Science topic
Green IT - Science topic
Explore the latest questions and answers in Green IT, and find Green IT experts.
Questions related to Green IT
what are the strategies green procurement?
how can the be measured in relation to organizational performance
Will technological advancements be able to solve the climate change problem and why is green information technology important for climate change issues?
How does green technology affect sustainable economic growth and development and difference between eco-friendly, going green, and sustainability?
With progressive increase in A/C machine increasing green house gases ( CFCs) and vice versa . It is a cyclic problem through out the globe . Please suggest your views.
How green economy can lead to low-carbon economy and difference between sustainable development and green development?
Is the PARIS AGREEMENT A REAL DEAL FOR CLIMATE CHANGE? This was the big question raised in my postgraduate open forum on the New GREEN DEAL. There were no clear-cut answers to that HOLY GRAIL question. is the current progress showing good progress or is it just another KYOTOL PROTOCOL backwater?
I'm synthesizing a substituted phthalocyanine from phthalonitrile in n-pentanol solvent using DBU as a base and a metal salt but the colour of reaction mass is not turning blue/green after refluxing as reported in the literature. When I try this reaction in a small scale (50 mg), I get a blue/green colour. What went wrong? Now I am only getting a brown colour formation which may due to polymerization or triazine formation of the substrate. What precautions should I take to always get the desired phthalocyanine product?
Is there significant difference between these other element present which highlighted as black? This is from Scanning Electron Microscope-EDS
How to build a sustainable data center based on Big Data Analytics, AI, BI and other Industry 4.0/5.0 technologies and powered by renewable and carbon-free energy sources?
If a Big Data Analytics data center is equipped with advanced generative artificial intelligence technology and is powered by renewable and carbon-free energy sources, can it be referred to as sustainable, pro-climate, pro-environment, green, etc.?
Advanced analytical systems, including complex forecasting models that enable multi-criteria, highly sophisticated, big data and information processing-based forecasts of the development of multi-faceted climatic, natural, social, economic and other processes are increasingly based on new Industry 4.0/5.0 technologies, including Big Data Analytics and machine learning, deep learning and generative artificial intelligence. The use of generative artificial intelligence technologies enables the application of complex data processing algorithms according to precisely defined assumptions and human-defined factors. The use of computerized, integrated business intelligence information systems allows real-time analysis on the basis of continuously updated data provided and the generation of reports, reports, expert opinions in accordance with the defined formulas for such studies. The use of digital twin technology allows computers to build simulations of complex, multi-faceted, prognosticated processes in accordance with defined scenarios of the potential possibility of these processes occurring in the future. In this regard, it is also important to determine the probability of occurrence in the future of several different defined and characterized scenarios of developments, specific processes, phenomena, etc. In this regard, Business Intelligence analytics should also make it possible to precisely determine the level of probability of the occurrence of a certain phenomenon, the operation of a process, the appearance of described effects, including those classified as opportunities and threats to the future development of the situation. Besides, Business Intelligence analytics should enable precise quantitative estimation of the scale of influence of positive and negative effects of the operation of certain processes, as well as factors acting on these processes and determinants conditioning the realization of certain scenarios of situation development. Cloud computing makes it possible, on the one hand, to update the database with new data and information from various institutions, think tanks, research institutes, companies and enterprises operating within a selected sector or industry of the economy, and, on the other hand, to enable simultaneous use of a database updated in this way by many beneficiaries, many business entities and/or, for example, also by many Internet users in a situation where the said database would be made available on the Internet. In a situation where Internet of Things technology is applied, it would be possible to access the said database from the level of various types of devices equipped with Internet access. The application of Blockchain technology makes it possible to increase the scale of cybersecurity of the transfer of data sent to the database and Big Data information as part of the updating of the collected data and as part of the use of the analytical system thus built by external entities. The use of machine learning and/or deep learning technologies in conjunction with artificial neural networks makes it possible to train an AI-based system to perform multi-criteria analysis, build multi-criteria simulation models, etc. in the way a human would. In order for such complex analytical systems that process large amounts of data and information to work efficiently it is a good solution to use state-of-the-art super quantum computers characterized by high computing power to process huge amounts of data in a short time. A center for multi-criteria analysis of large data sets built in this way can occupy quite a large floor space equipped with many servers. Due to the necessary cooling and ventilation system and security considerations, this kind of server room can be built underground. while due to the large amounts of electricity absorbed by this kind of big data analytics center, it is a good solution to build a power plant nearby to supply power to the said data center. If this kind of data analytics center is to be described as sustainable, in line with the trends of sustainable development and green transformation of the economy, so the power plant powering the data analytics center should generate electricity from renewable energy sources, e.g. from photovoltaic panels, windmills and/or other renewable and emission-free energy sources of such a situation, i.e., when a data analytics center that processes multi-criteria Big Data and Big Data Analytics information is powered by renewable and emission-free energy sources then it can be described as sustainable, pro-climate, pro-environment, green, etc. Besides, when the Big Data Analytics analytics center is equipped with advanced generative artificial intelligence technology and is powered by renewable