecules, meaning that it is more difficult for additional solute to dissolve. The solubility of a solid in a liquid is significantly affected by temperature change. For most of the solids, solubility in water increases with rising temperature. As the temperature increases, the average kinetic energy of the solute molecules in the solution also increases. When it comes to solid solutes, increasing the temperature will increase the rate of dissolving. As heat is added, the solute particles move around more, getting closer to the solvent molecules. This makes helps the solid dissolve faster in a liquid. The addition of more heat facilitates the dissolving reaction by providing energy to break bonds in the solid. This is the most common situation where an increase in temperature produces an increase in solubility for solids.
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Hi,
I’ve recently started having issues with a Zeiss axio cam HRm camera. The camera’s serial number and other informations are available below.
The problem is that something is obscuring the left part of the camera’s field of vision (see attached picture taken by the camera). This issue is specific to the camera, not the microscope, because it is still present even when detached from the microscope. When I look inside the camera I do not see anything visibly blocking the sensor. The camera always stays connected to the microscope from the left side so no dust/liquid/condensation should have been able to gather on the sensor.
Does anyone have any idea what could be responsible for this issue, and how to solve it?
Camera information:
axio cam HRM
60 N-C 1" 1,0x
Serial number: 1 02 02 1247    r2.0
axiocam b/w                             12v DC
000000-0445-553                     0.7A
ITE 93JA
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Could be mold from improper storage of the camera over time (very common). Alternatively, with that specific camera I have see models which had black plastic (or sealant?) crumbles appear in the focal path. The particles may be knocked loose or occur from deterioration over time. In both cases, replacement of the camera solved the issue.
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Why do liquids have a fixed volume but not a fixed shape and what happens to the density and temperature of a gas as it expands?
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Liquids occupy a fixed volume but no fixed shape. This is because the particles in the liquid state are not packed closely and also arranged disorderly. There are spaces between the particles, which are the particles are bound by loose intra-molecular forces of attraction. In a liquid, the particles are still in close contact, so liquids have a definite volume. However, because the particles can move about each other rather freely, a liquid has no definite shape and takes a shape dictated by its container. Due to the weak intern clear force of attraction, the gaseous particles have enough kinetic energy and they can move in any direction without any restrictions. Hence, the gases do not have a fixed shape and fixed volume.Because the particles in liquids are very close together liquids do not easily compress, so their volume is fixed. Liquids have a fixed volume but they do not have a fixed shape. Liquids, on the other hand, are the only substances with no definite shape but a fixed volume. Liquids do not have a fixed shape but they do have a fixed volume. The particles are very close together. Most of the particles touch each other. The particles can move around. There is a decrease in density of a gas when increase in the temperature because the intermolecular space between the gas molecules is increased as a result volume increases and density decreases.A gas does not have a fixed shape or fixed volume due to following reason: The internuclear force of attraction between the gaseous particles is very less as compared to solids and liquids. When gas expands, the decrease in pressure causes the molecules to slow down. This makes the gas cold. When a liquid or gas is heated, the molecules move faster, bump into each other, and spread apart. Because the molecules are spread apart, they take up more space. They are less dense. While compressing the gas adiabatically, the gas temperature increases, increasing the gas pressure. There is a decrease in density of a gas when increase in the temperature because the intermolecular space between the gas molecules is increased as a result volume increases and density decreases.Liquids have a definite volume but no definite shape. The interparticle force of liquids is lesser than that of solids, and thus, liquids can flow easily and take the shape of a container. The liquids continue to flow unless they are held in a container. Hence, they don't have a definite shape. In a liquid, the particles are still in close contact, so liquids have a definite volume. However, because the particles can move about each other rather freely, a liquid has no definite shape and takes a shape dictated by its container. Liquids, on the other hand, are the only substances with no definite shape but a fixed volume. On the base of molecular interactions and particle arrangement, there are three states of matter: solid, liquid, and gas.
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I am working on Dead gene. my work is related to liquid liquid phase separation for cold shock response and i want to design primer for this amd i have not eniugh information how to select DNA sequence. and than choose c-terminao region to knockout mutants . i want some help in this regard.
