Plants - Science topic

Hello, we're working on SOD levels in pine trees and the protocol for SOD extraction doesn't work? Maybe someone has any protocols meant for trees (conifers) or any helpful suggestions?

Hello,

do you think that full spectrum LEDs are less useful than red and blue light for growing green, leafy plants (e.g. lettuce, spiderwort, cress etc.)? I have read many articles on this and know the theory. In practice, however, I see many greenhouses, especially in vertical farming, in which full-spectrum LEDs (cool white) are used. So who is right?

Thank you!

Yellowish flowers, round green fruit

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Dear all

need help to identify the plant

thanks

Dear All

Please help me to identify the plant/

Thanks in advance 

Rishad

I found this lonely flower in almost all the site where I found the red clover. Is it the partner of the red clover or something like that cause i saw it every time? I didn´t find information but I think it is a Taraxacum officinale? If someone has information, let me know.

With a rapidly growing global population, concerns and questions about the ability to feed the ever-increasing number of people living on planet Earth have been around for many years, but still remain. With a growing population, the scale of civilisation's processing of environmental matter, the scale of industrialisation, the consumption of renewable and increasingly also non-renewable raw materials, the increasing scale of waste generation, environmental pollution, greenhouse gas emissions, etc., the possibilities of feeding the world's population in the future will diminish rather than increase. In addition, the negative effects of the development of civilisation, including above all the accelerating process of global warming, may develop at an even faster rate. Therefore, it is necessary to urgently shift the development of civilisation towards sustainability, to significantly increase the scale of implementation of sustainable development goals, to carry out a green transformation of the economy, including a green transformation of agriculture, and to create new forms of sustainable organic farming, which will be more resistant to negative biotic and abiotic external factors, including, above all, limited resources of agricultural land and the successively increasing negative impact of the progressing process of global warming, including increasingly frequent periods of drought, soil barrenness, plagues of pests and various diseases affecting agricultural crops, etc., which will have a negative impact on the development of civilisation.

Therefore, combining the seemingly contradictory goals of increasing the production of food for people with a reduction in the agricultural areas on which agriculture is practised according to the traditional production formula, stopping deforestation turned into aforestation, increasing the production of crops for human rather than livestock consumption, significantly reducing greenhouse gas emissions, increasing the scale of pro-environmental agriculture, etc., it is precisely the development of vertical farming that can help in this process of green transformation of agriculture.

Vertical farming, also referred to by such terms as vertical farming, vertical farms, consists of growing crops in a vertical formula, in which individual plants are placed one above the other on multi-level platforms. This results in a more efficient use of space, practically multiplying the cultivation area. In addition, vertical cultivation can be established and cultivated anywhere, even in the centre of a large city. Vertical cultivation can be established both in specially designed halls and by adapting existing buildings. Crops such as lettuce, strawberries, herbs and mushrooms, for example, are grown in vertical racks, towers with troughs or purpose-built systems with continuous irrigation and additional artificial lighting.

In view of the above, vertical farming is becoming an excellent alternative to traditional agriculture. The vertical positioning of plants allows a very good use of space, as a result of which significant yields can be obtained from a small area. Vertical farming uses up to 10 times less water compared to traditional agriculture. In addition, vertical farming does not use pesticides and other chemical plant protection products, as the plants are grown in closed, air-conditioned rooms with artificial lighting.

These can even be created in multi-storey buildings in cities or in halls located on the outskirts of cities. This also saves on transport and increases the food self-sufficiency of cities. The problem of droughts occurring more and more frequently in areas of fields cultivated under traditional agriculture does not apply to vertical farming. Therefore, vertical agriculture can be an excellent complement and can also partially replace traditional agriculture in the context of the progressive process of global warming. Therefore, in the context of a progressive global warming process, vertical farming can be an excellent complement to traditionally practised sustainable organic farming. In addition, vertical farming, due to the absence of pesticides and other chemical plant protection products, can also be carried out according to the basic principles that apply to organic farming.

In view of the above, I address the following question to the esteemed community of scientists and researchers:

And what is your opinion on this?

What is your opinion on this subject?

Please respond,

I invite you all to discuss,

Thank you very much,

Hoping to hear your opinions, to know your personal opinion, to have an honest approach to discussing scientific issues and not ChatGPT-generated ready-made answers, I deliberately used the phrase "in your opinion" in the question.

The above text is entirely my own work written by me on the basis of my research.

I have not used other sources or automatic text generation systems such as ChatGPT in writing this text.