and emission-free energy sources then the AI technology used can also be described as sustainable, pro-climate, pro-environment, green, etc. On the other hand, the Big Data Analytics center can be used to conduct multi-criteria analysis and build multi-faceted simulations of complex climatic, natural, economic, social processes, etc. with the aim of, for example. to develop scenarios of future development of processes observed up to now, to create simulations of continuation in the future of diagnosed historical trends, to develop different variants of scenarios of situation development according to the occurrence of certain determinants, to determine the probability of occurrence of said determinants, to estimate the scale of influence of external factors, the scale of potential materialization of certain categories of risk, the possibility of the occurrence of certain opportunities and threats, estimation of the level of probability of materialization of the various variants of scenarios, in which the potential continuation of the diagnosed trends was characterized for the processes under study, including the processes of sustainable development, green transformation of the economy, implementation of sustainable development goals, etc. Accordingly, the data analytical center built in this way can, on the one hand, be described as sustainable, since it is powered by renewable and emission-free energy sources. In addition to this, the data analytical center can also be helpful in building simulations of complex multi-criteria processes, including the continuation of certain trends of determinants influencing the said processes and the factors co-creating them, which concern the potential development of sustainable processes, e.g. economic, i.e. concerning sustainable economic development. Therefore, the data analytical center built in this way can be helpful, for example, in developing a complex, multifactor simulation of the progressive global warming process in subsequent years, the occurrence in the future of the negative effects of the deepening scale of climate change, the negative impact of these processes on the economy, but also to forecast and develop simulations of the future process of carrying out a pro-environmental and pro-climate transformation of the classic growth, brown, linear economy of excess to a sustainable, green, zero-carbon zero-growth and closed-loop economy. So, the sustainable data analytical center built in this way will be able to be defined as sustainable due to the supply of renewable and zero-carbon energy sources, but will also be helpful in developing simulations of future processes of green transformation of the economy carried out according to certain assumptions, defined determinants, estimated probability of occurrence of certain impact factors and conditions, etc. orz estimating costs, gains and losses, opportunities and threats, identifying risk factors, particular categories of risks and estimating the feasibility of the defined scenarios of the green transformation of the economy planned to be implemented. In this way, a sustainable data analytical center can also be of great help in the smooth and rapid implementation of the green transformation of the economy.
Kluczowe kwestie dotyczące problematyki zielonej transformacji gospodarki opisałem w poniższym artykule:
IMPLEMENTATION OF THE PRINCIPLES OF SUSTAINABLE ECONOMY DEVELOPMENT AS A KEY ELEMENT OF THE PRO-ECOLOGICAL TRANSFORMATION OF THE ECONOMY TOWARDS GREEN ECONOMY AND CIRCULAR ECONOMY
Zastosowania technologii Big Data w analizie sentymentu, analityce biznesowej i zarządzaniu ryzykiem opisałem w artykule mego współautorstwa:
APPLICATION OF DATA BASE SYSTEMS BIG DATA AND BUSINESS INTELLIGENCE SOFTWARE IN INTEGRATED RISK MANAGEMENT IN ORGANIZATION
I have described the key issues of opportunities and threats to the development of artificial intelligence technology in my article below:
OPPORTUNITIES AND THREATS TO THE DEVELOPMENT OF ARTIFICIAL INTELLIGENCE APPLICATIONS AND THE NEED FOR NORMATIVE REGULATION OF THIS DEVELOPMENT
In view of the above, I address the following question to the esteemed community of scientists and researchers:
If a Big Data Analytics data center is equipped with advanced generative artificial intelligence technology and is powered by renewable and carbon-free energy sources, can it be described as sustainable, pro-climate, pro-environment, green, etc.?
How to build a sustainable data center based on Big Data Analytics, AI, BI and other Industry 4.0/5.0 technologies and powered by renewable and carbon-free energy sources?
How to build a sustainable data center based on Big Data Analytics, AI, BI and other Industry 4.0/5.0 and RES technologies?
What do you think about this topic?
What is your opinion on this issue?
Please answer,
I invite everyone to join the discussion,
Thank you very much,
Best wishes,
Dariusz Prokopowicz
The above text is entirely my own work written by me on the basis of my research.
In writing this text, I did not use other sources or automatic text generation systems.
Copyright by Dariusz Prokopowicz
I'm having great success using Cell Tracker red with Lactobacillus. Cell Tracker Blue CMHC and Green CM-H2DCFDA aren't working. I'm doing some trouble-shooting but I'd appreciate any insights from people who have tried these dyes on bacteria.
At least in the economic arena, by this time the norm should have been green microeconomics and green macroeconomy since 2012 Rio +20 as the tools to be taught to deal with the environmental crisis as consensus on paradigm change to green market, green growth, and green economies was reached/RIO +20 Conference/UNCSD 2012, but traditional economic thinking and traditional macroeconomic thinking is still the norm, which means that universities knowingly or not are normalizing paradigm shift avoidance, and blocking the growth of knowledge a la Thomas Kuhn; and this raises the question: Paradigm shift avoidance and universities, do they have a duty to science based paradigm evolution of knowledge?
what do you think?
Simply state Yes and give your opinion on why you think Yes or say No, and state your opinion on why you think No.
Why is the Coot real space refine zone indicator green even in regions where the model has no density support? Doesn't this indicator combine both stereochemistry and density parameters?
How should a system be built to verify the level of “greenness” of companies and enterprises in order to effectively systemically limit the development of greenwashing?
How should a system be built to verify the level of “greenness” of companies and enterprises, the level of factually inconsistent portrayal in the media and advertising campaigns carried out by business entities as green, pursuing sustainable development goals, respecting norms and standards for the protection of the climate, biosphere and environment, in order to effectively systemically limit the development of greenwashing?