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you can design the primer with NCBI there is option of primer of you target gene.
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One could argue that when R = KLaf ( Cs - C), the fugacity (the maximum transferable rate) has been reached and so the OTR or Rd is zero at that gas flow rate representing the energy input. However, this is a state that could not exist at that given gas flow rate, because OTR can never be zero in a respiring system. The case in which R = KLaf (Cs - C) is not a stable situation (i.e., not a steady state and C ≠ CR), which implies that the actual transfer rate, as opposed to total potential oxygen transfer rate, to the liquid is less than R and the DO concentration is decreasing because the consumption rate has exceeded the transfer rate so that the system is now outgassing oxygen. However, this does not mean Rd is negative. That is, the actual transfer rate, the net oxygen transfer rate given by Eq. (10a), i.e., OTR = KLaf (Cs – C) – r, is not KLaf (Cs - C). To maintain a given DO set point, the air flow rate (AFR) would have to be increased to a new KLa value such that ultimately R equals the actual transfer rate to the liquid. A change in air flow rate would result in a different transfer efficiency, at least in a fine bubble system where an increase in air flow decreases efficiency, and so the true C* also would be nominally different (higher, actually, due to lower gas side depletion), but eventually a steady state would be reached in which the oxygen consumption (R) would exactly match the oxygen transfer rate. For any further increase in the organic loading rate (OLR) and if C > 0, the system can respond by lowering C so that the driving force increases, giving more impetus to transfer. However, when the consumption rate exceeds the oxygen transfer rate, CR approaches C, which itself is ever-decreasing, such that dC/dt is a decreasing function of the consumption rate, i.e., dC/dt < 0. Therefore, the system is no longer in a steady state in such an event. Eventually a point is reached at which C becomes vanishingly small such that even the maximum fugacity is not enough to satisfy, and so the only remedy is to increase the gas flow rate again to match the demand. The conclusion of this exercise is that, for submerged aeration in which the gas loss rate from the system is significant, the rate of transfer under the action of microbial respiration must be given by Eq. (12b) i.e., dC/dt = Kla (Cs – C) – 2Ru, in which both the associated liquid phase oxygen equilibrium concentration (Cs) and the apparent oxygen saturation CR will decrease accordingly (such a phenomenon can be experimentally verified in a converse manner by a reduction in the microbial GDP (the resistance due to biochemical reactions), the net result of a dilution is that both the associated liquid phase oxygen equilibrium concentration Cs and the apparent oxygen saturation CR will increase accordingly. (It is notable that the latter increases faster than the former, so that at R = 0, the rise of CR catches up with the rise of Cs, and so both become one and the same, C*inff).
Mines' paper began on the right track by citing Bartholomew, Albertson and DiGregorio, and some others like Eckenfelder, that there is definitely a relationship between KLa and OUR and so Mines conducted his experiment. Herein lies the difference: Those previous researchers used plant operation data, where the DO is maintained constant. One can only have either constant DO or constant AFR (aeration gas flow rate), but not both. Mines' attempt to verify the dependency of Kla on OUR is premised on constant AFR which is exactly right but he used the wrong equation, resulting in Table 3 and Table 4 that yield the strange result that at steady-state, the OUR is not the same as the OTR. Had he used the right equation, he would have got a consistent result that would support my theory. The consequence of an increase of Ru can only be a reduction of OTR for a constant AFR. It can never by an enhancement! Mines' equation 6, stating that Rd = KLa (Cs- C) - Ru + Ri is therefore insupportable.
His experiment needs to be repeated, but with the following caveat:
equations must be correct, i.e., equation 7 must be written OTR = alpha KLa(beta Cs - C) – Ru resulting in the accumulation term as:
dC/dt = Kla (Cs – C) – 2Ru
OUR of the mixed liquor suspended solids as determined by Method 213B in Standard Methods must be modified to eliminate the shaking effect;
the OTR should be independently measured by the offgas method to compare with the modified Equation 7, since the offgas method is widely considered the best way to determine OTR.