Copyright by Dariusz Prokopowicz

Best wishes,

Dariusz Prokopowicz

this plat was found in palm orchards with aromatic odeur

Leaves of several plant species lost chlorophyll after infestation by Spanish slugs (Arion vulgaris). Is it specific slug toxin or some specific virus vectored by the slug?

Dear researchgate members,

I recently made two attempts to grow the aquarium plant Cryptocoryne wendtii emersed, i.e., outside of water. Unfortunately, both attempts failed, and I am unsure of what went wrong.

In the first attempt, I heated soil in the oven and shaped it into a cube. I then placed the aquarium plant into this cube. In the second attempt, I used rock wool instead. In both cases, I lightly moistened the soil and rock wool with aquarium water. Subsequently, I placed them in plastic bags and provided CO2 by exhaling into the bags through a straw. The bags were sealed with rubber bands and positioned under an LED strip light. The distance between the light and the plants was approximately 10 cm, ensuring that the light intensity was not harmful.

After one week, I exchanged the air inside the bags and provided more CO2 by breathing into them again. Unfortunately, after two weeks, I couldn't observe any positive results. Almost all the plants in both the soil and rock wool died. There was no growth observed, neither in the plants themselves nor in the roots.

I am very confused and frustrated, as I don't understand where the mistake lies. Do you have any ideas or advice on what I might have done wrong? Are there specific conditions that I should consider to achieve successful emersed cultivation of Cryptocoryne wendtii?

I would greatly appreciate your help and support! Thank you!

I am curious about the technology behind plant identifier apps. How are they coded? Is there already an app that can differentiate all the species in one frame? For example, it can id each species in a bouquet of flowers?

Hello everyone,

I am sorry in advance for my english, it is not my mother tongue.

I am a student who's currently working on a protoplast regeneration project. Unfortunately there's a problem with the medium I'm using.

I am trying to develop a protocol for protoplast regeneration from a plant (can't say which one). I've started since 4 weeks and last week was the first time of trying a new medium.

I used the next supplements:

- MS medium with Modified vitamins (100%-50%)

- 1% Sucrose

- 9% Mannitol

And after autoclaving this I added:

- 10mg/L Zeatin

- 10mg/L 2,4D- or 50mg/L NAA

I used different concentrations of MS medium with modified vitamins.

I had 9 different media:

MS 100% with standard sugar (as stated above) (with zeatin & NAA) (did not crystalize, but had crystals in medium)

MS 75% with standard sugar (as stated above) (with zeatin & NAA) (crystalized)

MS 50% with standard sugar (as stated above) (with zeatin & NAA) (crystalized)

MS 100% with standard sugar (as stated above) (with zeatin & 2,4D-) (did not crystalize, but had crystals in medium)

MS 75% with standard sugar (as stated above) (with zeatin & 2,4D-) (crystalized)

MS 50% with standard sugar (as stated above) (with zeatin & 2,4D-) (crystalized)

MS 100% with standard sugar (as stated above) (with zeatin & 2,4D- and active charcoal (0,02%)) (did not crystalize, also no crystals in medium)

MS 75% with standard sugar (as stated above) (with zeatin & 2,4D- and active charcoal (0,02%)) (did not crystalize, also no crystals in medium)

MS 50% with standard sugar (as stated above) (with zeatin & 2,4D- and active charcoal (0,02%)) (did not crystalize, also no crystals in medium)

I used 300 ul protoplast suspension with 2,5ml medium.

I will try to use 5 ml next time but I don't know where the crystalisation is coming from. Does anyone have an idea?

If I have a certain compound and I want to see, how is it metabolised in a plant, what are my options?

Are there other options besides radiolabelled compounds? Are there commercial options to purchase radiolabelled compounds? Would you know about somebody, who would be willing to prepare radiolabelled compounds?

This is one of our works based on analysing the viability of ammonia recovery and removal from manures extracted from dairy farm. These kinds of approaches could be sustainable solutions for mitigating air pollution as well as could be utilised into sustainable energy productions.

Can anyone identifiy this plant? It's a Tilia europaea L.?

In Bangladesh, we want to use liquid form of cow-dung as a fertilizer, which is found from bio-gas plant as a byproduct. But by testing, we find that it has excess of chromium which is very harmful to human health. So we need to remove it. So, how can we do this?

When I feed a particular substance to pea plant, I see conversion to unknown compound.

I don't know, what is the product, I don't know (obviously), what is the reaction and I do not know required conditions (like cofactor(s) etc.).

I intend to identify the product, as that is the easiest thing to do right now, I guess. But I'm afraid that won't help me much, as I won't be able to specify the conditions.