As the level of pro-climate and pro-environmental awareness of citizens increases, so does the number of companies and enterprises that portray themselves in advertising campaigns as business entities pursuing specific sustainable development goals, in line with the trends of green transformation of the economy. To a large extent, these issues are highly correlated with each other and act in feedbacks to each other. On the one hand, the growing level of pro-climate and pro-environmental awareness of citizens motivates business entities to add to their missions and development strategies the issue of achieving sustainable development goals, undertaking business ventures that are part of the green transformation of the economy. On the other hand, in a situation where more and more companies, enterprises, financial and public institutions are presenting themselves as green business entities presenting in various brand promotion activities and advertising campaigns informing about the company's product and/or service offerings also descriptions and characteristics of green business ventures, presenting the business entity's implementation of certain sustainable development goals and green investment projects undertaken, where they develop their own energy sources based on renewable and emission-free energy sources, building wastewater treatment plants so as not to generate waste that pollutes the environment, developing and improving waste segregation and recycling techniques, carrying out economic projects involving environmental reclamation and restoration in post-industrially degraded areas, reforestation of industrially exploited areas and implementation of other green economic activities that are part of the processes of green transformation of the economy. however, as an institutional system for assessing the level of “greenness” of the aforementioned activities presented as realizing the goals of sustainable development and/or being part of pro-climate, pro-environmental, pro-ecological economic ventures has still not been built, so a significant number of business entities are overemphasizing the issue of presenting themselves in the framework of company brand promotion campaigns as green entities implementing ventures that are part of the green transformation of the economy. In recent years, there has been a strong increase in the number of companies, enterprises and financial institutions that portray themselves as green economic entities pursuing certain goals, including pro-environmental and pro-climate sustainability goals, while the scale of real activities in this regard is negligible or almost nonexistent. In addition, the introduction of mandatory expanded, non-financial ESG reporting motivates business entities to pursue green business ventures, which are often not green in reality. This is because a system of verification of the level of “greenness” of companies and enterprises, the level of factually inconsistent portrayal in the media and advertising campaigns carried out by business entities as green, pursuing sustainable development goals, respecting norms and standards for the protection of the climate, biosphere and environment, has still not been built to effectively systemically reduce the development of greenwashing, i.e. the so-called “eco-cheating”, eco-lying.
I am conducting research on this issue. I have included the conclusions of my research in the following article:
I invite you to discuss this important topic for the future of the planet's biosphere and climate.
In view of the above, I address the following question to the esteemed community of scientists and researchers:
How should a system be built to verify the level of “greenness” of companies and enterprises, the level of factually inconsistent portrayal in the media and advertising campaigns conducted by business entities as green, pursuing the goals of sustainable development, respecting norms and standards for the protection of the climate, biosphere and environment, in order to effectively systemically limit the development of greenwashing?
How should the system of verification of the level of “greenness” of companies and enterprises be built in order to effectively systemically limit the development of greenwashing?
What do you think about this topic?
What is your opinion on this issue?
Please answer,
I invite everyone to join the discussion,
Thank you very much,
Best wishes,
Dariusz Prokopowicz
The above text is entirely my own work written by me on the basis of my research.
In writing this text, I did not use other sources or automatic text generation systems.
Copyright by Dariusz Prokopowicz
I would like to track in real time the growth of bacteria using fluorescent miscoscopy. I remember there was at least one plasmid that could be used: transfected bacteria would emit a red or green fluorescent signal that could be used to track their growth and position.
Alas, I don't remember what was the name of these plasmids and I can't find a reference in the literature.
Does somebody know these kind of plasmids for live tracking of bacteria? Where can I buy them?
Thank you.
When I click on the blue tab "Add text publicly", nothing happens. So I try the upper right tab "Add new"->published research->article, then click "Select and upload file". Nothing happens, so I drag and drop the MS (both as .doc and as .pdf), and the green "+" seems to indicate a successful transfer into the box, but nothing actually gets to the RG page.
I've used this method successfully many times in the past, so I'm not sure what's different now. Thanks in advance for any suggestions.
I'm looking for public databases for green finance indicators, which provide information on sustainable investments, carbon emissions, sustainable development initiatives, and other indicators related to green finance. These are used by green banks, businesses, and governments to assess and track progress in the field of sustainable finance.
What is the transition from industrial economy to green economy and challenges when transitioning to a green economy?
Just as in the case of greenwashing where people can get tricked because the word GREEN sounds good, the same can be said with respect to the current move from linear economic thinking to circular economic thinking where some people may be tricked because the word CIRCULAR sounds good.
But those familiar with science based revolutions a la Thomas Kuhn should be able to spot WHERE THE TRICK IS.
And this raises the academic question, Why the current move from linear economic thinking to circular economic thinking is inconsistent with Thomas Kuhn's paradigm evolution loop?
What do you think?
Can you see the inconsistency?
And hence, can you see the trick?
Anyone interested please contact with me
I could find few red seaweeds and brown seaweeds. Any suggestions or links to the reports are highly appreciated.
What is the preferable option for synthesizing green nanoparticles: using fresh leaves or dried leaves?
Hi, Research gate! I need HELP!
I'm trying to prepare nickel nanoparticles for my devices. I use the commonly used method from the article (DOI: 10.1002/adma.201405391). Briefly, 20 mmol Ni(NO3)2·6H2O were dissolved in 20 mL of deionized (DI) water to obtain a dark green solution. Then, 4 mL NaOH solution (10 mol L−1) was slowly added into the solution while stirring. After being stirred for 20 min, the colloidal precipitation was thoroughly washed with DI water three times and dried at 80 °C overnight under vacuum. The obtained green powder was then calcined at 270 °C for 2 h to obtain a dark-black powder. The NiOx NPs inks were prepared by dispersing the obtained NiOx NPs in DI water/IPA (3/1, v/v) with a concentration of 20 mg mL−1, stirred for 30 min.
