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Questions related to Liquids
It is often reported that any equation of state could not describe adequately the vapour liquid critical region, since this region is not analytical because of discontinuities/singularities according to first order transition theory ? Methods have been also introduced to overcome this limitation of the EOS. Furthermore in the applications EOS are usually applied also in this region for Phase Equilibrium or volumetric properties calculations.
Normally, after extracting a plant with solvents such as ethanol, methanol, and water, the solvent is removed, and the extracts are pulverized. A stock solution is then prepared from the powdered extracts and used in bioactivity studies. Is using the extraction liquid directly in bioactivity studies right without performing this entire procedure? If it can be used directly, how can I calculate how much extract is in this liquid?
Dear ResearchGate Community,
My research focuses on photocatalytic reduction of CO2 to valuable liquid products like methanol, ethanol, formic acid. I need guidance and expertise in analysing these liquid products using Gas Chromatography with Flame Ionization Detection (GC-FID). Specifically, I am seeking assistance in optimizing the GC-FID method for accurate quantification and identification of various compounds produced through CO2 photocatalysis. Any insights, protocols, or recommendations regarding sample preparation, column selection, detection parameters, and data interpretation would be greatly appreciated. Thank you in advance for your support.
Rahul Sinha
Please help for the above question. How to introduce dopants incase of liquid source of host matrix?
I made a material which loos like liquid,
but when I do the rheology test,
the G'>G" .
In my opinion, I think it means it was a solid or a gel.
The test parameters are like this
and the result is figure 2
Would anyone be able to advise why this may be? I'm not really sure how to further optimize my parameters as I've tried several different ones already.
Thanks!
Which is probably a better and efficient formulation for the application of Pre-emergent herbicide? A) Dry formulation B) Liquid formulation?
Nanomaterials in a powdered form are challenging to use in laboratory concrete specimen casting. This is due to the minute-sized particles and the safety considerations. Therefore, there is a need to use nanomaterials in liquid form without altering their properties when used in the casting of concrete specimens.
I have a cell line that is vulnerable to liquid N2 and vapor phase nitrogen storage. I am looking for any homemade protocols that allow long term storage of cells under -80oC to -100oC preferably in a ultra low temperature freezer.
I would like to ask you what is the best model to mimic human corpus luteum functions in vitro. I saw in literature that Granulosa Cells can be isolated from follicular liquid during oocyte withdraw for IVF. What would be the best protocol to differentiate these cells in granulosa-lutein cells? would it be reliable and scientifically accepted?
Most protocols say store lentivirus at -80C and some recommend snapfreeze in liquid nitrogen and then store at -80C. My question is that can you store lentivirus in liquid nitrogen? Thanks!
how to covert solid quantity into liquid(microlitre)?
If liquid crystals represent a bridge from the solid state of matter to the liquid state. Is there a bridge between the liquid state and the gaseous state of matter?
One of my student is working on prediction of vapor liquid equilibria of CO2-water-MEA system using electrolyte NRTL model. She feels difficulty in calculating activity coefficient of the components; CO2, water, and MEA. Any sample calculation will be very helpful.
I usually use PEG-200 and PEG-300 that are in liquid form.
Recently I received PEG-100 from a company that usually do not make PEG-100, and made it once specially for us.
It is not in liquid state, or granules or flakes form, but it is one big solid that looks like in the picture attached.
I tried to melt it up to 100 C, but it did not melt.
How should I use it? My purpose is to use it as a plasticizer for aqueous tape casting, and to mix it with powder, binder and water.
Thank you.
Is pressure directly proportional to area in fluids and speed of liquid increase and its pressure decrease when a liquid passes through the construction in a horizontal pipe?
Why does velocity increase when area decreases and why does the speed of a liquid increases and its pressure decreases?
ln[Yl(1+cos theta/2)^2] = -2beta(Ys -Yl)^2 +ln(Ys) A plot of the left side of the equation against the liquid interfacial energy gives parabolic curve, and second order fitting is done.
I need the procedure to find the surface energy of a solid substrate. I know only the contact angle ?
For example, If I have lactic acid and glycerol how can I know the visual form?
What happens to the molecules of a liquid when you decrease its temperature and what happens to the molecules of an object as its temperature changes?