Because any in vitro measurement/detection is ruled out because of the lack of knowledge about the reaction conditions, it seems to me like the only option is to go in planta. What I could come up with is either random mutagenesis or finding two genotypes with different activities and then trying to cross them.

However, for that I would need to have either reliable sensor to monitor amount of my compound in planta, or feed it to the plant and then isolate and measure on HPLC/MS (not very high-throughput).

I was also thinking about utilizing cDNA library, but so far, I did not come up with simple way to do so.

Hi, I have problem to sterilize freshwater plants (Anubias, Bucephalandra). I used chlorine salts and ethanol. I got contaminations. I think AgNO3 could works. Do you have any other protocols for sterilization?

Thanks for all responses.

Bohuš

Hi,

I am working on real-time monitoring of plant moisture content and adjusting (optimizing) the irrigation in hydroponic farms with the help of spectroscopy in order to avoid water stress.

For the reference moisture content of plant/leaf, is there any standard method that I could follow? If yes, what is the number or name of the method?

I looked into the literature and I ended up with two methods that were mostly used by the other but I couldn't find the motivation for choosing one over the other.

1) Collecting leaves, and oven drying for several hours (different papers suggested different hours but mostly 48-72h), at 105-degree celsius (while some papers used lower temperature)

Mc = ((Wc - Wd)/Wc )*100

where Mc indicates the percentage of moisture, while Wc and Wd represent the initial weight and the final constant weight of leaves, respectively.

2) Collecting leaves, and hydrating them to get turgid weight. Oven dry at 80-degree C for 24h

RWC (%) = [(W-DW) / (TW-DW)] x 100,

Where W is fresh weight, TW is turgid weight and DW is the dry weight.

Thank you in advance for your help.

Good day,

i have a general question about tissue culture.

I have found the following recipe for Epipremnum Aureum "Marble Queen":

Leaf Explant: MS Medium + 4.54 µM TDZ + 1.07 µM NAA (Thidiazuron in Micropropagation of Aroid Plants by Chen and Wei (2018), p. 105, DOI: 10.1007/978-981-10-8004-3_4)

Specifically, I have the following questions.

1) Do i only need to autoclave the agar with distilled water (I use a pressure cooker for this) and when the agar has cooled down a bit just add the MS, TDZ and NAA and mix it or do i need to autoclave the MS as well?

2) Will the TDZ dissolve in the agar water at all and how hot can the agar water be to add the MS, TDZ and NAA?

3) Is it even necessary to autoclave the water incl. agar (in the pressure cooker) if I clean all the jars with NaClO (sodium hypochlorite)?

Thank you in advance!

...

From the perspective of Plant Population Ecology, what are the causes of drug-resistant plants and how to effectively manage drug-resistant plant species.

We can measure exchangeable K, Ca using flame photometer. Is it possible to analyse these ion using spectral analysis

Latest CEPCI index I could get is 2015, anyone knows how and where to acqueri the information on 2016 CEPCI value?

I would say most invasive alien plants are synanthropic species in that they were introduced intentionally for horticulture or other cultivation purposes, or they were unintentionally introduced but are 'weeds' that thrive in disturbed areas and associated with cultivation at some point. I'm struggling to think of good examples of non-synanthropic invasive plants, but I can think of animal examples.

Among the cultivated primroses, I observed a plant with 5 stigmas and styles. I could not find a report on this. Does anyone know the reason for this feature? Is there a report on this?

I am not ruling out that answers are already in some published papers.

Could you please give more references?

Hello all, I measured XRD in sieved dried surface soil samples but one sample has broad hump around 20 degrees. What do you think about that? Is dried plants shows this kind of pattern? Because after the drying, sample looks like including plenty of dried little particles of plant maybe is that a reason of that pattern?

How to compare images from thermal sensors with different sensitivity and thermal accuracy specifications? What is the significant impact of these specifications in interpreting plant images?

Mario

Hi, I want to know which method is most accurate in providing estimates of chlorophyll content.

Taxonomy (a branch of biology), for example, is a basic science discipline that primarily deals with the identification, classification, and nomenclature of plants. It also contributes to biodiversity and conservation. However, it has been largely overlooked in recent times due to the fact that it has been unable to grow broader impacts or, maybe, due to other emerging applied fields. This question is being posed to discuss the broader impacts of basic sciences in general, and taxonomy in particular.

Hi

I’m looking for free online conferences on plant science / food and nutrition / agriculture / horticulture etc.

Hi,

I’m a PhD student. I’m looking for a short term scholarship for PhD students.

The aim of it is to prepare a common publication (selenium, zinc, bioactive compounds in plants).