However, the nanoparticles coagulate (precipitate). I can't seem to get the suspension.
What are the subtleties of synthesis? What am I missing? What should I pay attention to?
I synthesized graphene oxide during synthesis, and when I added H2O2, the solution turned green. What is the reason for this?
It is said that substances such as malachite green (MG) and crystal violet (CV), which are used as Ramandye, are combined with Gold nanoparticle (AuNP) to measure Raman (SERS).
I'm curious about the principle of how malachite green(MG) and crystal violet(CV) combine to Gold nanoparticle(AuNP).
Thank you!
For a while I am searching on internet for types, material and especially: mesh-sizes of outdoor-proof insect netting for emergence/breeding-experiments. Its to breed a complete community from logs and standing deadwood.
Is someone experienced with this?
Ideally, it would be:
- mesh size 0.5-0.5mm;
- durable (not easy to destory
- black, or dark green/ brown colour
Thanks in advance for the advice!
Dear colleagues
i have synthesized CeO2 using green method and now the Np are not soluble in water how can I make it soluble in water?
Best Regards
Hello,
My E. coli cells express both green fluorescent protein as well as mCherry. So I need a fluorescent stain of color other than green and red fluorescence to enumerate their viability. Please suggest. Thanks in advance.
I have several cell in one picture, all of them contains vesicles. The cells labelled with red marker. the vesicles labelled with green marker. I would like to know each cell how many vesicles located inside. I would like to count all cells which are located in my image at once. How it is possible to do it?
Dear all,
I am wondering if you have ever experienced bleed-through using the 680and 800 infrared dye secondary antibodies from LiCor. I observed a possible bleed through from a highly expressed protein (red channel, 680nm) into the green channel (800 nm). What is your experience? How did you solve it?
Thanks
For the attached model TKN assembly. I digested composted sample in that flask itself. My receiving solution (4%boric acid+Methyl red&bromocrescol green mixed indicator) already in red colour. While I titrate with 0.1 N H2SO4 after digestion I couldnt get green colour in receiving solution but end point in my referred procedure is green to red.
What is green polyol? What is the advantage and disadvantage of green polyol compared to petrochemical based polyol. Why the market focusing on green polyols?
Collections of Green House Gases From Gas Sampling?
How to collect green house gases?
Methods of Collections?
During the Covid-19 pandemic, was there an opportunity to accelerate the processes of green transformation of the economy, and was this opportunity unfortunately not taken advantage of in some countries?
During the Covid-19 pandemic, due to the decline in the economic activity of companies and enterprises in many sectors of the economy, there were opportunities to accelerate the processes of green transformation of the economy, to implement the principles of sustainable economic development, to direct the development of the economy towards the green circular economy model, to achieve the goals of sustainable development, to increase the scale of pro-environmental policies, pro-environmental, pro-climate policy and thus reduce greenhouse gas emissions, reduce the level of environmental pollution, and in the situation of continuation of these processes in the coming years, it is also to increase the scale of the possibility of implementing the scenario of slowing down the progressive process of global warming, to give humanity more time to prepare for the possible subsequent negative effects of progressive climate change, the developing climate crisis. Some countries have taken advantage of these opportunities, but unfortunately only in some countries.
These issues are presented in the article:
The Impact of the SARS-CoV-2 (COVID-19) Coronavirus Pandemic on Ecological Security and the Development of International Environmental Policy
In view of the above, I address the following question to the esteemed community of scientists and researchers:
During the Covid-19 pandemic, was there an opportunity to accelerate the processes of green transformation of the economy, and was this opportunity unfortunately missed in some countries?
During the Covid-19 pandemic, was there an opportunity to accelerate the processes of green transformation of the economy?
What do you think about this topic?
What is your opinion on this issue?
Please answer,
I invite everyone to join the discussion,
Thank you very much,
Best wishes,
Dariusz Prokopowicz
The above text is entirely my own work written by me on the basis of my research.
In writing this text I did not use other sources or automatic text generation systems.
Copyright by Dariusz Prokopowicz
There seems to be a coordinated approach that apparently is centered in and propagating from the EU that seems to have started in 2023, where out of nowhere they are trying to bring back traditional economic thinking into the main stream by defining it circular using academic tunneling and alternative academic facts.
And hence, they perhaps know that a circular definition does not addresses the pollution production problem we have been trying to solve since WCED 1987/Our Common Future through sustainable development means, and later by the UNCSD 2012/The future we want through green economy, green growth and green market thinking to set up the blocks needed to transition to CLEAN MARKETS, all of which are beyond business as usual approaches.
And this raises a basic question: Is the circular traditional market a pollution reduction market?
How can we develop strategies to achieve an effective green economy to achieve real development?
How can artificial intelligence technology help in the development and deployment of innovative renewable and zero-carbon energy sources, i.e. hydrogen power, hydrogen fusion power, spent nuclear fuel power, ...?
In view of the above, with the development of renewable and emission-free energy sources there are many technological and environmental constraints on certain categories of spent materials used in this type of energy. On the one hand, it is necessary for power companies to make investments in electricity transmission and storage networks. On the other hand, economical technologies for the production of low-cost energy storage and recycling, disposal of used batteries and photovoltaic panels, including the recovery of rare metals as part of the aforementioned disposal process, are still to be developed. In addition, the problem of overheating of batteries in electric vehicles and the occurrence of situations of spontaneous combustion of these devices and dangerous, difficult to extinguish fires of the said vehicles are still not fully resolved. If the solution to such problems is mainly a matter of necessary improvements in technology or the creation of new, innovative technology, then arguably generative artificial intelligence technology should come to the rescue in this regard.