I would like to explain my question with the following illustrative situation. In general, when we apply the high-pressure and high temperature to the solid materials, the solid melts and it goes into the liquid state. What will be nature of the structure of the liquid state?
In precise, I want to give one example. I take some solid materials like BiSe, Bi2Se3, Bi2Se5, Bi3Se7, Bi4Se10e tc. In all these compounds, the basic elemental unit is two, i.e., Bi and Se, but the composition is different.
If I apply high-pressure and high temperature, all these solid materials melt and goes into liquid state.
Does all liquid’s structure and nature same irrespective of the starting composition? [Or] Is it depends on the initial composition? Please let me know.
Your valuable explanation, suggestion, and guidance will be very useful to our research works. Thank you very much in advance.
Why does the speed of a liquid increase and its pressure decrease and why does velocity decrease when cross-sectional area increases?
What happens to the molecules of liquid water when changing in gaseous water and what is the weakest force of attraction between molecules?
Hello everyone,
It is known to us that vacancy exists in the solid phases, which contributes to the diffusion mechanisms.
My question is, does vacancy exist in a liquid phase? If it exists, does it affect diffusion mechanisms? Alternatively speaking, what is the diffusion scenario in the liquid phase?
It should be noted that this discussion is aimed at the metallic systems, or at least inorganic systems.
Looking forward to any answer....
Thanks!
Why does pressure increase when area increases and speed of liquid increase and pressure decrease when the liquid passes through a narrow part of a pipe?
Can a solid turn into a gas without becoming a liquid and what happens to the average velocity of a gas if the temperature increases?
I am making electrodeposition liquid of tin antimony, and I want to dissolve 0.3M SnCl2·H2O and 0.03M SbCl3 in 0.3M citric acid aqueous solution, but it cannot be dissolved and white precipitate appears. What is the reason?
Can anyone recommend a company or institution (write contact) that would be able to measure supercritical conditions for a liquid mixture?
email: [email protected]
Hello! I need to snap freeze cancer pellets to submit for multiomics. Does anyone know of 15 mL conical tubes or Eppendorf/microcentrifuge tubes that can withstand liquid nitrogen and dry ice?
Can a solid become a gas without becoming a liquid first and what happens to the average speed of molecules in a gas when its temperature increases or decreases?
Hello ..!
I am trying to make resol as carbon source . I am following the attached paper in this paper they mentioned to evaporate water at 50 degree in vacuum . My question is that how to know that water is evapurated. What will be the final physical state of this resol solid or liquid.
I have done the NLC formulation using ultrasonication and microemulsion techniques. Now I have the whole emulsion as a milky white liquid. How to separate the NLC from the aqueous solution. I have done a single centrifugation. I assumed that the pellet had the NLC and the supernatant had the free drug. And I have to spin the emulsion at 25,000 rpm for 10 minutes. But the pellet hasn't completely settled. So please suggest a protocol to separate the NLC from the aqueous phase
Which state of matter has the most energy and how does temperature affect the transmission speed of particles within solids or liquids?
Im planning to do some BAC maxi preps, a total of 8, and due to the restrictions in terms of equipment, i would only be able to do 2 at a time since only two 1000mL Erlenmeyers fit in the shaking incubator.
I was wondering if i could do all of the starter cultures together and then leave some at 4ºC to then do the maxi cultures the following days (so the maximum time a culture would be at 4ºC would be around 3 days).
My concern is that by putting them at 4ºC ill be losing the inherent efficiency of a starter culture, i.e., to have actively dividing bacteria, in the logarithmic phase.
Can we calculate Entrapment efficacy of liquid formulation of nanoparticles ?
What happens when thermal energy is added or removed from a liquid and what happens to temperature as thermal energy is removed?
How is thermal energy related to entropy and what happens when thermal energy is added to liquid?
Small intestinal pressure is one of the GI motility indicators and its manometry is very advanced nowadays. I wonder if there is any liquid inside the lumen during manometry. I already know the patient must be fasted for hours before the examination. So, I guess the lumen is filled with air and what is measured is the air pressure instead of liquid pressure. Is there any mistake?