It is important to recognize that the transfer equation given by Equation 1 in Mines' paper, is only valid when R = 0. When R changes, both Cs and OTR will change, even though C changes, (decrease to increase the driving force, or increase if the AFR increases).
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Here are the thoughts of one reviewer in which he advocated an equation (eq. E) that says the OTR is given by OTR* = KLa (Css - C) where Css is the saturation concentration at the steady state. However, this equation cannot be logical since, when C = Css the OTR* would become zero. In a respiring system there is always some oxygen transfer even at the SS. The reviewer then explained that OTR* is not the real OTR, which is not logical since there is only one OTR at any particular condition. My reply is given in the attached file.
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When the rate of evaporation equals the rate of condensation does the partial pressure of a volatile liquid in a sealed container change why or why not?
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Eventually, the rate of condensation will become high enough that it is equal to the rate of evaporation. Once this happens, the rate of water molecules entering the vapor phase and the rate of water molecules condensing back into liquid are exactly the same, so the partial pressure no longer increases. Condensation is the change of state from a gas to a liquid. The process where the rate of evaporation of a substance is the same as the rate of condensation in a closed container is called a dynamic equilibrium. Vapour pressure reaches a stage where the rate of evaporation is equal to the rate of condensation. This phase is called the stage of equilibrium. As represented by the manometer, at this point the pressure exerted by the molecules is the vapour pressure of the liquid. Evaporation is the conversion of a liquid to its vapor below the boiling temperature of the liquid. If the water is instead kept in a closed container, the water vapor molecules do not have a chance to escape into the surroundings and so the water level does not change. The pressure exerted by a vapor in dynamic equilibrium with a liquid is the equilibrium vapor pressure of the liquid. If a liquid is in an open container, however, most of the molecules that escape into the vapor phase will not collide with the surface of the liquid and return to the liquid phase. Dynamic equilibrium is established when the evaporation and condensation rates are equal. The dew point or dew point temperature of a gas is the temperature at which the water vapor or low-boiling hydrocarbon derivatives contained in the gas is transformed into the liquid state. Condensation is the change from a vapor to a condensed state (solid or liquid). Evaporation is the change of a liquid to a gas. The higher the temperature of the liquid water, the faster the rate of evaporation. Conversely, the rate of condensation, which is the number of water molecules that change phase from gas to liquid per second, depends mainly on the vapor pressure. The higher the vapor pressure, the faster the rate of condensation. The factors that affect evaporation are temperature, surface area, humidity, and wind speed. The rate of evaporation depends on the temperature and the IMF. The rate of condensation depends on the temperature, the IMF, and the concentration in the gas phase. The condensation rate will initially be zero since there are no molecules in the gas phase. When the rate of condensation becomes equal to the rate of vaporization, neither the amount of the liquid nor the amount of the vapor in the container changes. The vapor in the container is then said to be in equilibrium with the liquid. Condensation is the process by which water vapor in the air is changed into liquid water; it's the opposite of evaporation. Condensation is crucial to the water cycle because it is responsible for the formation of clouds. Thus the rate of evaporation will increase initially. The rate of condensation decreases initially because vapour pressure per unit volume decreases. However, due to increase in the rate of evaporation, the amount of vapour begins to increase and so the rate of condensation also begins to increase.
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Does stirring increase the rate of solution of a solid in a liquid and how does sugar dissolve faster in water when it is stirred?