I described the key issues of opportunities and threats to the development of artificial intelligence technology in my article below:
OPPORTUNITIES AND THREATS TO THE DEVELOPMENT OF ARTIFICIAL INTELLIGENCE APPLICATIONS AND THE NEED FOR NORMATIVE REGULATION OF THIS DEVELOPMENT
Important aspects of the implementation of the green transformation of the economy, including the development of renewable and zero-carbon energy sources I included in my article below:
IMPLEMENTATION OF THE PRINCIPLES OF SUSTAINABLE ECONOMY DEVELOPMENT AS A KEY ELEMENT OF THE PRO-ECOLOGICAL TRANSFORMATION OF THE ECONOMY TOWARDS GREEN ECONOMY AND CIRCULAR ECONOMY
I invite you to discuss this important topic for the future of the planet's biosphere and climate.
In view of the above, I address the following question to the esteemed community of scientists and researchers:
How can artificial intelligence technology help in the development and deployment of innovative renewable and carbon-free energy sources, i.e. hydrogen power, hydrogen fusion power, spent nuclear fuel power, ...?
How can artificial intelligence technology help in the development and deployment of renewable and emission-free energy sources?
What do you think about this topic?
What is your opinion on this issue?
Please answer,
I invite everyone to join the discussion,
Thank you very much,
Best wishes,
Dariusz Prokopowicz
The above text is entirely my own work written by me on the basis of my research.
In writing this text I did not use other sources or automatic text generation systems.
Copyright by Dariusz Prokopowicz
Please explain the role of green Knowledge management in brief and its influences.
I need CIF file of Berlin Green.
Green house gases and global warming?
Their impact on environment?
Hello, I'm trying to knock down and re-express (both through adenovirus) a protein of interest. My knock down using shRNA targeted to 3' UTR is pretty efficient, but when I try to re-express the protein after knock down, I don't get a full rescue. Specifically, some cells appear to re-express the protein well, but others not so much and still others not at all.
I've attached a couple IF images to show you what I get. Red is antibody against protein of interest and green is the GFP signal from the exogenous protein. As you can see, when I transduce control cells (not shRNA knock down) with the rescue construct, most of the cells appear to get the GFP. When I transduce with shRNA adenovirus prior to rescue, I get a mediocre rescue.
If relevant, this is the rough protocol I follow:
D1: Transduce cells with shRNA adenovirus
D2 morning: change media to complete media
D2 afternoon/evening: wash cells with 1X DPBS, transduce with rescue adenovirus
D3: change media to complete media
D4: harvest/fix/etc
Also, I'm a graduate student still learning so I'd appreciate it a lot if you can explain things in a way that isn't too technical. Thanks for the help in advance.
Edit: Researching further, it became apparent that my protein which forms filament structures cannot form those structures with only the GFP-tagged version. It requires some wild type to be present in order to build upon it. I guess some of my cells got such a good knockdown that it couldn't be rescued by the GFP-tagged construct. I'll leave this question up so that anyone with similar issues can see if this might be the case for them.
Think about it, the WCED 1987 told us among other things that to be environmentally friendly we have to go beyond business as usual using sustainable development means, which by 2012 Rio + 20 the world had agreed that the WIN-WIN economy and environment model was the way to shift to a world under green markets, green growth, and green economies, BUT then soon since 2012 the environmentally sustainability pretending began as instead of green markets the world ended up with green dwarf green markets, dwarf green growth, and dwarf green economies. But now that PRETENDING seems to be coming to an end AS the world, against 100% the Thomas Kuhn's paradigm evolution loop expectation seems ready to go back to square one as in 1987, but now with CIRCULAR traditional economic thinking and academic tunneling. And this raises the question, Does going traditional circular economic thinking means the environmental sustainability pretending is over?
What do you think? Yes, and why you think so? No, and why you think No?
All the F, plants had round seeds with yellow cotyledons. Diagram this cross through the F₂ generation using both the Punnett square and forked-line methods.
Hi, everyone!
I am a graduate student of pharmacology from China. I am trying to measure the plasma NETs level with anti-MPO antibody and Sytox Green, which are available in our lab. Here's how I did it.
Firstly, a high-binding 96-well plate were coated overnight at 4 ℃ with anti-MPO antibody(1 μg/mL, Thermo). The plate was washed 1 time with wash buffer, then blocked with 4% BSA in PBS supplemented with 0.05% Tween-20 for 1.5 hours at room temperature. The plate was washed 3 times again, then incubate with plasm (100 μL) for 2 hours at 37 ℃, 300 rpm. The plate was washed 5 times before incubating for 15 minutes with Sytox Green in dark (100 μL, 1:1000, Thermo). The fluorescence intensity (excitation at 485 nm and emission at 535 nm) was quantified.
But there was no difference in fluorescence intensity between plasma and negative controls. I'm not sure what went wrong. I hope anybody who did it can give me some advice. Thank you so much for your generous help!
Best wished!
Yafei, Fang
Out of nowhere apparently came at the same time in 2023, researchers from different countries, governments from different countries, different international organizations and banks, all are praying in the name of CIRCULAR ECONOMY at the same time, from different angles and levels.
Probably some of them are the same researchers, countries and organizations that in 1987 were praising the SCIENCE BASED call *WCED 1987 Our Common Future to move away from traditional economic thinking as the only way to correct its social and environmental market failures are now endorsing.
Perhaps some of them are the same researchers, countries and organization that in *2012 UNCSD Rio +20 conference The Future We Want were endorsing the SCIENCE BASED decision to go green markets, green economies and green growth to solve the environmental market failure embedded in the pricing mechanism of the traditional market.