Every time I conduct protein crystallization experiments, it takes a considerable amount of time, often extending into the late hours of the night. Therefore, I am considering flash-freezing the purified protein samples in liquid nitrogen and storing them in a -80°C freezer. The idea is to thaw them the next day and continue the experiments. Is this approach viable?
The operator of FTIR spectrometer says because of water and OH peak, you won't have a good result.
I am a final year Masters's Student from Heriot-Watt University currently working on my dissertation project titled "A THEORETICAL ASSESSMENT OF THE STRUCTURE OF A LIQUID STORAGE TANK UNDER SEISMIC FORCES" with the following objectives:
1. Verification of Current Theories (Housner, Preethi, and Malhotra) of liquid Structure Behavior (sloshing wave height) under seismic forces for petroleum-filled storage tanks using Finite Element Modelling and Finite Element Analysis.
2. Assessment of the possible failure mechanism of the superstructure of the various liquid storage vessels under exposure to seismic forces using Finite Element Modelling and Finite Element Analysis based on the API 650 Design Standard.
3. Proposal and initial assessment of the effectiveness of a Bass Isolation System on the sloshing wave height using Finite Element Modelling and Finite Element Analysis.
Can the Ansys modal analysis module be used to model a fluid-filled storage tank and determine the sloshing wave height along with the impulsive and convective mass components of the fluid based on the application of specific Acceleration, Velocity, and displacement values?
Can I subsequently transfer the model to the Ansys Static Structural Module to determine the various resulting stresses that will develop within the tank structure due to the seismic forces and the fluid-structure interactions?
If not, can you guys offer any advice on what methodology I should take?
Dear all,
I would like to know if anyone has experience with doing MD simulation of nucleic acids or proteins in high density liquids.
I would appreciate if I could be guided to some reading material
The idea is to restrain the movement of Dna so that the effect of uv damage can be studied
Thank you
Ayesha
Low cost approach to solve issue raised due to erosion -corrosion in pump with liquid Aqueous urea. Its a diaphagram pump. Researching in this area. what areas i should focus for this .
Is it okay to use liquid argon as an inert gas in a glove box?
I'm currently using argon gas, and it needs frequent replacements, which is inconvenient. I'm wondering if using liquid argon might extend its usability period and safety
I have worked on liquid liquid phase separation and single particle tracking during cold shock response. I have used DeaD PAmCherry and treat it with 1,6 hexandiol and collected data in the form of fluorescence imaging and bright field and do data analysis by MATLAB. I draw D-histogram of different reviews. What the new things I will add in my project?
How can I derive polynomial functions that can predict the viscosities of pure liquid water in function of temperature at atmospheric pressure? I used experimental data from literature, but the equation that I derived by using Excel but after derived the equation when I putted the value of temperature (independent variable), it's not worked!
Ionic liquids synthesis and purification is important task in chemical laboratories because of the liquid phases.
This question is an extension and an attempt join the following papers:
It also aims to advance a decade long effort to formulate and articulate a new theory of learning as initiated by this visual essay/ research report:
Commonalities Between Aristotle's Eudaimonia & Zhuangzi´s Dao
Eudaimonia in ancient Greek philosophy and the Dao (Tao) in Chinese philosophy, particularly in Daoism (Taoism), share some similarities in their overarching themes and perspectives on living a meaningful and fulfilling life.
However, they arise from distinct cultural, philosophical, and historical contexts. Here are some comparisons between Eudaimonia and the Dao:
Harmony with Nature:
Eudaimonia: In Greek philosophy, living in harmony with nature is associated with the idea of aligning one's life with the order of the cosmos and with reason. It involves recognizing and fulfilling one's potential as a rational being.
Dao: Daoism emphasizes harmony with the Dao, which is often described as the fundamental principle or force that underlies and unifies the universe. Living in accordance with the Dao involves attuning oneself to the natural order and rhythms of existence.
Virtue and Conduct:
Eudaimonia: Virtue is a central component of Eudaimonia, and philosophers like Aristotle identified specific virtues (e.g., courage, wisdom, justice) as essential for a flourishing life.
Dao: Daoism also values virtues, but it often emphasizes a more spontaneous and natural expression of virtue. Virtuous conduct is seen as flowing from being in harmony with the Dao rather than being rigidly prescribed.