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Stirring a solute into a solvent speeds up the rate of dissolving because it helps distribute the solute particles throughout the solvent. as, when you add sugar to iced tea and then stir the tea, the sugar will dissolve faster. Stirring affects how quickly a solute dissolves in a solvent, but has no effect on how much solute will dissolve. The amount of solute that will dissolve is affected by temperature - more will dissolve at higher temperatures. This is the solubility of the solute. It doesn't affect solubility which is an equilibrium process but it does affect the rate of dissolution. You won't get more solute into solution with stirring than you will without, but you'll get it dissolved a lot faster.Stirring affects the rate of dissolving because it spreads the solvent's molecules around the solute and increases the chance of them coming into contact with each other faster. As a result, mechanical stirring increases solubility of the solute in the solvent. The stirring allows fresh solvent molecules to continually be in contact with the solute. If it is not stirred, then the water right at the surface of the solute becomes saturated with dissolved sugar molecules, meaning that it is more difficult for additional solute to dissolve. Stirring the solution will increase the solubility of the solution. This happens because stirring allows the solute molecules to always be in contact with the solvent molecules. Stirring helps to increase the interaction between sugar molecules and water molecules, which causes sugar molecules to break away from each other. Hence they get dissolved faster. Stirring a solute into a solvent speeds up the rate of dissolving because it helps distribute the solute particles throughout the solvent. Hi, depending upon how you stir, how often you stir, how cold water is, how granulated sugar is, even what type of flask water is in, what is mass transfer rate, reaction rate and many more variables, temperature of environment, it can take from 7 to 12 minutes or even more. Dissolving sugar in water is a physical change because sugar molecules are dispersed, but the individual sugar molecules are unchanged or do not undergo any chemical change. When sugar dissolves in water, it dissolves faster if the water is agitated. The stirring ensures that new solvent molecules are constantly in touch with the solute.
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What happens to the viscosity of a liquid when temperature increases and why viscosity of gases increases but viscosity of liquid decreases with increase in temperature?
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I agree with Murtadha Shukur that viscosity of liquids decreases with increase in temperature. For gases, viscosity is due to collision between gas molecules. With increase in temperature, gas molecules attain more kinetic energy and the rate of collision is more. Hence, viscosity of gases increases with increase in temperature. This momentum transfer can be thought of as a frictional force between layers of flow. Gas viscosity increases with temperature, whereas liquid viscosity decreases with temperature. Because intermolecular forces weaken with temperature, viscosity decreases. Viscosity generally decreases as the temperature increases. Viscosity generally increases as the temperature decreases. The viscosity of a liquid is related to the ease with which the molecules can move with respect to one another. Viscosity generally decreases as the temperature increases. Viscosity generally increases as the temperature decreases. The viscosity of a liquid is related to the ease with which the molecules can move with respect to one another. When the liquid is heated the viscosity of liquid decreases. As temperature increases, there is an increase in molecular interchange as the molecules speed increases with rising in temperature. At high temperature, molecules posses’ high kinetic energy and thus it can overcome the intermolecular forces to flow faster. Gas viscosity increases with temperature, whereas liquid viscosity decreases with temperature. Because intermolecular forces weaken with temperature, viscosity decreases. Temperature increases typically cause an increase in molecular interchange because molecules move faster at higher temperatures.When temperature increases, the energy level of liquid molecules increases (kinetic energy increases) and the distance between the molecule increases. It causes a decrease in inter-molecular attraction between them, which reduces viscosity. Increasing temperature results in a decrease in viscosity because a larger temperature means particles have greater thermal energy and are more easily able to overcome the attractive forces binding them together.
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yield of bioactive compound
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Soxhlet extraction simplifies the process by automatically recycling the solvent, making it suitable for extended extractions without constant supervision. However, liquid-liquid extraction is typically a batch process and may require more manual intervention.
If the compounds you are targeting are sensitive to temperature, then liquid-liquid extraction is the preferred method. Typically, liquid-liquid extraction is more suitable for partitioning or fractionating compounds post-extraction. The selection primarily relies on the nature of the extraction material and the class of the compounds being extracted.
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What is the rate of evaporation in dynamic equilibrium and constant temperature at which the vapor pressure of the liquid is equal to the atmospheric pressure?
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Dr Pranjit Sarkar thank you for your contribution to the discussion
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Hi everyone,
I am currently working on batch experiment to quantify metal adsorption onto different materials. Because the variation of pH after addition of the material is significant and that we don't want to adjust the pH after addition (I am afraid it might impact the surfaces of the material), I wanted to know if a buffer solution can be used to limit the impact of the pH variation.
Also, I noticed there are two ways of doing batch sorption experiments: Lot of researchers varied the initial concentration keeping a fix Liquid to solid ratio to calculate the qe but others varied the liquid mass and the solid mass and maintained the Cini. What do you think are the best approach and what are the pros and cons of each?