THEN THEY apparently forgot that, and they are now PROMOTING THE PROBLEM AS THE SOLUTION, BUT THIS TIME THE PROBLEM IS CIRCULAR, an apparent contradiction.
Keep in mind that the environmental market failure associated to the traditional market that go uncorrected by going circular economy MEANS now that under CIRCULAR ECONOMY THINKING we formally have a life under a PERMANENT MARKET FAILURE that is profitable for those polluting and for those cleaning after them.
Is this science or ideology? Can the root cause of a sustainability problem be made the solution to that problem just by making the problem circular?
And this raises the question: Is the 2023 circular economy push perfect academic tunneling?
I think yes. What do you think?
Any papers on this would be appreciated.
The WCED 1987 documented that business as usual was socially and/or environmentally irresponsible and needed to be made socially and/or environmentally responsible by means beyond traditional economic/development thinking.
The current circular economy thinking appears directed at magically, without addressing the root causes of social and/or environmental problems highlighted by the WCED 1987/Our Common Future, making the irresponsible traditional market thinking responsible just by making it circular.
A linear pollution production problem is solved by a circular pollution production problem apparently, do you see the signs of an academic paradox/contradiction?, which raises the question: Can an irresponsible market/the problem be made responsible/the solution just by making the problem circular?
If you think yes, why? If you think no, why?
I think No!
What are the characteristics of a green university?
I want to analyze the biomedical properties of green synthesized nanoparticles.
Every time i made that, the solution was yellow but last time it became green.
How 555 nm called yellow green sensation be used in protective safety elements to safeguard from enemies?
How is the direction of the electric field related to the green equipotential lines and relation between field lines and direction of electric field?
Two trends dominate the 21C economy: Artificial Intelligence and a switch to the green BioEconomy.
Hello everyone,
I'm currently working with TLC plates stained with NP/PEG and I've noticed distinct yellow and green bands, indicating the presence of flavonoids. I understand that both colors generally signify the presence of flavonoids, but I'm curious if there is any information on whether the green bands might be associated with a specific type of flavonoid, and similarly, if the yellow bands correspond to another type of flavonoid.
Any insights or references on this matter would be greatly appreciated! Thanks in advance.
Specifically, I would like to determine the concentration of Marinobacter hydrocarbonoclasticus. Just like a UV-Vis, I will be using a GL55 photoresistor (as shown in the image attached) to calibrate the concentration of this bacteria by using a laser source. The choice I have available here is either 532nm (green) or 630nm (red) laser. I have found that 532nm is more likely to cause photobleaching to the photoresistor in the long run, since it has a higher photon energy than 630nm, however 532nm can be more sensitive to determine bacteria in a low concentration range. Hence, I would kindly like to seek advice from someone who has experience in developing a system involving LDR coupled with a laser. Thanks in advance!
when we do synthesis of nanoparticle through Green route, we always notice the initial color change by adding Plant Extract and the Salt like Zinc acetate in ZnO NPs etc. and if the color change occur we say our NPs synthesized. so what's the logic behind this, why the color change occurred.
The first round: Where and to whom do we give it and how do we improve green learning skills
Like- I have done various projects during my undergradation, including synthesizing Silver magnetic nanoparticles using potato extract and Gold nanoparticles using neem and tea extract.
For reference to the use of green chemistry in nanotechnology, please find my article below.
Cu-Cr co-substituted M-type barium hexaferrites, synthesized through green methods, demonstrated favorable structural, thermal, magnetic, and dielectric properties. The XRD analysis revealed the formation of M-phase and a secondary BaFe2O4 phase. Raman spectroscopy confirmed successful Cu-Cr ion substitution, maintaining hexagonal morphology. The hard magnetic nature, multi-domain structures, and low-frequency characteristics, especially in the x=0.2 composition, suggest promising applications for lossless low-frequency technologies, opening avenues for practical utilization.
#MaterialScience #Research
For more exploration:
I am researching on ESG principles impact on SME. As for now, most of businesses are shifting towards eco-innovation, green economy and circular economy.
Therefore I am seeking the understanding in this three components which are all complimenting towards ESG practices. Are the three components compliment each other or is one of a subset of on another
I'm conducting a research on corrosion using green inhibitors, and I'm looking at adding electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization (PDP) studies to my work. Having come across so many journals published by Nigerian authors that have done these electrochemical analysis, I was of the thought that it can be achieved locally but my search has proved abortive in search of a reliable research centre where I can do these analysis.
I would sincerely appreciate it if anyone can point me the direction of where I can successfully carry out this analysis.
Thank you all.
What are the most prominent economic effects of green investment?
Can jars contaminated with DNA Green Viewer gel be washed? Is DNA Green Viewer safe to autoclave? Or are these containers considered hazardous waste?
I have synthesis by mixing 10 mM AgNo3 and curry leaf extract
Avoiding the shift from perfect traditional market thinking to perfect green market thinking since 2012 RIO +20 has created a deep green market paradigm shift knowledge gap.
Flipping perfect traditional market thinking to imperfect dwarf green market thinking since 2012 to avoid the shift to perfect green markets has created a deep dwarf green market paradigm flip knowledge gap too.
These knowledge gaps are apparently helping those researchers and institutions implementing development under permanent environmental market failure as well as confusing environmental stakeholders on proper place for action and protest as the responsibility of governments, of businesses and of consumers are changed, and even inversed depending on the market in question.
And this raises the question, green market paradigm shift knowledge gaps and dwarf green market paradigm flip knowledge gaps, are they academic tunneling/willful blindness push helpers?