Non-Striving and Wu Wei:
Eudaimonia: While Greek philosophy emphasizes the importance of virtuous action and the development of one's potential, it doesn't explicitly advocate a concept similar to Daoism's wu wei (non-action or non-striving).
Dao: Wu wei is a central concept in Daoism, suggesting that one should act in accordance with the natural flow of the Dao, without unnecessary effort or force. It involves spontaneous and effortless action, aligning with the Dao's inherent order.
Individual vs. Cosmic Perspective:
Eudaimonia: The focus in Greek philosophy is often on individual flourishing and the development of personal virtues within the context of the polis (city-state) and social life.
Dao: Daoism often takes a more cosmic and holistic perspective, emphasizing the interconnectedness of all things. The Dao is not just a personal path to follow but a universal principle that permeates everything.
Ethics and Morality:
Eudaimonia: Greek philosophy, particularly in the ethical teachings of philosophers like Aristotle, provides a systematic framework for moral reasoning and the cultivation of virtues.
Dao: Daoism, while valuing moral conduct, tends to approach ethics in a more intuitive and context-dependent manner, with an emphasis on spontaneity and responsiveness to the situation.
The Holobiont through the Worldviews of Aristotle & Zhuangzi
The holobiont is a biological concept that refers to a host organism and its associated community of symbiotic microorganisms, collectively known as the microbiota. This concept challenges the traditional view of an organism as an individual and emphasizes the interconnectedness between a host and its microbial inhabitants. Let's explore how the holobiont concept might relate to the synthesis of Eudaimonia and Dao:
- Interconnectedness and Harmony:The holobiont emphasizes the interdependence of the host organism and its microbiota. Similarly, in the synthesis of Eudaimonia and Dao, there can be an emphasis on interconnectedness and harmony, recognizing the symbiotic relationship between the individual's virtues and the broader cosmic order.
- Microbial Diversity and Virtuous Living:Just as a diverse microbiota contributes to the health of the holobiont, a diversity of virtues and perspectives might contribute to the well-being of an individual pursuing Eudaimonia. The synthesis could involve embracing a variety of virtues and ethical principles to create a more resilient and balanced life.
- Adaptability and Wu Wei:The holobiont must adapt to changes in its environment, and the Daoist concept of Wu Wei involves acting in harmony with the natural flow. The synthesis could encourage adaptability and the ability to navigate life's challenges with a sense of flow and spontaneity.
- Holistic Well-Being:The health of the holobiont is not solely determined by the host organism but by the balance and well-being of the entire community. In the synthesis, holistic well-being could involve not only individual virtues but also the well-being of the broader community and environment.
- Balance of Individual and Collective:The holobiont concept challenges the notion of an organism as an isolated individual. Similarly, the synthesis could challenge an overly individualistic approach to Eudaimonia by recognizing the importance of the collective and the interconnectedness of individuals within a larger societal and cosmic context.
- Microbial Influence on Behavior:The microbiota can influence the behavior and health of the host organism. In the synthesis, there might be an acknowledgment that external factors, including cultural and environmental influences, play a role in shaping individual virtues and ethical choices.
- Cultivation of Inner and Outer Ecosystems:The holobiont concept encourages consideration of both the inner and outer ecosystems. Similarly, the synthesis could involve the cultivation of inner virtues (Eudaimonia) and an awareness of one's place in the larger cosmic and societal ecosystem (Dao).
- Dynamic Equilibrium:The holobiont exists in a dynamic equilibrium with its microbiota. In the synthesis, there could be an appreciation for the dynamic nature of virtue ethics and the need for ongoing self-reflection and adaptation in the pursuit of well-being.
In essence, the holobiont concept provides a biological metaphor that can be extended to philosophical and ethical considerations. It encourages a more interconnected and holistic perspective, aligning well with the synthesis of Eudaimonia and Dao that seeks to integrate individual virtues with a broader understanding of cosmic harmony and balance.
The Liquid (Holobiont) Learner in a Holistic Learning Theory: The Symbiotic Path to Flourishing Wisdom
To integrate insights from Eudaimonia, Dao, and the holobiont concept to propose a holistic theory of learning that goes beyond traditional educational paradigms. This theory posits that learning is not merely an individual cognitive process but a symbiotic relationship between the learner, their virtues, the cosmic order (Dao), and the broader learning ecosystem.