Dear Researchers,

Please share methodologies to quantify the hypothesis that the "reduced evaporation" and "increased transpiration" from tree cover area (forested region) results in increased water conservation.

Thank you!

we are working on plant remains from a neolithic site and we are wondering if there is a good and helpful Atlas to compare and identify the plant remains, such as seeds, forks, spikelets, etc. our work is just to prepare a primary report to work with a professional Archaeobotanist.

thank you.

Suppose we have the data for the number of flowers, fruits, branches, leaves (R1=3; R2=3; R3=4; Mean 3.33).

I need to know the name of this plant. I appreciate the help.

I expend almost 2 months trying to find the name of this tree, if you know it I will appreciate the help.

Hi

Would you please tell me what plant it is?

Hello,

I work for an organization that tests various products on plants: substrate, natural fertilizer, biostimulant, etc.

With each new project, we ask ourselves which plant are we going to use, what parameter are we going to measure, is it relevant or not for this plant, for these products, etc.

So, I was wondering if there is a guide to research plants and their optimal use?

For example:

-Test: fertilizer for fruit plants.

-Plants: tomatoes and cucumber.

-Measure: number of flowers (p.x how to do it) and weigh the fruits after x days.

Thank you

Hello everyone,

We are planning to study how plant growth promoting bacteria are affecting various plants (eg. wheat, barley, etc.). We would like to coat the seeds with bacteria. We prefer film coating. I can't find any proportions of seed, inoculum, carrier materials and sticking agents.

Could you please share the recipe you are using for your research? We prefer using cellulose or lime as a carrier material.

Thank you in advance.

Hello to everyone! I am trying to multiplicate a specific variety of Kiwi with tissue culture. I managed to multiplicate some. Some of them came from callus and some were produced directly from the starting material. What I would like to ask is, do the plants that come from callus differ genetically from the mother plant?

Hi

I'm researching about porphyrin syntheizing pathways protein and genes...

I need to check heme oxydase activity so need to extract biliverdin IXa(BV), the product of heme oxidation, from plants.

I'll procedure HPLC analyzing but only found extract BV from E. coli, salmon and egg shall not a plants leaf tissue.

So, is there anybody solate biliverdin IXα from plants for HPLC analyze?

Thanks for reading.

Always be happy.

감사합니다.

Is it appropriate to name organisms after people? Names convey messages and reflect attitudes! Is it ethically appropriate to dedicate a plant or animal to a person for whatever reasons? Sandra Knapp, Maria S. Vorontsova, and Nicholas J. Turland refer to this as "symbolic ownership" in "A Comment on Gillman & Wright (2020)" in Taxon https://doi.org/10.1002/tax.12411

BIOPAT e.V., for example, offers to name new species at wish for a donation of at least 2600 Euros (who do these organisms 'belong to'?). One can dedicate a scholarly work, as a book or journal article, to a person – but a living organism? Wouldn't it be preferable to adhere to the common practice of allocating descriptive names and to ban anthropocentric patronymic names and 'graveyard taxonomy' from biological nomenclature via the 'Code'!

I'm doing a research project where we are testing different methods of fluorescent live/dead stains and we need to kill some strawberry and potato roots so we can stain them. The only method we know will work well is boiling them in 70-80 degree water, have any other ideas?

thanks!

It is a medicinal leafy vegetable plant

Demographers estimate that by 2050, the number of people on Earth will reach 10 billion. With such a number of people, the agricultural economy, logistics of food supplies and people's eating habits will have to change. It is likely that economics will force these processes, which will result in the transition of the majority of humanity to nutrition mainly based on vegetable and vegetarian diets. Meat production is many times more expensive than the production of cereals, fruits and vegetables. In addition, according to scientific research and the theory of futurologists, the production of traditional meat, e.g. pork and beef, may be replaced by the production of protein from insect breeding. Research shows that there are more proteins in the bodies of insects than in traditional meat dishes. In addition, the logistics of food supplies, agri-food products will have to improve. Systems for matching agricultural and reptile production to the current needs of the industry and the nutritional needs of people will be improved so as to reduce the scale of food wastage. The biggest threat to the implementation of this plan may be unexpected atmospheric phenomena, natural disasters, droughts, hurricanes, tropical heat in the areas in which agricultural crops have been cultivated so far. In addition, industrial exploitation of arable land and climate change causes soil depletion and the disintegration of areas suitable for agricultural production. Therefore, it will be necessary to continue the technological progress in the production of crops, in biotechnology, in the creation of new plant varieties resistant to pests and adverse climatic changes.

Please, answer, comments. I invite you to the discussion.