What do you think?
Generating food for thoughts:
It seems that the capitalist world does not realize yet that green marxism is a bigger threat than red marxism was to capitalism as usual as this time it is coming from within.
And avoiding going green markets since 2012 has played well, and it will continue to increasingly play well for the green marxism claim as pretending to do something when the situation is getting worse may backfire, which raises the question: What comes next after the fall of dwarf green markets, green marxism or green markets?
What do you think?
I am trying to load calcium Green Tm AM inside the neurons. As a first step, I would like to ensure the dye permeates the cell membrane. I am using a 5x and 10x objective to quantify the background fluorescence, then, I inject 200ul of a solution containing the dye and get some pictures post-injection. There is a difference from the background image, but I don’t have a cellular resolution. Would anyone have any suggestions on how I could approach this? I am not sure, for example, whether I can image the dye (it could also be fluo-4, I have a number of those, and I am trying to establish a protocol to check their levels of cell permeability) post PFA fixation using a confocal microscope.
Thank you very much for your time!
How does digitalization helps SME'S to go green ? Do share example related to universities and colleges.
In September this year, I noticed an orange/green coloured scum layer in a slow-running river (see pictures) close to my hometown (Deinze, Belgium) containing green and orange clusters, as can be seen at the close-range picture of the bloom.
Microscopical analysis revealed a micture of Microcystis-colonies (a bloom-forming cyanobacterium, the green part of the scum layer) and an unknown organism of which I have no clue what it could be. I encountered a similar (mixed) bloom about 10 years ago in a recreative lake in Flanders (see report-snippet in attachment).
It might be some micro-algae, or spores/pollen (but in this season?) infected by an aquatic fungus, or both structures (cells and what looks like hyphae) might concern the same organism (a fungus?). I however doubt it concerns a micro-algae.
Since than, I received more records of such scum layers from the nearby river Scheldt, and I personally saw something quite similar in the river Moldau at Prague three weeks ago. I hope someone familiar with this organism can tell me what it is?
Many thanks!
Jeroen
+3
Distinguish between green manuring and crop residue management, and discuss their respective roles in sustainable agriculture practices.
I have synthesized Fe3Se2CO9 by modified Hieber synthesis method...But I am getting a green colour cluster along with the red coloured Fe3Se2CO9 clusters...This is minimizing the yield..How to avoid it??
I am getting only three colors blue, green and red. so sometimes it become difficult to identify. How can we change this default condition?
Is renewable energy effective in promoting growth and how clean energy is used in green economy?
One of the ways for sequestration of CO2 from the atmosphere is, after somehow separating it from the air, to react it with H2 - to get CH4 that can be used as an energy source. The H2 is obtained mainly by water electrolysis, using for that -electricity.
OBTAINING THAT ELECTRICITY - creates, in principle - CO2 that goes to the atmosphere
So the true amount of CO2 sequestration has to take that CO2 creation into the calculation. (Usually, it is said that "green", not from a polluting source will be used, but, since in principle, green H2 could be used directly as an energy source - reducing the amount of energy from polluting sources - it is not really an answer)
So I will be very grateful if you can give that number - How many moles of CO2 are created in a "dirty" electrical plant Coal/gas/ hydrocarbons for producing the electricity amount needed to produce a mol of H2 - say by electrolysis
thanks all for the attention
Yosi scolnik
What is the biggest challenge to sustainability and how it can be resolved and challenges of green strategic approach?
Some colonies on M9 salt glucose agar and Ashby's Sucrose Agar.
Are renewable energy sources clean and green and why is green energy important in sustainable development?
I have been using a PCR master mix green coloured from a Promega ( green taq). But for my recent work in a particular gene that PCR master mix is not working. I borrowed Phusion DNA polymerase ( high fidelity Cat no: F-530XL) from a neighbouring lab for two samples and the amplification did take place. My aim is to amplify the gene promoter region ( PCR product size: 269 bp) and study Single nucleotide polymorphism in the region using RFLP technique. For this purpose I have introduced an restriction cut site ( AluI) near the 3' end of my Reverse primer ( single basepair mismatch) . While checking the details of this phusion DNA polymerase I found it has 3'-5' exonuclease activity and it is known for its accuracy. Hence I am anticipating that there is a chance that the polymerase might cut the error basepair for editing and accuracy maintenance. If this happens then I will have false results for my SNP study. I have never used a high fidelity polymerase before, I used taq polymerase which does not have 3'-5' exonuclease activity. In this regard I am confused which polymerase should I buy. I have seen in thermo catalogue there are normal taq polymerases , dreamtaq as well as many other options but I am unaware about their properties and which one to select. I donot have access to more phusion DNA polymerase or any other taq pol at this moment. This particular gene amplification has been complicated for from the beginning. I have 300+ patient DNA samples to look for SNPs so I am looking for an economic option as well. Kindly guide me what my options are regarding this and which polymerase should I opt for.
After the great development in information technologies, it has become necessary to know the contribution to the development of green products
Explain the relationship between green economy and sustainable development
HK green eyes are most suitable for the same but they're not available anywhere that's why I'm asking the question.
What is the role of the green movement in maintaining the environment and impact of green energy on environment and sustainable development?
Recently, I found there are three equations using in calculating CN(curve number) that area "S= 25400/CN - 254" and "S = 1000/CN - 10" and "S= 2540/CN - 25.4". In fact, many papers used different equations in their research(just more than these three). I guess maye "S= 2540/CN - 25.4" can be misspelling or not. But I'm wondering the difference bettween "S= 25400/CN - 254" and "S = 1000/CN - 10" and whichis the original one.