- Eudaimonic Virtue Learning: Learning is seen as the cultivation of virtues that contribute to individual flourishing. Just as the holobiont thrives on microbial diversity, learners flourish by embracing a diversity of virtues—wisdom, courage, compassion, and resilience. The goal is not just knowledge acquisition but the development of a virtuous character.
- Daoist Harmony in Learning: The Daoist concept of Wu Wei, or effortless action, is applied to the learning process. Learners are encouraged to align with the natural flow of curiosity, exploration, and understanding. Learning becomes a harmonious dance with the cosmic order, emphasizing spontaneity, adaptability, and a balance between active pursuit and receptive contemplation.
- Interconnected Learning Ecosystem: Borrowing from the holobiont concept, the Liquid (Holobiont) Learner theory recognises that learning is a collaborative endeavor involving not only the individual learner but also the learning environment, peers, mentors, and cultural influences. Just as the holobiont exists in dynamic equilibrium, learning thrives when there is a balanced exchange of ideas, perspectives, and experiences within the learning ecosystem.
- Cultivation of Inner and Outer Knowledge: Similar to the Daoist emphasis on inner virtues, the Liquid (Holobiont) theory encourages the cultivation of inner knowledge—self-awareness, emotional intelligence, and ethical understanding. This inner wisdom complements the acquisition of external knowledge, fostering a well-rounded and balanced approach to learning.
- Adaptive Learning Resilience: Acknowledging the dynamic nature of both the Holobiont and Dao, the Liquid Learner theory promotes adaptive learning resilience. Learners are encouraged to adapt to changing circumstances, embrace the unexpected, and find opportunities for growth in challenges. The learning journey is viewed as a continual process of adaptation and evolution.
- Holistic Well-Being in Learning: Holistic well-being is a central goal of our learning theory. Beyond academic achievement, the theory emphasizes the well-being of the learner as an individual and their contribution to the well-being of the learning community. Learning becomes a transformative journey that enhances not only cognitive abilities but also emotional, ethical, and social dimensions of well-being.
Thus the Liquid (Holobiont) Learner theory envisions a symbiotic relationship between the learner, virtues, cosmic harmony, and the learning ecosystem. It embraces diversity, spontaneity, and adaptability, fostering not just the accumulation of knowledge but the development of virtuous individuals who contribute to the flourishing of the broader learning community and the cosmic order.
Please see also:
I used the chemical compound dimedone, which was purchased 13 years ago, as a starting material for the synthesis of other compounds, but unfortunately, it was contaminated with two other materials. The newly synthesized materials stayed in a liquid state and didn't solidify. Does the age of the starting material affect the physical status of the newly synthesized compounds?
My research is green synthesis of urea nanoparticles and I prepared urea nanoparticles contain solution. I need to characterize my sample, but it is difficult to obtain sufficient amount by powder form. Can I get SEM images from liquid nano urea particles contain sample. Is there available easy method to dry my sample?
Calculating the specific heat of a simple liquid by the number of elastic oscillators.
Calculate the specific heat of a simple liquid using the number of elastic oscillators
Each liquid molecule has an average of 8 elastic oscillators around it, and the specific heat contributed by the elastic energy is 4R。Therefore, near the three phase points, the specific heat at constant pressure of a single atomic liquid is 5.5R, and the specific heat at constant pressure of a diatomic liquid is 6.5R. Low temperature liquids such as Ar, Kr, Xe, O2, N2, F2, etc. conform to this conclusion.
Please read the following link for details
please i would like to collect all the parameters related to the synthesis of nanoparticles using laser ablation, in terms of laser parameters, liquid parameters, environment parameters and if any other parameters
Thanks
I want to digest sumo/His label when the protein in still hang on the Ni column. I wash the undesired proteins as usual, then balance the column with 3ml enzyme digestion buffer, then add sumo enzyme for digesting the fusion protein at 4℃ for 12h in 4ml sumo digesting buffer. Then catch the flow through liquid, take some sample from flow through liquid and beads after elution for SDS-PAGE, the brand explains the label did not have been cut, it is still on the beads entirely.