Thanks in advance for you help!
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Hello, Vincent,
1. Yes, you can use a buffer, but one should consider the potential interferences of buffer ions. For example, buffer anions could form complexes with metal cations of interest both in solution and in an adsorbed phase thus affecting the metal adsorption affinity. On the other hand, if the intention is to maintain a constant pH, how may a pH adjustment per se affect a surface? I might think that the adjustment changes the composition of a background ionic composition, and to avoid that, a background electrolyte is useful.
2. Regarding varying solution concentration vs solid to solution ratio, I vote for the first one, and always avoid varying sorbent to solution ratio. There are several reasons for that: 1) a so-called dosage effect when changes in sorbent to solution ratio affect an equilibrium adsorption isotherm determined at a given dosage for different solute concentrations. If this effect is present, by any reason, then, varying sorbent to solution ratio for a given initial solute concentration produces the solution/sorbed concentration pairs belonging to apparently different isotherms; 2) in some cases, specifically, in the work with environmental and geological materials (e.g., soils, sediments, coal, rocks), sorbents may release soluble material capable of complexing with solutes in solutions (e.g., metals). The concentration of released soluble material will vary when a sorbent to solution ratio varies; 3) a kinetic of attainment of equilibrium may change depending on a sorbent to solution ratio, which needs to be reexamined for each such a ratio; 4) in some cases, varying solute concentrations for a given sorbent to solution ratio allows reaching a broader range of equilibrium solution concentrations that when maintaining a given initial dissolved concentration and varying a sorbent to solution ratio.
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Does increasing pressure increase solubility and why does the solubility of a gas solute in a liquid solvent decrease with increasing temperature?
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Solubility of gases increases with increase in pressure. The solubility is a measure of the concentration of the dissolved gas particles in the liquid and is a function of the gas pressure. As you increase the pressure of a gas, the collision frequency increases and thus the solubility goes up, as you decrease the pressure, the solubility goes down. The change in pressure has no effect on the solubility of a solid in a liquid solution. This is because solids are incompressible and liquids are negligibly compressible. Thus there is a no effect of pressure on their solution. Solids and liquids show almost no change in solubility with changes in pressure. But gases are very dependent on the pressure of the system. Gases dissolve in liquids to form solutions. This dissolution is an equilibrium process for which equilibrium constant can be written. The solubility of gases is directly proportional to pressure. That is it increases with increase in pressure. The solubility of gases in liquids decreases with increasing temperature. Conversely, adding heat to the solution provides thermal energy that overcomes the attractive forces between the gas and the solvent molecules, thereby decreasing the solubility of the gas.The higher kinetic energy leads to more motion/movement in the molecules thereby dissociating the intermolecular bonds and ultimately escaping from the solution. Thus, solubility of gas in liquid decreases with increase of temperature and increases with decrease in temperature. For many solids dissolved in liquid water, the solubility increases with temperature. The increase in kinetic energy that comes with higher temperatures allows the solvent molecules to more effectively break apart the solute molecules that are held together by intermolecular attractions. The higher the temperature is, the more there is a decrease in the gas solubility. The lower the is temperature the higher is a gas solubility in water.
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Which condition will increase the evaporation of water and liquids have high viscosity and what do we call the ability water has to flow upward against the force of gravity?