Why green management is important to sustainability and what is green marketing an emerging approach to sustainable development?
I'm using a phosphate assay kit from a company, which doesn't divulge the specifics of what's in their reagents. To prepare it, we mix a yellow-colored solution with a very small volume of clear solution. I'm trying to understand which solution has the molybdate or the malachite green, or whether they're already mixed together beforehand. Can anyone help me based on this information?
I want to know about the mechanism through which biological nitrogen fixation occurs in photosynthetic green microalgae apart from previously reported bacterial associations. Like in axenic lab conditions ?
What is green economy and how it can bring environmental sustainability and why green energy is important for the sustainability of resources?
What is the green growth strategy in India and how can we achieve green economy?
What are the most common investment objectives, financing what type of green business ventures can be implemented in the formula of green financing offered by banks?
What investment purposes, financing what kind of green economic ventures can be realized in the formula of green financing, which recently in the form of green loans, green leasing, green investment funds, etc. are offered by commercial and investment banks?
Banks, wanting to move with the times, are modifying their offers of financial products and services, including banking, adapting them to changing social trends and customer preferences. Modifications of banking offers mainly concerning the issue of organization, the channel of access to offers are also determined by the technological progress taking place. On the other hand, in terms of changes regarding social trends, changes in customers' worldview, customers' awareness of certain topics considered current and developing, it is the banks that are now recognizing the increasing level of customers' awareness of the climate change taking place, the ongoing process of global warming, the role of humans in these processes, potential threats to the biosphere and people in the future if these processes continue, the need to urgently carry out a green transformation of the economy, to build a green closed loop economy, a sustainable and zero-carbon economy, a sharing economy, the implementation of sustainable development goals. Accordingly, banks, on the one hand, for part of their loan offerings, give names to green loans, which they provide as investment business loans for borrowers planning to implement a green business venture involving, for example, setting up photovoltaic panels on the roof of a house, apartment building, store, business, etc, purchasing an electric or hydrogen-powered car, setting up a household sewage treatment plant, a rainwater catchment system for watering the lawn, building a composter next to the home garden, building a biogas plant producing biogas for energy needs from organic and post-agricultural waste, insulating the facade of a residential building to increase the scale of savings in energy consumption, setting up a system of intelligent blinds to optimize the temperature of the building and increase energy independence, building a system to optimize waste sorting and increase the scale of recycling, building a retention reservoir in an area threatened by long-term drought, carrying out a zoning change investment to reduce the area covered with concrete or asphalt and increase the area of greenery in the city, etc. On the other hand, companies, financial institutions, including commercial banks are modifying their missions and development strategies by adding green business issues, environmental social responsibility, sustainable development goals and fashionable green slogans. Some enterprises, companies and banks also in advertising spots, videos promoting the organization's logo present this logo in the form of an animation that suggests the changes already made in the organization, that the company, enterprise or bank has become green in terms of mission and strategy, that it pursues certain selected or all sustainability goals. But this is not always consistent with the facts. Increasingly, it is greenwashing. The scale of greenwashing is growing rapidly because there is no system for verifying and certifying business entities on the question of whether they are indeed green, whether they are pursuing sustainability goals, at what scale they are doing so in the context of their overall business operations. However, some of the banks that offer, for example, green loans actually finance with their help the green business ventures that the borrowers implement. But, after all, in a significant part of the banks that modify their offerings of banking products in this way, including loans they are still only realizing what they have been doing for many years. The only difference is that previously, before the modification of the bank's offer, the financing of the borrower's project of setting up a photovoltaic panel on the roof of the house or buying an electric car was done by the bank through the granting of an investment loan and not, as now, the granting of a green investment loan. In addition, the difference is also only that green technologies are rapidly developing and becoming cheaper. Until a dozen years ago, the purchase of an electric car or the installation of a photovoltaic panel on the roof of a residential building involved a much higher expense or was practically unaffordable for citizens with an average income level. However, much has been changing in this regard in recent years, and as a result, banks are also modifying their banking product offerings and adding the issues of ecology, green transformation and implementation of sustainable development goals to their missions and development strategies. With increasing competition among banks on the issue of green banking product offerings, it is becoming more and more common for individual banks, as part of advertising campaigns, in advertising spots and promotional videos introducing their green banking products, to also give examples of a specific type of green business venture that can be financed with, for example, a specific type of green loan or green lease. In view of the above, I give one example: One bank in Poland advertises green financing in the form of photovoltaic leasing for companies and SMEs, as well as leasing an electric car. What other similar examples of advertising green banking products and/or financing certain types of green business ventures with them are used by commercial banks?
In view of the above, I address the following question to the esteemed community of scientists and researchers:
What investment objectives, financing of what kind of business ventures can be implemented in the formula of green financing, which recently in the form of green loans, green leasing, green investment funds, etc. are offered by commercial and investment banks?
Most often for what kind of investment purposes, financing what kind of green business ventures can be realized in the formula of green financing offered by banks?
Most often for what kind of green business ventures do commercial banks provide green loans?
Please answer,
I invite everyone to join the discussion,
Thank you very much,
Best wishes,
Dariusz Prokopowicz
Counting on your opinions, on getting to know your personal opinion, on a fair approach to the discussion of scientific issues, I deliberately used the phrase "in your opinion" in the question.
The above text is entirely my own work written by me on the basis of my research.
In writing this text I did not use other sources or automatic text generation systems.
Copyright by Dariusz Prokopowicz
Do you think that for sustainable development, Green Hydrogen can prove to be a game changer?