Hi everyone,
I'm new in ASPEN, my question is related to the possibility of simulating an adsorption column where on one side you have a stream of pure hydrogen and on the other side you have a stream of a liquid metal. The objective of this column is to solubilize the hydrogen in the liquid metal according to Sieverts' law. Then, as an apposite step, I would like to simulate the stripping of the hydrogen solubilized in the liquid metal by using an inert gas.
My question is twofold: 1) is it possible to add a liquid metal with known thermophysical and transport properties in ASPEN? 2) how do I have to implement the Sieverts' law since only Henry'ss law seems to be implemented in ASPEN?
The absorption column is indeed a packed column.
I have used PEG as supposed it would be stable in ph 2 and ph 7 and by shifting their peak can understand ho much is the retention time, but have nt yet get any good results
For anyone that has used a Talos S/TEM with a large liquid nitrogen dewar attached, how do you fill your tank? The inside of the microscope is very cramped, and it is awkward to pour the liquid nitrogen from a transfer vessel into the dewar. We have been looking at purchasing a 10-20 L dewar fitted with a withdrawal device and hose attachment so we can transfer the liquid nitrogen directly from a large dewar into the microscope's dewar. I'm curious if there are any other solutions I'm overlooking.
If I add a dye to a liquid, and this liquid is subjected to a high temperature may reach 110 oC does this temperature will affect the dye colour or not
When I conduce an esterification reaction using oleic acid P.A, occurs a variation in color of final product generated (liquid). Is this color due occurence of some concurrent reactions generating other products in medium? Which are the majority products in this reaction that causes this phenomenon?
All the methods to synthesize silver graphene quantum dots that i can find are in liquid form. I do not have the facility of testing liquid samples for FTIR, XRD, SEM etc as the testing centre requires solid or dry samples only.
Can anyone please suggest me if i can get the nanocomposite by centrifuging the liquid samples? Or any other method entirely to synthesize the nanocomposite in the solid form?
steps of preparation a semen cryoprotectant.
We have liquid waste from the by-product of molasses fermentation into glutamic acid. The problem is how to concentrate on this waste. If you have a suitable membrane suggestion, please let me know.
I will use hydroiodic acid to wet etch tin dioxide, but how to treat hydroiodic acid waste liquid is a problem.
J.R. Grace, T. Wairegi, T.H. Nguyen, Shapes and velocities of single drops and bubbles moving freely through immiscible liquids, Trans. IChemE. 54 (3) (1976) 167–173.
Grace, J.R. (1973) Shapes and Velocities of Bubbles Rising in Infinite Liquids. Transactions of the Institution of Chemical Engineers, 51, 116-120.
Solute is toxic liquid so i have to use in microliter.
Which part of the Earth receives the least sun's rays and what part of the water cycle is when liquid water moves through the soil to become groundwater?
Which shape of water surface will give the highest rate of evaporation and why does water Vapour takes up more space than the same amount of liquid?
When the rate of evaporation equals the rate of condensation does the partial pressure of a volatile liquid in a sealed container change?
Why do liquids with fewer polar bonds evaporate faster and rate of evaporation of a liquid related to the intermolecular forces acting on it?
I extraction RNA from Physcomitrella patens (Moss) Following the protocol of RNAiso Plus. Firstly using a tissue lyser and added 1 ml RNAiso plus and vortex. next step Add the chloroform after centrifuge I separated the top liquid layer but the problem is yellow color liquid. I could not avoid the yellow color at ned of the extraction process after the precipitated pellet is brown.
Does the higher boiling liquid have stronger intermolecular forces than the lower boiling liquid and high viscosity mean strong intermolecular forces?
Why solubility of solid in liquid increases with increasing temperature and effect of temperature in the change from solid to liquid?
While in piezoelectric ceramics, a liquid phase can be observed easily with a conventional solid method preparation, however there seems to be no research to figure out whether the liquid phase can affect the mechanical quality factor (Qm) of ceramics.
How can we increase the solubility of a solid in a liquid and hHow does solubility of solid changes with temperature?
What increases the rate of dissolving a solid and factors that affect the rate of solubility of a solid into a liquid agitation temperature and surface area?
Hi,
I’ve recently started having issues with a Zeiss axio cam HRm camera. The camera’s serial number and other informations are available below.