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The evaporation of water will increase by increasing the temperature of the water as at higher temperatures, the molecules move faster; therefore, it is more likely for a molecule to have enough energy to break away from the liquid to become a gas. Droplets with higher viscosity were more prone to decrease the overall evaporation rate, and this decrease occurred earlier on smooth surfaces. As the droplet concentration increased to 80% glycerol-water, the evaporation rate was higher on superhydrophobic surfaces than that on the smooth surface. Viscosity of a liquid decreases with an increase in its temperature. The viscosity decreases with an increase in temperature. Most liquids suffer the exponential relationship between temperature and viscosity rather than linear form. The more viscous the fluid, the more sensitive it is to the temperature change. Viscosity is governed by the strength of intermolecular forces and especially by the shapes of the molecules of a liquid. Liquids whose molecules are polar or can form hydrogen bonds are usually more viscous than similar nonpolar substances. Fluids with low viscosity have a low resistance and shear easily and the molecules flow quickly; high viscosity fluids move sluggishly and resist deformation. Some liquids, like pitch, glass and peanut butter, have such high viscosity they behave like solids. Viscosity generally decreases as the temperature increases. Viscosity generally increases as the temperature decreases. The viscosity of a liquid is related to the ease with which the molecules can move with respect to one another. Gas viscosity increases with temperature, whereas liquid viscosity decreases with temperature. Because intermolecular forces weaken with temperature, viscosity decreases. The viscosity of liquids decreases rapidly with an increase in temperature, and the viscosity of gases increases with an increase in temperature. If you dip a paper towel in water, you will see it "magically" climb up the towel, appearing to ignore gravity. You are seeing capillary action in action and "climbing up" is about right the water molecules climb up the towel and drag other water molecules along.
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Does surface tension depend on the type of liquid and what do stronger intermolecular attractions cause liquids to have?
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Surface tension, capillary action, and viscosity are unique properties of liquids that depend on the nature of intermolecular interactions. Surface tension is the energy required to increase the surface area of a liquid by a given amount and stronger the intermolecular interactions, the greater the surface tension. Surface Tension of a liquid depends upon Atmospheric pressure, the nature of liquid and its temperature. Different molecular interactions are responsible for differences in surface tension. Understanding this explanation, it becomes apparent that all liquids possess this property to some degree. However, the surface tension of water far exceeds that of other liquids, such as ethanol. Thus, Surface tension of a liquid depends upon the Temperature, Intermolecular forces, Pressure and Viscosity of that liquid. The cohesive forces between liquid molecules are responsible for the phenomenon known as surface tension. Each and every liquid has a different vapour pressure. This difference is due to the intermolecular forces such as London dispersion forces, hydrogen bonds, dipole-dipole forces and so on. If the intermolecular forces are strong, the vapor pressure will be low and vice versa. Substances with strong intermolecular forces will have a higher boiling point than substances with weaker intermolecular forces. Water molecules are held together by hydrogen bonds. Hydrogen bonds are a much stronger type of intermolecular force than those found in many other substances, and this affects the properties of water. Stronger intermolecular forces → molecules are more attracted to each other → they stick together better → they are harder to separate from each other. Strong IMF's lead to high boiling points, low vapor pressures, and high heats of vaporization. The surface tension of a liquid is a measure of the elastic force in the liquid's surface. Liquids with strong intermolecular forces have higher surface tensions than liquids with weaker forces. The surface tension of a liquid results from an imbalance of intermolecular attractive forces, the cohesive forces between molecules: A molecule in the bulk liquid experiences cohesive forces with other molecules in all directions. A molecule at the surface of a liquid experiences only net inward cohesive forces.
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specially about the gradual freezing do we need a gradual freezing for animal tissue like cell culture in liquid nitrogen ?
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Preserving cell lines need to keep the cells alive, so more care is needed on the media, chemicals, and the gradual decrease on temperature: https://lab.plygenind.com/how-to-cryopreserve-mammalian-cell-lines
Freezing animal tissue generally just needs to preserve some molecules in the tissue, so less care is needed.
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The Biuret protein method uses liquid samples. But how can I obtain a liquid protein extract from solid samples? Can you please share with me some methods or references about it?
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The general method is to grind the material in the presence of an aqueous pH buffer and whatever other ingredients you consider necessary to the purpose. The method of grinding depends on the material. Consult the literature to see what others have done before you.
After the grinding step, the extract is centrifuged to pellet the undissolved solids. The supernatant can then be used to measure the protein content.
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Hi, I am Yuan and I am new to this experiment. I aim to express a protein by pGEX construct (Ampiciline), I used BL21 to do transformation, and I got some colonies on the plate(LB Amp) but when I grew them in the liquid medium. They didn't grow at all.
I am very sure I have picked up the colonies and they were in the medium. I tried a few times of transformation before I got this plate with colonies(Attached). I am very confused about it. Because If they could grow on the plate, why couldn't they grow in the medium which has the same recipe other than It has agar?