Hi everyone,

are there methods for the RNA extraction from mosses spores?

Could a method used for RNA extraction from pollen be suitable for mosses spores?

Thanks

This plant grow in wild habitat and flowers in July and August.

How do we grow plants in marginal environments without encouraging invasiveness?

The yield of plants is found to be increased with the conservation of perennial to annual plants what are the processes, advantages, and disadvantages?

I am researching UKF, and I would like to know where can I find performance measures of this. The system is a Chemical Process Plant.

What are the precautionary measure that we can adopt to prevent the sprouting while drying the plant sample for herbarium?

Please suggest

So I understand that essential oils are formed by steam distillation, and contain volatile, usually aromatic, compounds.

Extracts are formed by immersion of plant matter in a solvent, and are usually reported to contain compounds such as polyphenols, anthraquinones, flavonoids and the like.

Practically every report I have read does not mention whether obtained extracts contain the volatile compounds found in essential oils though. Will there be volatile essential oil compounds in extracts, or can they ONLY be taken out of plant matter by steam distillation?

I know, the question is a bit simplistic. It is also not only about the plants, but more about the photosynthetic organisms ... However, in this time of global biodiversity crisis, we are constantly confronted with prioritizing. Recently I read somewhere that in the Arctic, the photosynthetic algae should be taken care of and not the polar bears. Also in temperate regions, where the habitats are under enormous pressure, shouldn't we pay the greatest attention to the producers/plants? On the local level (local administration, journalists) I am constantly confronted with the question: who do we save first? With this somewhat provocative question, I simply wanted to know/hear what researchers from various fields will say about it (or against it).

Are pathogenesis-related proteins and antimicrobial peptides encoded by plant resistance genes (R genes)?

I'm unsure if the genes for these proteins/peptides are types of R genes?

My research includes monitoring canopy temperature over time with a thermal FLIR camera. I am looking for a code/program that will help me analyze the videos easily and automatically. For my analysis, I am looking for a code that can separate plants and background and that distinguish between the different plants. I then need the average temperature from each plant in every frame.

The file we get from the thermal imaging is a 'seq' file.

I attached a RGB picture of a tray in my experiment.

Thank you in advance for your help.

How does MAPK signalling work in plants in response to pathogen infection?

This is a climbing shrub grown in houses for the fragrant flowers in Salem, South India. I request the expert members for the species id of this Jasminum.

Thank you.

As the land availability for conventional farming is decreasing, what percentage of worldwide farming is being done through soilless route (including hydroponics, aeroponics, aquaponics)?

Any literature highlighting the same will be helpful please.

I am attaching the photos of a Rubiaceae member for identification which I have taken from Oddanchatram, Dindigul district of Tamil Nadu, India.

I request the specialists to identify the plant.

Thank you.

I have some plant samples and I want to check the antiviral activity and Antibacterial Activity. and I want to check it by using bioinformatics.

So I need Suggestions regarding which bioinformatics parameter or software is available that I can use for check by antiviral and Antibacterial activity.

#bioinformatics #antiviral #antibacterial #plants

How many new plant species have been discovered in Hoang Lien Son range since 2000?

Dear all taxonomists

Do you think that the problem which we had with Zootaxa in this year is an accidental or we should expect similar problems in the future? Not only for Zootaxa but also with other taxonomic journals. Do you think that we can do something with it? Any strategy or ideas? Do we have this problem only in zoology or also in botany?

Do you use image analysis to make measurements in your experiments?

Would a monitoring camera help prevent failed experiments?

Do you need lots of pictures to document experiment progress?

My hope with this discussion is to identify problems that I could solve. I am developing camera hardware and software tools and want to make them as useful as possible to researchers.

Please help me to identify this fern species. Thank you in advance.

In your opinion, what plant breeding plant can be important and useful in the future?

I have seen farmers are using Bentazol for broadleaved weed control on soya bean fields. Soya bean byitself is also a broad leaved plant. I want to know more about this.

The Capparis scabrida species, Family of the (Capparaceae), is known as dog sapote, it is one of the species with the greatest adaptability to desert ecosystems, it belongs to the flora of the dry forests of Northern Peru, and Central and South from Ecuador, between 0-2,500 m of altitude. It is a species that never loses its leaves, always turn green during its life cycle and it does not have seasonal precipitation, it has to wait for the rain every year. My question is why it forms many rings of growth if it does not present seasonal precipitation and never loses its leaves. That is an image of a cross section so you can see the great presence of growth rings, It was collected in the South Region of Ecuador (Loja-Catamayo,Ecuador 2020).