The problem is that something is obscuring the left part of the camera’s field of vision (see attached picture taken by the camera). This issue is specific to the camera, not the microscope, because it is still present even when detached from the microscope. When I look inside the camera I do not see anything visibly blocking the sensor. The camera always stays connected to the microscope from the left side so no dust/liquid/condensation should have been able to gather on the sensor.
Does anyone have any idea what could be responsible for this issue, and how to solve it?
Camera information:
axio cam HRM
60 N-C 1" 1,0x
Serial number: 1 02 02 1247 r2.0
axiocam b/w 12v DC
000000-0445-553 0.7A
ITE 93JA
Why do liquids have a fixed volume but not a fixed shape and what happens to the density and temperature of a gas as it expands?
I am working on Dead gene. my work is related to liquid liquid phase separation for cold shock response and i want to design primer for this amd i have not eniugh information how to select DNA sequence. and than choose c-terminao region to knockout mutants . i want some help in this regard.
One could argue that when R = KLaf ( Cs - C), the fugacity (the maximum transferable rate) has been reached and so the OTR or Rd is zero at that gas flow rate representing the energy input. However, this is a state that could not exist at that given gas flow rate, because OTR can never be zero in a respiring system. The case in which R = KLaf (Cs - C) is not a stable situation (i.e., not a steady state and C ≠ CR), which implies that the actual transfer rate, as opposed to total potential oxygen transfer rate, to the liquid is less than R and the DO concentration is decreasing because the consumption rate has exceeded the transfer rate so that the system is now outgassing oxygen. However, this does not mean Rd is negative. That is, the actual transfer rate, the net oxygen transfer rate given by Eq. (10a), i.e., OTR = KLaf (Cs – C) – r, is not KLaf (Cs - C). To maintain a given DO set point, the air flow rate (AFR) would have to be increased to a new KLa value such that ultimately R equals the actual transfer rate to the liquid. A change in air flow rate would result in a different transfer efficiency, at least in a fine bubble system where an increase in air flow decreases efficiency, and so the true C* also would be nominally different (higher, actually, due to lower gas side depletion), but eventually a steady state would be reached in which the oxygen consumption (R) would exactly match the oxygen transfer rate. For any further increase in the organic loading rate (OLR) and if C > 0, the system can respond by lowering C so that the driving force increases, giving more impetus to transfer. However, when the consumption rate exceeds the oxygen transfer rate, CR approaches C, which itself is ever-decreasing, such that dC/dt is a decreasing function of the consumption rate, i.e., dC/dt < 0. Therefore, the system is no longer in a steady state in such an event. Eventually a point is reached at which C becomes vanishingly small such that even the maximum fugacity is not enough to satisfy, and so the only remedy is to increase the gas flow rate again to match the demand. The conclusion of this exercise is that, for submerged aeration in which the gas loss rate from the system is significant, the rate of transfer under the action of microbial respiration must be given by Eq. (12b) i.e., dC/dt = Kla (Cs – C) – 2Ru, in which both the associated liquid phase oxygen equilibrium concentration (Cs) and the apparent oxygen saturation CR will decrease accordingly (such a phenomenon can be experimentally verified in a converse manner by a reduction in the microbial GDP (the resistance due to biochemical reactions), the net result of a dilution is that both the associated liquid phase oxygen equilibrium concentration Cs and the apparent oxygen saturation CR will increase accordingly. (It is notable that the latter increases faster than the former, so that at R = 0, the rise of CR catches up with the rise of Cs, and so both become one and the same, C*inff).
Mines' paper began on the right track by citing Bartholomew, Albertson and DiGregorio, and some others like Eckenfelder, that there is definitely a relationship between KLa and OUR and so Mines conducted his experiment. Herein lies the difference: Those previous researchers used plant operation data, where the DO is maintained constant. One can only have either constant DO or constant AFR (aeration gas flow rate), but not both. Mines' attempt to verify the dependency of Kla on OUR is premised on constant AFR which is exactly right but he used the wrong equation, resulting in Table 3 and Table 4 that yield the strange result that at steady-state, the OUR is not the same as the OTR. Had he used the right equation, he would have got a consistent result that would support my theory. The consequence of an increase of Ru can only be a reduction of OTR for a constant AFR. It can never by an enhancement! Mines' equation 6, stating that Rd = KLa (Cs- C) - Ru + Ri is therefore insupportable.