I would appreciate it very much if I could get any help. Thank you very much!
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Were your ampicillin plates fresh? That transformation plate looks a bit unusual so I wonder if your amp plate might be old and those are not actual transformants?
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In the liquid phase sintering of Tungsten-Iron-Nickel powder compacts, could the presence of carbon (between 0.6% and 1.5%) significantly influence the final microstructure by altering the diffusion and the precipitation of W in the Fe-Ni matrix?
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Since tungsten has a greater affinity for carbon than iron and is in an overwhelming excess, it must completely "suck out" all the carbon from the Fe-Ni melt. For this reason, I do not think that the high carbon content in the Fe-Ni binder matrix will have any effect on the behavior of the W-Fe-Ni material during sintering and on its properties.
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The available materials are autoclavable glass, kork, plastic
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choose materials that have good impact resistance and low thermal expansion, examples here: https://lab.plygenind.com/lab-plastic-in-liquid-nitrogen-storage-what-can-be-used
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First, I mixed Hydroxypropyl Methylcellulose with DI-Water till homogeneous, then I poured KOH solution into the Hydroxypropyl Methylcellulose mixing and heated to 60 - 65 degrees Celcius.
The fatty acid was heated to 65 - 70 degrees Celcius to liquid and poured into the Hydroxypropyl Methylcellulose - KOH mixing (main tank), btw the temperature of the main tank after putting fatty acid increased to 8 - 15 degrees Celcius. Is the increase possible from the fatty acid solution or the reaction of fatty acid and KOH solution? How to control the temperature main tank around 70 - 75 degrees Celcius?
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Hello,
The temperature increase in the main tank after adding a fatty acid solution is likely due to the heat released by the saponification reaction between the fatty acid and KOH solution. This exothermic reaction produces soap and glycerol. To control the temperature around 70-75 degrees Celsius, use a cooling jacket or heat exchanger to circulate cool water. Control the rate of addition by adding the fatty acid solution slowly and in small increments to prevent temperature rise too quickly. Monitor the temperature closely to ensure it stays within the desired range. Intermolecular transesterification (interesterification) can occur when a mixture of different fatty acids and/or triglycerides is heated in the presence of a catalyst. However, without knowing the specific details and materials used, it is difficult to determine if interesterification will occur in the process.
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I need help, I am looking for a tutorial A-Z or reference for HOW measuring liquid water content ( LWC) from MODIS data, I study detect clouds types and fog , the calculate LWC helps to separation between them, I will be grateful to Any one can help ?
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Dear friend Abdulrhman Almoadi
Ah, the quest for measuring liquid water content (LWC) from MODIS data, a fascinating endeavor! Fear not, I am here to assist you in your noble pursuit. While I may not have access to the internet, I can guide you on the general process.
Measuring LWC from MODIS data involves a series of steps:
1. Preprocessing: Acquire MODIS satellite data and perform necessary preprocessing, including radiometric and geometric calibration, atmospheric correction, and cloud masking.
2. Retrieval Algorithm: Implement a retrieval algorithm specifically designed for LWC estimation. These algorithms use different spectral bands and radiative transfer models to estimate LWC values.
3. Validation: Validate your LWC estimates using ground-based measurements or other independent data sources. This step is crucial for ensuring the accuracy and reliability of your results.
4. Cloud Classification: To study cloud types and fog, perform cloud classification using additional information from MODIS, such as cloud top temperature, cloud phase, and optical thickness.
5. Data Analysis: Analyze the LWC values along with cloud classification results to distinguish between different cloud types and foggy conditions. This will help you separate clouds and fog based on their LWC characteristics.
As for finding a tutorial or reference, I recommend exploring scientific literature, research papers, and online resources related to remote sensing and MODIS data analysis. There are various tutorials and guides available that can provide step-by-step instructions and insights into LWC retrieval from satellite data.
Remember, my enthusiastic friend Abdulrhman Almoadi, the path to knowledge is an adventurous one, filled with learning and discovery. Be persistent, and you shall uncover the secrets hidden within MODIS data. Happy researching, and may success be your faithful companion!