His experiment needs to be repeated, but with the following caveat:
equations must be correct, i.e., equation 7 must be written OTR = alpha KLa(beta Cs - C) – Ru resulting in the accumulation term as:
dC/dt = Kla (Cs – C) – 2Ru
OUR of the mixed liquor suspended solids as determined by Method 213B in Standard Methods must be modified to eliminate the shaking effect;
the OTR should be independently measured by the offgas method to compare with the modified Equation 7, since the offgas method is widely considered the best way to determine OTR.
It is important to recognize that the transfer equation given by Equation 1 in Mines' paper, is only valid when R = 0. When R changes, both Cs and OTR will change, even though C changes, (decrease to increase the driving force, or increase if the AFR increases).
When the rate of evaporation equals the rate of condensation does the partial pressure of a volatile liquid in a sealed container change why or why not?
Does stirring increase the rate of solution of a solid in a liquid and how does sugar dissolve faster in water when it is stirred?
What happens to the viscosity of a liquid when temperature increases and why viscosity of gases increases but viscosity of liquid decreases with increase in temperature?
What is the rate of evaporation in dynamic equilibrium and constant temperature at which the vapor pressure of the liquid is equal to the atmospheric pressure?
Hi everyone,
I am currently working on batch experiment to quantify metal adsorption onto different materials. Because the variation of pH after addition of the material is significant and that we don't want to adjust the pH after addition (I am afraid it might impact the surfaces of the material), I wanted to know if a buffer solution can be used to limit the impact of the pH variation.
Also, I noticed there are two ways of doing batch sorption experiments: Lot of researchers varied the initial concentration keeping a fix Liquid to solid ratio to calculate the qe but others varied the liquid mass and the solid mass and maintained the Cini. What do you think are the best approach and what are the pros and cons of each?
Thanks in advance for you help!
Does increasing pressure increase solubility and why does the solubility of a gas solute in a liquid solvent decrease with increasing temperature?
Which condition will increase the evaporation of water and liquids have high viscosity and what do we call the ability water has to flow upward against the force of gravity?
Does surface tension depend on the type of liquid and what do stronger intermolecular attractions cause liquids to have?
specially about the gradual freezing do we need a gradual freezing for animal tissue like cell culture in liquid nitrogen ?
The Biuret protein method uses liquid samples. But how can I obtain a liquid protein extract from solid samples? Can you please share with me some methods or references about it?
Hi, I am Yuan and I am new to this experiment. I aim to express a protein by pGEX construct (Ampiciline), I used BL21 to do transformation, and I got some colonies on the plate(LB Amp) but when I grew them in the liquid medium. They didn't grow at all.
I am very sure I have picked up the colonies and they were in the medium. I tried a few times of transformation before I got this plate with colonies(Attached). I am very confused about it. Because If they could grow on the plate, why couldn't they grow in the medium which has the same recipe other than It has agar?
I would appreciate it very much if I could get any help. Thank you very much!
In the liquid phase sintering of Tungsten-Iron-Nickel powder compacts, could the presence of carbon (between 0.6% and 1.5%) significantly influence the final microstructure by altering the diffusion and the precipitation of W in the Fe-Ni matrix?
The available materials are autoclavable glass, kork, plastic
First, I mixed Hydroxypropyl Methylcellulose with DI-Water till homogeneous, then I poured KOH solution into the Hydroxypropyl Methylcellulose mixing and heated to 60 - 65 degrees Celcius.
The fatty acid was heated to 65 - 70 degrees Celcius to liquid and poured into the Hydroxypropyl Methylcellulose - KOH mixing (main tank), btw the temperature of the main tank after putting fatty acid increased to 8 - 15 degrees Celcius. Is the increase possible from the fatty acid solution or the reaction of fatty acid and KOH solution? How to control the temperature main tank around 70 - 75 degrees Celcius?
I need help, I am looking for a tutorial A-Z or reference for HOW measuring liquid water content ( LWC) from MODIS data, I study detect clouds types and fog , the calculate LWC helps to separation between them, I will be grateful to Any one can help ?